Phormidium tenue NIES-30

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Oscillatoriaceae; Phormidium; Phormidium tenue

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4999 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U7J7B5|A0A1U7J7B5_9CYAN Uncharacterized protein OS=Phormidium tenue NIES-30 OX=549789 GN=NIES30_07370 PE=4 SV=1
MM1 pKa = 8.1DD2 pKa = 5.4DD3 pKa = 3.02WSKK6 pKa = 11.23QLFDD10 pKa = 3.76AFGNAVQDD18 pKa = 3.63FTQQVSEE25 pKa = 4.29DD26 pKa = 3.45AEE28 pKa = 3.9RR29 pKa = 11.84WLDD32 pKa = 3.61NLVEE36 pKa = 4.46QIASASDD43 pKa = 3.48ALVQTTDD50 pKa = 2.43QWAEE54 pKa = 3.89QVQEE58 pKa = 4.81AIDD61 pKa = 3.97PEE63 pKa = 3.99IDD65 pKa = 3.66RR66 pKa = 11.84IAVEE70 pKa = 4.49FNHH73 pKa = 5.62VVEE76 pKa = 4.44PLRR79 pKa = 11.84VTVNAEE85 pKa = 3.04IDD87 pKa = 3.89YY88 pKa = 11.16VADD91 pKa = 3.85EE92 pKa = 4.5LSEE95 pKa = 3.96ILGPLLAGLSGIDD108 pKa = 3.13QWFEE112 pKa = 3.92EE113 pKa = 4.52VSGPFNSTVEE123 pKa = 4.31PLLQNYY129 pKa = 6.98PACVGCRR136 pKa = 11.84NFCGQSYY143 pKa = 10.66GGNTLVCAMHH153 pKa = 7.09PYY155 pKa = 10.13GPEE158 pKa = 4.0EE159 pKa = 4.24EE160 pKa = 4.45RR161 pKa = 11.84QCPDD165 pKa = 3.01WEE167 pKa = 4.85SVWPEE172 pKa = 4.13PKK174 pKa = 10.48DD175 pKa = 3.32QQ176 pKa = 4.08

Molecular weight: 19.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U7J3I8|A0A1U7J3I8_9CYAN Glyceraldehyde-3-phosphate dehydrogenase OS=Phormidium tenue NIES-30 OX=549789 GN=NIES30_15465 PE=3 SV=1
MM1 pKa = 7.99DD2 pKa = 5.63SDD4 pKa = 4.31SPQPRR9 pKa = 11.84FSQEE13 pKa = 2.93IKK15 pKa = 10.91ALLEE19 pKa = 4.62RR20 pKa = 11.84IAEE23 pKa = 4.14QPLTLSHH30 pKa = 6.33VLNEE34 pKa = 3.84TAEE37 pKa = 4.27RR38 pKa = 11.84GFSLVIGLLVLPFLFPMPPGFTTILGSACLLLSLQMAVGRR78 pKa = 11.84RR79 pKa = 11.84SPWLPRR85 pKa = 11.84RR86 pKa = 11.84VARR89 pKa = 11.84FQFPSVLAKK98 pKa = 9.86TVLSNLKK105 pKa = 9.63RR106 pKa = 11.84VTRR109 pKa = 11.84VTEE112 pKa = 3.84RR113 pKa = 11.84FVRR116 pKa = 11.84PRR118 pKa = 11.84LPGLANNPSIWHH130 pKa = 6.36INGLCISWLTLLLMSPIPFTNPIPTVGILIFVVATLEE167 pKa = 4.12ADD169 pKa = 3.5GVMMILGYY177 pKa = 10.41VATGLITATFGLAGYY192 pKa = 8.31LLWRR196 pKa = 11.84SPEE199 pKa = 3.95LLQNWFQRR207 pKa = 3.85

Molecular weight: 23.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4999

0

4999

1597586

23

3743

319.6

35.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.227 ± 0.038

0.922 ± 0.012

5.484 ± 0.028

5.771 ± 0.03

3.578 ± 0.024

7.385 ± 0.038

2.021 ± 0.018

5.212 ± 0.027

3.128 ± 0.027

11.457 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.979 ± 0.018

3.161 ± 0.022

5.448 ± 0.035

5.258 ± 0.036

5.903 ± 0.027

5.913 ± 0.028

5.718 ± 0.025

7.162 ± 0.031

1.526 ± 0.015

2.747 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski