Bombilactobacillus mellifer

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Bombilactobacillus

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1592 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F4LV45|A0A0F4LV45_9LACO Teichoic acid D-alanyltransferase OS=Bombilactobacillus mellifer OX=1218492 GN=JG30_04260 PE=3 SV=1
MM1 pKa = 6.88TQQSEE6 pKa = 4.57NNVDD10 pKa = 3.52QEE12 pKa = 4.74IILSDD17 pKa = 3.8EE18 pKa = 4.07QGHH21 pKa = 4.21QARR24 pKa = 11.84YY25 pKa = 9.76KK26 pKa = 10.4ILFTFDD32 pKa = 3.16SEE34 pKa = 5.59DD35 pKa = 3.42YY36 pKa = 10.25GKK38 pKa = 10.85SYY40 pKa = 11.07VLLYY44 pKa = 10.59LASQEE49 pKa = 3.9NAAEE53 pKa = 4.11IEE55 pKa = 3.98IQAFSFQPDD64 pKa = 3.18ANGDD68 pKa = 3.59VTAGDD73 pKa = 4.48LEE75 pKa = 4.92PIEE78 pKa = 6.14DD79 pKa = 4.09DD80 pKa = 5.29DD81 pKa = 3.79EE82 pKa = 4.23WAMVQEE88 pKa = 4.19VLNTFSAEE96 pKa = 3.98EE97 pKa = 3.87

Molecular weight: 11.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F4LPL0|A0A0F4LPL0_9LACO Pyruvate oxidase OS=Bombilactobacillus mellifer OX=1218492 GN=JG30_14570 PE=3 SV=1
MM1 pKa = 6.27QQPRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84NVYY11 pKa = 9.51PIKK14 pKa = 10.68LNNSDD19 pKa = 4.09ASAEE23 pKa = 4.2NKK25 pKa = 9.37FKK27 pKa = 10.96RR28 pKa = 11.84RR29 pKa = 11.84IIQVHH34 pKa = 5.21RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84ITLLLAVGLVILVALGSRR55 pKa = 11.84VVQAQQEE62 pKa = 4.36RR63 pKa = 11.84EE64 pKa = 3.89QTRR67 pKa = 11.84VEE69 pKa = 4.34FNQHH73 pKa = 4.82QKK75 pKa = 10.77RR76 pKa = 11.84LQQTKK81 pKa = 9.37QKK83 pKa = 10.1QANLQTEE90 pKa = 4.61VRR92 pKa = 11.84QLHH95 pKa = 5.3NPEE98 pKa = 4.22YY99 pKa = 10.45LDD101 pKa = 3.2NLIRR105 pKa = 11.84YY106 pKa = 7.39RR107 pKa = 11.84FNYY110 pKa = 10.4SKK112 pKa = 11.08DD113 pKa = 3.19NEE115 pKa = 4.44IIYY118 pKa = 9.2NIPNEE123 pKa = 4.1ANQNLNFF130 pKa = 4.17

Molecular weight: 15.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1592

0

1592

514180

38

1774

323.0

36.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.259 ± 0.079

0.654 ± 0.017

5.084 ± 0.057

4.425 ± 0.058

4.092 ± 0.043

6.106 ± 0.059

2.425 ± 0.026

7.19 ± 0.059

5.616 ± 0.065

10.496 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.351 ± 0.027

5.049 ± 0.065

3.981 ± 0.033

7.105 ± 0.091

3.82 ± 0.042

5.712 ± 0.049

6.282 ± 0.056

6.749 ± 0.039

1.066 ± 0.023

3.537 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski