Muribaculaceae bacterium Isolate-102 (HZI)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Muribaculaceae; unclassified Muribaculaceae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2650 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N2N226|A0A3N2N226_9BACT Esterase OS=Muribaculaceae bacterium Isolate-102 (HZI) OX=2486470 GN=EEL48_03405 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.39LHH4 pKa = 6.5IVLFSLLVVTLAGCTAATTTEE25 pKa = 3.93NLAGARR31 pKa = 11.84LLIEE35 pKa = 4.65NNDD38 pKa = 3.5FASAQSMCDD47 pKa = 3.2EE48 pKa = 4.01VRR50 pKa = 11.84NAVEE54 pKa = 4.75SDD56 pKa = 3.53DD57 pKa = 4.48SDD59 pKa = 4.77VEE61 pKa = 4.76VEE63 pKa = 4.68DD64 pKa = 3.84LCKK67 pKa = 10.44LAILYY72 pKa = 7.56MQLADD77 pKa = 3.84HH78 pKa = 7.02TDD80 pKa = 3.13RR81 pKa = 11.84DD82 pKa = 4.42DD83 pKa = 3.67NVEE86 pKa = 4.15YY87 pKa = 10.79ARR89 pKa = 11.84QAYY92 pKa = 7.14MQSLEE97 pKa = 4.2CDD99 pKa = 3.05SAGAMDD105 pKa = 4.38YY106 pKa = 10.89YY107 pKa = 11.37SRR109 pKa = 11.84LGVDD113 pKa = 3.52EE114 pKa = 4.16MAQVVLLSSIVRR126 pKa = 11.84STTAVPEE133 pKa = 3.87IDD135 pKa = 3.84EE136 pKa = 4.48QGDD139 pKa = 3.66TLLIVPDD146 pKa = 4.82DD147 pKa = 4.53DD148 pKa = 5.98SYY150 pKa = 11.44MIPP153 pKa = 3.76

Molecular weight: 16.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N2MZL8|A0A3N2MZL8_9BACT RNA polymerase sigma factor OS=Muribaculaceae bacterium Isolate-102 (HZI) OX=2486470 GN=EEL48_00670 PE=3 SV=1
MM1 pKa = 7.54KK2 pKa = 10.27PNITSRR8 pKa = 11.84DD9 pKa = 3.03KK10 pKa = 11.55DD11 pKa = 4.08FIEE14 pKa = 4.79ACQSVLSHH22 pKa = 6.63CSCDD26 pKa = 3.26SRR28 pKa = 11.84PCMRR32 pKa = 11.84AIVASAITSPAPSYY46 pKa = 10.68YY47 pKa = 10.4VSPDD51 pKa = 3.08YY52 pKa = 11.16AFRR55 pKa = 11.84IMSSYY60 pKa = 10.62LNHH63 pKa = 6.72GKK65 pKa = 10.19LPSRR69 pKa = 11.84RR70 pKa = 11.84LARR73 pKa = 11.84EE74 pKa = 3.36MWLEE78 pKa = 3.82IASKK82 pKa = 7.63VTRR85 pKa = 11.84LRR87 pKa = 11.84RR88 pKa = 11.84EE89 pKa = 4.08NPSMPLIKK97 pKa = 10.39AVTEE101 pKa = 4.22VTANEE106 pKa = 3.76HH107 pKa = 6.13ASRR110 pKa = 11.84FFISDD115 pKa = 3.92GYY117 pKa = 11.28ALRR120 pKa = 11.84LLQRR124 pKa = 11.84RR125 pKa = 11.84NRR127 pKa = 11.84SSRR130 pKa = 11.84LALKK134 pKa = 10.42PNLLSWHH141 pKa = 7.22DD142 pKa = 3.43GGNPFRR148 pKa = 11.84RR149 pKa = 11.84SPRR152 pKa = 11.84HH153 pKa = 5.1IRR155 pKa = 11.84HH156 pKa = 5.66TRR158 pKa = 11.84QSDD161 pKa = 2.95

Molecular weight: 18.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2650

0

2650

924913

29

2449

349.0

39.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.802 ± 0.051

1.201 ± 0.018

6.258 ± 0.038

6.001 ± 0.044

4.135 ± 0.031

6.852 ± 0.041

1.925 ± 0.02

7.072 ± 0.046

5.647 ± 0.046

8.765 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.926 ± 0.023

5.013 ± 0.036

4.015 ± 0.025

2.865 ± 0.025

5.339 ± 0.037

6.499 ± 0.038

5.526 ± 0.032

6.737 ± 0.042

1.261 ± 0.021

4.163 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski