Butyricimonas sp. An62

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Odoribacteraceae; Butyricimonas; unclassified Butyricimonas

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3969 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y3VXT8|A0A1Y3VXT8_9BACT Iron-sulfur cluster carrier protein OS=Butyricimonas sp. An62 OX=1965649 GN=B5G13_04845 PE=3 SV=1
MM1 pKa = 8.45DD2 pKa = 3.59YY3 pKa = 10.54RR4 pKa = 11.84FEE6 pKa = 4.82ISLPTSSDD14 pKa = 3.44FRR16 pKa = 11.84CEE18 pKa = 3.46IAVGGEE24 pKa = 3.6QTFQQFHH31 pKa = 6.75DD32 pKa = 4.95KK33 pKa = 10.47IVEE36 pKa = 4.11TLNYY40 pKa = 9.94DD41 pKa = 3.85SSQMASFFTLDD52 pKa = 2.92RR53 pKa = 11.84MGNRR57 pKa = 11.84VKK59 pKa = 10.61EE60 pKa = 3.97IALMDD65 pKa = 3.65MTTEE69 pKa = 4.12DD70 pKa = 4.4EE71 pKa = 4.37EE72 pKa = 5.17NEE74 pKa = 4.29TLVMDD79 pKa = 3.67NTKK82 pKa = 10.06ISDD85 pKa = 3.9IIKK88 pKa = 9.7PGCLEE93 pKa = 3.98LEE95 pKa = 4.09YY96 pKa = 11.11VYY98 pKa = 11.3DD99 pKa = 4.39FFSNKK104 pKa = 8.23YY105 pKa = 10.05FKK107 pKa = 10.93VEE109 pKa = 3.76YY110 pKa = 9.6AGEE113 pKa = 4.2YY114 pKa = 9.98IRR116 pKa = 11.84DD117 pKa = 3.81SSDD120 pKa = 3.11VMPVCVACEE129 pKa = 3.74GDD131 pKa = 3.29IPEE134 pKa = 4.16QFSYY138 pKa = 11.53EE139 pKa = 4.19DD140 pKa = 4.77GNADD144 pKa = 3.46WEE146 pKa = 4.26FDD148 pKa = 3.66KK149 pKa = 11.45PEE151 pKa = 3.82EE152 pKa = 4.31EE153 pKa = 4.68YY154 pKa = 11.07EE155 pKa = 4.07GDD157 pKa = 3.62YY158 pKa = 11.25DD159 pKa = 4.77DD160 pKa = 6.37SFMEE164 pKa = 4.14EE165 pKa = 4.7FGNGDD170 pKa = 3.44YY171 pKa = 11.02DD172 pKa = 5.29DD173 pKa = 5.48EE174 pKa = 5.07DD175 pKa = 4.23RR176 pKa = 11.84QDD178 pKa = 3.42RR179 pKa = 11.84GGDD182 pKa = 3.29DD183 pKa = 3.1EE184 pKa = 4.93YY185 pKa = 11.8YY186 pKa = 10.17RR187 pKa = 11.84DD188 pKa = 3.72EE189 pKa = 5.17RR190 pKa = 11.84YY191 pKa = 10.14EE192 pKa = 4.08SLDD195 pKa = 3.84DD196 pKa = 4.59YY197 pKa = 11.4IDD199 pKa = 3.58RR200 pKa = 11.84LL201 pKa = 3.76

Molecular weight: 23.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y3W2E2|A0A1Y3W2E2_9BACT Uncharacterized protein OS=Butyricimonas sp. An62 OX=1965649 GN=B5G13_11035 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.11RR3 pKa = 11.84GKK5 pKa = 8.89YY6 pKa = 8.31TILVFAITISILVFLLLNLLLGSVSIPFRR35 pKa = 11.84SVWNILTGGEE45 pKa = 4.2GDD47 pKa = 3.46PVAWQNIILKK57 pKa = 10.26SRR59 pKa = 11.84LPQALTALVAGAGLSISGLQMQTIFRR85 pKa = 11.84NPLAGPSVLGISSGASLGVAFVVLLSGQLGGVALSRR121 pKa = 11.84LGFIGEE127 pKa = 4.27VALSIAAIVGALSVMGLIVLASRR150 pKa = 11.84KK151 pKa = 8.98VAGSVTLLIIGVMIGYY167 pKa = 9.12IANAVIGVLKK177 pKa = 10.21FFSVEE182 pKa = 3.81EE183 pKa = 4.45DD184 pKa = 2.66IKK186 pKa = 11.4AYY188 pKa = 9.99VIWGLGSFARR198 pKa = 11.84VSGNQMMLFVTIMAVIIPLSFLLIKK223 pKa = 10.02PLNLLMLGDD232 pKa = 4.56GYY234 pKa = 11.62ARR236 pKa = 11.84NLGLNINRR244 pKa = 11.84ARR246 pKa = 11.84VWVITCAGVLTAIVTAYY263 pKa = 9.65CGPIIFLGLAVPHH276 pKa = 6.85LCRR279 pKa = 11.84AIFQTSDD286 pKa = 2.79HH287 pKa = 6.46RR288 pKa = 11.84VLMPASMLVGASLALLCNLIARR310 pKa = 11.84LPGFEE315 pKa = 4.07GALPVNSVTALVGAPVVASVLFRR338 pKa = 11.84KK339 pKa = 10.1RR340 pKa = 11.84KK341 pKa = 9.65RR342 pKa = 11.84RR343 pKa = 11.84DD344 pKa = 3.17AVEE347 pKa = 3.86FF348 pKa = 4.01

Molecular weight: 36.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3969

0

3969

1544925

26

1907

389.2

44.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.322 ± 0.034

1.103 ± 0.014

5.58 ± 0.024

6.987 ± 0.035

4.75 ± 0.024

6.72 ± 0.028

1.613 ± 0.015

6.839 ± 0.037

6.767 ± 0.029

9.301 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.77 ± 0.014

5.169 ± 0.031

3.488 ± 0.02

3.315 ± 0.018

4.788 ± 0.029

6.132 ± 0.032

5.474 ± 0.025

7.041 ± 0.031

1.213 ± 0.012

4.626 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski