Streptomyces sp. NTH33

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7049 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W2P2P4|A0A2W2P2P4_9ACTN LLM class F420-dependent oxidoreductase OS=Streptomyces sp. NTH33 OX=1735453 GN=C1I97_09680 PE=4 SV=1
MM1 pKa = 7.51GVQQRR6 pKa = 11.84TSDD9 pKa = 3.27GGEE12 pKa = 3.9ALEE15 pKa = 4.11VWIDD19 pKa = 3.41QDD21 pKa = 3.99LCTGDD26 pKa = 5.01GICAQYY32 pKa = 10.9APEE35 pKa = 4.23VFEE38 pKa = 5.9LDD40 pKa = 3.05IDD42 pKa = 3.63GLAYY46 pKa = 10.42VKK48 pKa = 10.8SADD51 pKa = 4.87DD52 pKa = 4.5EE53 pKa = 4.46LLQDD57 pKa = 4.12SGATTPVPLPLLTDD71 pKa = 3.69VVDD74 pKa = 4.29SARR77 pKa = 11.84EE78 pKa = 3.94CPGEE82 pKa = 4.38CIHH85 pKa = 6.4VRR87 pKa = 11.84RR88 pKa = 11.84VSDD91 pKa = 3.27KK92 pKa = 10.85VEE94 pKa = 4.17VYY96 pKa = 10.9GPDD99 pKa = 3.36AEE101 pKa = 4.31

Molecular weight: 10.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W2LQM9|A0A2W2LQM9_9ACTN Uncharacterized protein OS=Streptomyces sp. NTH33 OX=1735453 GN=C1I97_13040 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7049

0

7049

2136631

20

6680

303.1

32.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.359 ± 0.04

0.834 ± 0.008

5.927 ± 0.024

5.833 ± 0.033

2.706 ± 0.016

9.368 ± 0.03

2.414 ± 0.015

3.098 ± 0.02

2.223 ± 0.021

10.317 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.733 ± 0.013

1.753 ± 0.017

6.038 ± 0.025

2.809 ± 0.015

8.462 ± 0.033

4.922 ± 0.019

6.041 ± 0.026

8.557 ± 0.029

1.514 ± 0.013

2.092 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski