Megavirus chiliensis

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Imitervirales; Mimiviridae; Mimivirus; unclassified Mimivirus

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1120 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G5CRN5|G5CRN5_9VIRU Putative BTB/POZ domain-containing protein OS=Megavirus chiliensis OX=1094892 GN=mg209 PE=3 SV=1
MM1 pKa = 7.76SYY3 pKa = 11.35NDD5 pKa = 4.28FMVSDD10 pKa = 4.37YY11 pKa = 11.62EE12 pKa = 4.52SINYY16 pKa = 9.36NMYY19 pKa = 10.73NITQIDD25 pKa = 3.78MDD27 pKa = 3.72IQNDD31 pKa = 3.52YY32 pKa = 11.37SEE34 pKa = 4.74NFSIYY39 pKa = 9.85IDD41 pKa = 3.45NKK43 pKa = 10.34PIIEE47 pKa = 4.33SPSMGDD53 pKa = 3.3KK54 pKa = 10.67SLPTINFDD62 pKa = 5.22AILDD66 pKa = 3.8RR67 pKa = 11.84TKK69 pKa = 11.02SVFGIMFNPKK79 pKa = 9.75YY80 pKa = 10.7FMPNCRR86 pKa = 11.84EE87 pKa = 3.9LDD89 pKa = 3.51LMDD92 pKa = 5.78RR93 pKa = 11.84EE94 pKa = 4.21IEE96 pKa = 4.34VYY98 pKa = 11.4NMVV101 pKa = 3.45

Molecular weight: 11.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G5CQU8|G5CQU8_9VIRU Uncharacterized protein mg71 OS=Megavirus chiliensis OX=1094892 GN=mg71 PE=3 SV=1
MM1 pKa = 7.47KK2 pKa = 10.28ACNTEE7 pKa = 3.86VGDD10 pKa = 3.95RR11 pKa = 11.84WFEE14 pKa = 3.74RR15 pKa = 11.84CIAYY19 pKa = 9.75HH20 pKa = 7.02RR21 pKa = 11.84FLGWHH26 pKa = 5.77SGSRR30 pKa = 11.84TRR32 pKa = 11.84PFHH35 pKa = 6.6RR36 pKa = 11.84RR37 pKa = 11.84HH38 pKa = 6.09SPWYY42 pKa = 7.2WQFRR46 pKa = 11.84KK47 pKa = 9.93CFRR50 pKa = 11.84LL51 pKa = 3.48

Molecular weight: 6.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1120

0

1120

377837

28

2907

337.4

39.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.043 ± 0.06

1.967 ± 0.051

6.668 ± 0.072

5.089 ± 0.068

4.462 ± 0.05

4.547 ± 0.182

2.218 ± 0.039

11.026 ± 0.096

8.905 ± 0.111

7.984 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.224 ± 0.037

10.024 ± 0.112

3.103 ± 0.06

3.151 ± 0.043

2.902 ± 0.052

6.735 ± 0.071

5.022 ± 0.058

4.278 ± 0.052

0.836 ± 0.028

5.799 ± 0.069
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski