Acholeplasma laidlawii (strain PG-8A)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae; Acholeplasma; Acholeplasma laidlawii

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1380 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A9NHW7|A9NHW7_ACHLI [Ribosomal protein S18]-alanine N-acetyltransferase OS=Acholeplasma laidlawii (strain PG-8A) OX=441768 GN=rimI PE=3 SV=1
MM1 pKa = 7.47KK2 pKa = 9.9KK3 pKa = 9.95ISIIFTLLMTLVLIACTEE21 pKa = 3.85LTAYY25 pKa = 9.91KK26 pKa = 10.09IVFEE30 pKa = 4.59TNGGNDD36 pKa = 2.79IQAIEE41 pKa = 4.21FEE43 pKa = 4.34KK44 pKa = 10.72SYY46 pKa = 11.61ALAEE50 pKa = 4.11IEE52 pKa = 4.27KK53 pKa = 10.31VVPEE57 pKa = 3.91KK58 pKa = 10.72SGYY61 pKa = 7.79TFNSWYY67 pKa = 10.7LEE69 pKa = 3.79SDD71 pKa = 3.44LNEE74 pKa = 4.13GSKK77 pKa = 10.93LSTDD81 pKa = 2.88ITSSVTLYY89 pKa = 11.05AKK91 pKa = 8.33WTITEE96 pKa = 4.08YY97 pKa = 10.75TITLHH102 pKa = 7.04LDD104 pKa = 3.26GGVIHH109 pKa = 7.14PEE111 pKa = 3.86QVSKK115 pKa = 9.14FTILDD120 pKa = 3.73EE121 pKa = 4.66VNLLTPTKK129 pKa = 10.38EE130 pKa = 4.01GFKK133 pKa = 10.73FLGWSISNTEE143 pKa = 3.56ASFVDD148 pKa = 3.72KK149 pKa = 11.52VEE151 pKa = 4.3VGSTEE156 pKa = 3.96HH157 pKa = 6.74KK158 pKa = 10.47SFYY161 pKa = 11.1AKK163 pKa = 9.74WEE165 pKa = 3.91NLGEE169 pKa = 4.19VEE171 pKa = 4.13TFEE174 pKa = 5.96ISFKK178 pKa = 10.6NHH180 pKa = 6.49DD181 pKa = 4.0NAVLQTVEE189 pKa = 4.28VASGVVPAYY198 pKa = 9.42TGQTPTKK205 pKa = 9.49EE206 pKa = 3.75ATLTHH211 pKa = 5.91TFEE214 pKa = 4.04FTGWDD219 pKa = 3.5KK220 pKa = 11.59AVVAATADD228 pKa = 3.29IVYY231 pKa = 9.59VAQFKK236 pKa = 9.46EE237 pKa = 4.37VPITSGTTFNPALLNSIFGLDD258 pKa = 3.55VYY260 pKa = 11.52ALIPEE265 pKa = 4.69IVTSDD270 pKa = 3.75YY271 pKa = 11.51EE272 pKa = 4.73VIDD275 pKa = 3.8NSNDD279 pKa = 3.19LFNDD283 pKa = 3.63VYY285 pKa = 11.08IDD287 pKa = 3.61VFDD290 pKa = 3.72WTEE293 pKa = 4.09SDD295 pKa = 3.94LMAYY299 pKa = 9.56DD300 pKa = 4.38ALLEE304 pKa = 4.14NLLTYY309 pKa = 10.56DD310 pKa = 4.18ALEE313 pKa = 4.2DD314 pKa = 3.94AYY316 pKa = 10.07ILGEE320 pKa = 4.02LFIYY324 pKa = 10.08LYY326 pKa = 10.9ADD328 pKa = 3.84DD329 pKa = 5.66EE330 pKa = 4.56IVPGSIIYY338 pKa = 10.16GIGIYY343 pKa = 9.91QYY345 pKa = 11.3LEE347 pKa = 4.6DD348 pKa = 4.66EE349 pKa = 4.75TPVDD353 pKa = 3.53PVDD356 pKa = 3.58PVGAPFDD363 pKa = 4.0KK364 pKa = 11.19DD365 pKa = 3.39EE366 pKa = 5.08LNGIFGFDD374 pKa = 3.25IYY376 pKa = 11.55ALLPAIISEE385 pKa = 4.46DD386 pKa = 3.58VLITDD391 pKa = 5.33LSDD394 pKa = 2.97EE395 pKa = 4.67TYY397 pKa = 10.39IEE399 pKa = 4.35VYY401 pKa = 10.92VDD403 pKa = 3.17IFTWLDD409 pKa = 3.12ADD411 pKa = 3.85ADD413 pKa = 4.07AYY415 pKa = 10.84DD416 pKa = 4.14ALLSGSLAYY425 pKa = 10.26DD426 pKa = 3.55ATEE429 pKa = 4.08DD430 pKa = 3.6AYY432 pKa = 10.22KK433 pKa = 10.81LGDD436 pKa = 3.41YY437 pKa = 9.67FAYY440 pKa = 9.82IFIDD444 pKa = 3.51EE445 pKa = 4.22EE446 pKa = 4.91TYY448 pKa = 10.63PGLTVFGLAIYY459 pKa = 10.0GDD461 pKa = 3.92KK462 pKa = 11.24AGTTPVDD469 pKa = 3.29PVDD472 pKa = 3.62PVGAPFDD479 pKa = 4.0KK480 pKa = 11.19DD481 pKa = 3.39EE482 pKa = 5.08LNGIFGFDD490 pKa = 3.25IYY492 pKa = 11.51ALLPAIVSEE501 pKa = 4.61DD502 pKa = 3.57VLITDD507 pKa = 5.26LSDD510 pKa = 3.1EE511 pKa = 4.82TYY513 pKa = 10.73VEE515 pKa = 4.46VYY517 pKa = 11.01VDD519 pKa = 3.41IFTWLDD525 pKa = 3.12ADD527 pKa = 3.85ADD529 pKa = 4.07AYY531 pKa = 10.84DD532 pKa = 4.14ALLSGSLAYY541 pKa = 10.26DD542 pKa = 3.55ATEE545 pKa = 4.08DD546 pKa = 3.6AYY548 pKa = 10.22KK549 pKa = 10.81LGDD552 pKa = 3.41YY553 pKa = 9.67FAYY556 pKa = 9.82IFIDD560 pKa = 3.51EE561 pKa = 4.22EE562 pKa = 4.91TYY564 pKa = 10.63PGLTVFGLAIYY575 pKa = 10.0GDD577 pKa = 3.92KK578 pKa = 11.24AGTTPVDD585 pKa = 3.45PVEE588 pKa = 4.22PEE590 pKa = 3.18IGEE593 pKa = 4.27YY594 pKa = 11.06YY595 pKa = 10.59SFNVQDD601 pKa = 3.68TTSTLDD607 pKa = 3.05GSYY610 pKa = 10.73RR611 pKa = 11.84NNIDD615 pKa = 3.1VTLNFANNTNKK626 pKa = 10.01VIVKK630 pKa = 9.87ASHH633 pKa = 6.64IANITQTAPGGLSLGKK649 pKa = 10.0IFAANVSGNANPTVYY664 pKa = 10.91LEE666 pKa = 4.09IDD668 pKa = 3.2ALGNLIDD675 pKa = 3.84TMSFEE680 pKa = 4.19IQGRR684 pKa = 11.84TGFSPNLAGAKK695 pKa = 9.8LQVFNNGVWTDD706 pKa = 3.41LAGGNFYY713 pKa = 11.08SQIASSKK720 pKa = 8.41TLITISGINASKK732 pKa = 10.35FRR734 pKa = 11.84LLFQGTGATSNGGQFMIFNVNLLTGNAPAPVYY766 pKa = 9.29EE767 pKa = 4.3LWSDD771 pKa = 3.69VVTDD775 pKa = 4.94LEE777 pKa = 5.02AKK779 pKa = 10.42FDD781 pKa = 4.18DD782 pKa = 4.96LDD784 pKa = 4.3FNTYY788 pKa = 9.55MPDD791 pKa = 3.81FADD794 pKa = 3.65LTNLKK799 pKa = 7.99VTKK802 pKa = 10.68VSDD805 pKa = 3.22KK806 pKa = 10.31SFKK809 pKa = 10.77VVGSTTLDD817 pKa = 3.3VNTLYY822 pKa = 10.58TSYY825 pKa = 11.33INLILNKK832 pKa = 10.1SFEE835 pKa = 4.55KK836 pKa = 10.89NDD838 pKa = 3.65DD839 pKa = 3.51LSLVRR844 pKa = 11.84GHH846 pKa = 6.97DD847 pKa = 3.47VYY849 pKa = 11.73VYY851 pKa = 10.56VVNDD855 pKa = 3.73DD856 pKa = 3.84LAYY859 pKa = 10.74AMYY862 pKa = 9.92IIKK865 pKa = 8.86GTEE868 pKa = 3.74SLEE871 pKa = 3.99VYY873 pKa = 9.91IYY875 pKa = 10.83QFDD878 pKa = 3.7AVMDD882 pKa = 4.24DD883 pKa = 3.95VVLEE887 pKa = 4.28TLSKK891 pKa = 9.59RR892 pKa = 11.84QSINEE897 pKa = 4.0YY898 pKa = 9.69EE899 pKa = 4.21VSQFGMSGLPSTGTYY914 pKa = 10.42DD915 pKa = 3.51VLVVPVEE922 pKa = 4.0IQNVPFEE929 pKa = 4.43ASYY932 pKa = 8.51KK933 pKa = 9.13TKK935 pKa = 10.5LDD937 pKa = 3.28KK938 pKa = 11.34VFNGTSLDD946 pKa = 3.88TGWEE950 pKa = 4.49SVSSYY955 pKa = 10.64YY956 pKa = 10.64YY957 pKa = 10.36KK958 pKa = 10.85SSFGLLDD965 pKa = 4.27LNFDD969 pKa = 3.84ILDD972 pKa = 3.48KK973 pKa = 11.13HH974 pKa = 5.19VTSNVKK980 pKa = 10.27AFYY983 pKa = 9.81EE984 pKa = 4.6GKK986 pKa = 10.43GQDD989 pKa = 3.3GDD991 pKa = 3.54QYY993 pKa = 11.78AILDD997 pKa = 4.16ALTALDD1003 pKa = 3.71STIDD1007 pKa = 3.62FSKK1010 pKa = 11.1YY1011 pKa = 10.19DD1012 pKa = 3.71SNNDD1016 pKa = 3.22GVIDD1020 pKa = 4.37SIIFIYY1026 pKa = 9.26STDD1029 pKa = 3.24YY1030 pKa = 11.43NYY1032 pKa = 11.25DD1033 pKa = 3.36VDD1035 pKa = 4.85PWWAWVYY1042 pKa = 10.77VSNPDD1047 pKa = 3.0IVGSVSEE1054 pKa = 4.31LDD1056 pKa = 3.53GKK1058 pKa = 10.42NFEE1061 pKa = 4.51YY1062 pKa = 10.9YY1063 pKa = 10.13FWASYY1068 pKa = 11.68DD1069 pKa = 3.6FMNDD1073 pKa = 3.28ALPGNSDD1080 pKa = 4.96LILNSEE1086 pKa = 4.5TYY1088 pKa = 9.82IHH1090 pKa = 6.7EE1091 pKa = 5.01LGHH1094 pKa = 7.61LMGIVDD1100 pKa = 4.49FYY1102 pKa = 10.84PYY1104 pKa = 10.45EE1105 pKa = 4.38GNNQYY1110 pKa = 11.24GPMGGFDD1117 pKa = 3.37MMDD1120 pKa = 3.84YY1121 pKa = 10.91NAGDD1125 pKa = 3.51HH1126 pKa = 6.8GPFNKK1131 pKa = 10.17LVFGWLQPLVAQKK1144 pKa = 9.29GTYY1147 pKa = 9.1QVTLDD1152 pKa = 4.19SYY1154 pKa = 11.31STDD1157 pKa = 3.3TDD1159 pKa = 3.84GLNSTLLIPFNSSDD1173 pKa = 4.2LNDD1176 pKa = 3.32GNAFDD1181 pKa = 4.98EE1182 pKa = 4.47YY1183 pKa = 10.66LLVMFYY1189 pKa = 10.35TPNGLYY1195 pKa = 9.93SAHH1198 pKa = 6.86SGLEE1202 pKa = 4.11YY1203 pKa = 10.37IPSNAGIVVYY1213 pKa = 10.52HH1214 pKa = 6.56IDD1216 pKa = 4.49ARR1218 pKa = 11.84LTSNPVFWGEE1228 pKa = 3.67YY1229 pKa = 8.97FRR1231 pKa = 11.84NNNEE1235 pKa = 4.02GASSFINQILEE1246 pKa = 3.86ADD1248 pKa = 4.17KK1249 pKa = 11.52NNSIPGNGSIKK1260 pKa = 10.56QSDD1263 pKa = 4.4LLTSGTLNLNTYY1275 pKa = 8.9SWNQGGKK1282 pKa = 9.45INVSLTVASLFNNSSEE1298 pKa = 4.1NVTLTVSVAA1307 pKa = 3.15

Molecular weight: 144.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A9NE66|A9NE66_ACHLI IS-10 transposase OS=Acholeplasma laidlawii (strain PG-8A) OX=441768 GN=ACL_0003 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.95QPSKK9 pKa = 9.3IKK11 pKa = 9.77HH12 pKa = 4.29QRR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.25GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 8.93VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.24GRR39 pKa = 11.84QSLTVV44 pKa = 3.17

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1380

0

1380

451261

25

5552

327.0

37.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.75 ± 0.063

0.351 ± 0.012

6.011 ± 0.046

6.355 ± 0.05

4.843 ± 0.049

6.006 ± 0.079

2.089 ± 0.036

8.963 ± 0.064

7.82 ± 0.089

10.006 ± 0.089

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.55 ± 0.031

5.534 ± 0.071

3.045 ± 0.032

3.106 ± 0.037

3.336 ± 0.048

6.139 ± 0.044

5.922 ± 0.075

6.846 ± 0.055

0.737 ± 0.023

4.591 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski