Coniophora puteana (strain RWD-64-598) (Brown rot fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Boletales; Coniophorineae; Coniophoraceae; Coniophora; Coniophora puteana

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13735 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5M3MY85|A0A5M3MY85_CONPW Uncharacterized protein OS=Coniophora puteana (strain RWD-64-598) OX=741705 GN=CONPUDRAFT_150798 PE=4 SV=1
MM1 pKa = 8.02RR2 pKa = 11.84FTLATVIAVLPFLVDD17 pKa = 3.94AAPAPAARR25 pKa = 11.84APSNKK30 pKa = 9.19IAIQKK35 pKa = 10.26RR36 pKa = 11.84STLLNVDD43 pKa = 3.25GTVNLTALNRR53 pKa = 11.84HH54 pKa = 4.98TQSIKK59 pKa = 10.61AKK61 pKa = 8.43YY62 pKa = 9.14EE63 pKa = 3.96RR64 pKa = 11.84GFTNYY69 pKa = 10.15AKK71 pKa = 9.46NTGKK75 pKa = 9.58VHH77 pKa = 7.15SLAKK81 pKa = 10.36VNAGKK86 pKa = 10.29RR87 pKa = 11.84DD88 pKa = 3.51TGTVDD93 pKa = 3.27LTDD96 pKa = 4.61DD97 pKa = 5.1SSEE100 pKa = 3.67LWYY103 pKa = 10.88GKK105 pKa = 10.2ISVGTPAVEE114 pKa = 3.97YY115 pKa = 10.02TVDD118 pKa = 4.17FDD120 pKa = 4.24TGSSDD125 pKa = 4.51LFLPGPDD132 pKa = 4.81CDD134 pKa = 4.39DD135 pKa = 3.65TCSGHH140 pKa = 5.81TVYY143 pKa = 10.83DD144 pKa = 3.89PSKK147 pKa = 10.95SSDD150 pKa = 3.58SQDD153 pKa = 3.03VGQTFQLQYY162 pKa = 11.21GDD164 pKa = 3.63GSTVSGEE171 pKa = 3.82QWTDD175 pKa = 2.95TVTLAGLTATEE186 pKa = 4.13QTLGAAQQYY195 pKa = 7.87STGFEE200 pKa = 4.27SAQFPADD207 pKa = 3.13GLMGMAYY214 pKa = 9.41PAISSYY220 pKa = 10.72GASPVFNTLVSQGQTDD236 pKa = 3.24AGVFGFKK243 pKa = 9.26LTSSGAEE250 pKa = 3.83LTIGGVDD257 pKa = 3.47QSAVSGDD264 pKa = 3.46FTYY267 pKa = 11.07APVTQQGYY275 pKa = 5.89WQITADD281 pKa = 3.95GVSSGGQQGVGQFDD295 pKa = 5.29AIADD299 pKa = 3.8TGTTLIVGIPDD310 pKa = 3.69DD311 pKa = 3.57VASFYY316 pKa = 11.02EE317 pKa = 4.6AIGGQDD323 pKa = 3.14ASSTLGEE330 pKa = 4.27GYY332 pKa = 8.52YY333 pKa = 9.38TVPCDD338 pKa = 4.21SIPDD342 pKa = 3.55VTVTIGGQDD351 pKa = 3.62FPVSADD357 pKa = 3.48TFNLGAASQGSSDD370 pKa = 3.92CVGGIVGQDD379 pKa = 3.2LGQGFWILGDD389 pKa = 3.51VFLSNTYY396 pKa = 8.57TAFDD400 pKa = 3.84FDD402 pKa = 4.24NNQVGFAPLAA412 pKa = 4.1

Molecular weight: 42.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5M3MII5|A0A5M3MII5_CONPW Uncharacterized protein OS=Coniophora puteana (strain RWD-64-598) OX=741705 GN=CONPUDRAFT_166778 PE=4 SV=1
MM1 pKa = 7.67IFNKK5 pKa = 10.43LSMLAVAAVMTAAVAAPVPEE25 pKa = 4.54PVTVRR30 pKa = 11.84APVTVRR36 pKa = 11.84SPVTVRR42 pKa = 11.84SPVTVRR48 pKa = 11.84APVTVRR54 pKa = 11.84SPVTVRR60 pKa = 11.84SPVTVRR66 pKa = 11.84SPVTVRR72 pKa = 11.84SPVTVRR78 pKa = 11.84SPVTVRR84 pKa = 11.84SPVTVRR90 pKa = 11.84SPVTVRR96 pKa = 11.84RR97 pKa = 11.84RR98 pKa = 11.84APVTVRR104 pKa = 11.84SPVTVRR110 pKa = 11.84SPVTVRR116 pKa = 11.84RR117 pKa = 11.84RR118 pKa = 11.84APVTVRR124 pKa = 11.84SPVTVRR130 pKa = 11.84SPVTVRR136 pKa = 11.84RR137 pKa = 11.84RR138 pKa = 11.84APVTVRR144 pKa = 11.84SPVTVRR150 pKa = 11.84SPVTVRR156 pKa = 11.84RR157 pKa = 11.84RR158 pKa = 11.84APVTVRR164 pKa = 11.84SPVTVRR170 pKa = 11.84SPVTVRR176 pKa = 11.84SPVTVRR182 pKa = 11.84RR183 pKa = 11.84RR184 pKa = 11.84APVTVRR190 pKa = 11.84SPVTVRR196 pKa = 11.84APVTVRR202 pKa = 11.84SPVTVRR208 pKa = 11.84SPVTVRR214 pKa = 11.84SPVTVRR220 pKa = 11.84RR221 pKa = 11.84RR222 pKa = 11.84SPVTVRR228 pKa = 11.84SPVTVRR234 pKa = 11.84SPVTVRR240 pKa = 11.84RR241 pKa = 11.84RR242 pKa = 11.84APVTVRR248 pKa = 11.84SPVTVRR254 pKa = 11.84SPVTIRR260 pKa = 11.84RR261 pKa = 11.84RR262 pKa = 11.84APVTVRR268 pKa = 11.84SPVTVRR274 pKa = 11.84RR275 pKa = 11.84RR276 pKa = 11.84SPVTVRR282 pKa = 11.84SPVTVRR288 pKa = 11.84SPVTVRR294 pKa = 11.84SPVTVRR300 pKa = 11.84SPVTVRR306 pKa = 11.84SPVTVRR312 pKa = 11.84SPVTVRR318 pKa = 11.84SPVTVRR324 pKa = 11.84SPVTVRR330 pKa = 11.84APVTVRR336 pKa = 11.84AVEE339 pKa = 4.08LDD341 pKa = 3.25

Molecular weight: 36.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13735

0

13735

6006140

49

5304

437.3

48.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.064 ± 0.021

1.308 ± 0.009

5.703 ± 0.016

5.803 ± 0.021

3.646 ± 0.013

6.811 ± 0.023

2.604 ± 0.011

4.616 ± 0.018

4.194 ± 0.018

9.13 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.132 ± 0.008

3.346 ± 0.011

6.357 ± 0.025

3.692 ± 0.015

6.283 ± 0.021

8.667 ± 0.026

5.926 ± 0.013

6.506 ± 0.015

1.509 ± 0.008

2.705 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski