Gloeobacter violaceus (strain ATCC 29082 / PCC 7421)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Gloeobacteria; Gloeobacterales; Gloeobacteraceae; Gloeobacter; Gloeobacter violaceus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4406 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7NK40|Q7NK40_GLOVI Gll1640 protein OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=gll1640 PE=4 SV=1
MM1 pKa = 7.21EE2 pKa = 5.26EE3 pKa = 5.22KK4 pKa = 10.95YY5 pKa = 10.65FLQNEE10 pKa = 4.41SDD12 pKa = 3.75GSGDD16 pKa = 3.04ATYY19 pKa = 10.46IGTLGEE25 pKa = 4.22EE26 pKa = 4.75GAQVNAFSQNGEE38 pKa = 3.85YY39 pKa = 10.41SYY41 pKa = 11.85GSDD44 pKa = 3.58GNQNVLWDD52 pKa = 3.86QDD54 pKa = 3.07SDD56 pKa = 4.48YY57 pKa = 11.7YY58 pKa = 11.29SVQNLYY64 pKa = 10.82ASEE67 pKa = 4.97DD68 pKa = 3.85GSSLDD73 pKa = 4.04SGTGSTNFGNFEE85 pKa = 4.17NAWISGSASLSDD97 pKa = 3.61NEE99 pKa = 4.66QDD101 pKa = 3.1IDD103 pKa = 4.2TNSDD107 pKa = 3.47VYY109 pKa = 11.51DD110 pKa = 4.65SIAQGVSGLDD120 pKa = 3.33FDD122 pKa = 5.61FDD124 pKa = 4.21SFSDD128 pKa = 3.56ANGTVFATNADD139 pKa = 4.26SPNSNYY145 pKa = 10.04PGADD149 pKa = 2.64TYY151 pKa = 11.89GAMNDD156 pKa = 3.31DD157 pKa = 5.15GYY159 pKa = 11.61RR160 pKa = 11.84LLPGASAVTDD170 pKa = 3.46SAIPQFEE177 pKa = 4.09QAATDD182 pKa = 3.33QDD184 pKa = 4.15LPLAADD190 pKa = 3.62EE191 pKa = 4.79LLVVDD196 pKa = 4.78AQIEE200 pKa = 4.42VEE202 pKa = 4.01NDD204 pKa = 2.32IHH206 pKa = 7.41ALLVSSGEE214 pKa = 3.9QASIAFSLPQGANADD229 pKa = 3.83FMNDD233 pKa = 3.19SSVMAIAKK241 pKa = 9.02WDD243 pKa = 3.76EE244 pKa = 4.29LGGSSNGQTDD254 pKa = 2.87SWNADD259 pKa = 3.74EE260 pKa = 5.47LNVIAGKK267 pKa = 10.49LPAGPNGYY275 pKa = 9.13PDD277 pKa = 5.51DD278 pKa = 3.89KK279 pKa = 10.7TCRR282 pKa = 11.84LLHH285 pKa = 6.12EE286 pKa = 5.41LEE288 pKa = 4.2QKK290 pKa = 11.06YY291 pKa = 10.5PDD293 pKa = 3.63TPQQKK298 pKa = 9.2RR299 pKa = 11.84RR300 pKa = 11.84EE301 pKa = 4.07EE302 pKa = 4.02EE303 pKa = 3.78RR304 pKa = 11.84RR305 pKa = 11.84SMEE308 pKa = 3.88QITYY312 pKa = 9.34PGAGGMLGTIVGSGLTGAAAGSAAGSLGFGIGGLVGGAIGGLAGTIAGEE361 pKa = 4.01QASQDD366 pKa = 3.91KK367 pKa = 10.83VWVEE371 pKa = 3.9DD372 pKa = 3.98AYY374 pKa = 10.21KK375 pKa = 10.35HH376 pKa = 6.48CEE378 pKa = 4.06EE379 pKa = 5.23QGTQAPWTRR388 pKa = 11.84PEE390 pKa = 3.89PPRR393 pKa = 5.45

Molecular weight: 41.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7NKK7|Q7NKK7_GLOVI Gll1470 protein OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=gll1470 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 8.95RR3 pKa = 11.84TLGGTTRR10 pKa = 11.84KK11 pKa = 9.29RR12 pKa = 11.84QKK14 pKa = 9.42TSGFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TASGRR28 pKa = 11.84RR29 pKa = 11.84VLSARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84HH40 pKa = 4.58RR41 pKa = 11.84LAVV44 pKa = 3.46

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4406

0

4406

1377677

28

3277

312.7

34.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.172 ± 0.046

1.024 ± 0.015

4.941 ± 0.029

6.146 ± 0.047

3.734 ± 0.025

8.331 ± 0.04

1.87 ± 0.018

4.433 ± 0.027

2.889 ± 0.033

11.419 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.733 ± 0.015

2.71 ± 0.029

5.654 ± 0.04

4.182 ± 0.028

7.41 ± 0.044

5.485 ± 0.028

5.178 ± 0.028

7.548 ± 0.031

1.503 ± 0.019

2.638 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski