Mycobacterium phage Manad

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; Pegunavirus; Mycobacterium virus Manad

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059VKM9|A0A059VKM9_9CAUD Uncharacterized protein OS=Mycobacterium phage Manad OX=1486403 GN=68 PE=4 SV=1
MM1 pKa = 6.88FTDD4 pKa = 3.48PRR6 pKa = 11.84IRR8 pKa = 11.84SAFLAGVAAGAALGVTVGAALVLTALPDD36 pKa = 3.92DD37 pKa = 4.34VSVPRR42 pKa = 11.84PGLSVTEE49 pKa = 4.3TVATKK54 pKa = 10.27QFAMWPADD62 pKa = 3.56PAPTPEE68 pKa = 5.55DD69 pKa = 4.42DD70 pKa = 3.69PAFDD74 pKa = 4.19CRR76 pKa = 11.84YY77 pKa = 9.45HH78 pKa = 6.82GNHH81 pKa = 5.92ICGPLNVDD89 pKa = 4.11GYY91 pKa = 11.9APGLYY96 pKa = 9.09IDD98 pKa = 4.99GVLVDD103 pKa = 4.79PWPVDD108 pKa = 3.43PASTPTIEE116 pKa = 4.27VPP118 pKa = 3.66

Molecular weight: 12.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059VKF6|A0A059VKF6_9CAUD Uncharacterized protein OS=Mycobacterium phage Manad OX=1486403 GN=99 PE=4 SV=1
MM1 pKa = 6.61GTKK4 pKa = 10.17AVVQSRR10 pKa = 11.84VINVNQTITSPTGVAKK26 pKa = 10.53ASKK29 pKa = 10.34ARR31 pKa = 11.84EE32 pKa = 3.92TLATARR38 pKa = 11.84EE39 pKa = 3.82ILYY42 pKa = 10.75ASGNMRR48 pKa = 11.84AADD51 pKa = 4.88AITPALHH58 pKa = 7.4DD59 pKa = 4.2LTRR62 pKa = 11.84QIADD66 pKa = 3.5YY67 pKa = 9.98YY68 pKa = 10.42RR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84AEE73 pKa = 3.78ADD75 pKa = 2.69KK76 pKa = 11.0RR77 pKa = 11.84AAQRR81 pKa = 3.36

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

101

0

101

21687

36

1992

214.7

23.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.141 ± 0.348

1.061 ± 0.149

6.465 ± 0.272

5.957 ± 0.366

2.365 ± 0.111

9.632 ± 0.673

2.024 ± 0.203

4.602 ± 0.185

2.965 ± 0.171

7.954 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.089 ± 0.099

2.9 ± 0.242

6.114 ± 0.242

3.066 ± 0.135

6.741 ± 0.376

4.929 ± 0.219

7.078 ± 0.179

7.594 ± 0.204

1.817 ± 0.161

2.504 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski