Exiguobacterium phage vB_EalM-137

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3BWI3|A0A3G3BWI3_9CAUD Uncharacterized protein OS=Exiguobacterium phage vB_EalM-137 OX=2419624 GN=EalM137_00016 PE=4 SV=1
MM1 pKa = 7.99IYY3 pKa = 10.19QISSTVDD10 pKa = 3.05SEE12 pKa = 4.82PIDD15 pKa = 4.08FGATGVSAILQSVSFLLNSLVDD37 pKa = 3.75SHH39 pKa = 7.0PMHH42 pKa = 7.39RR43 pKa = 11.84DD44 pKa = 3.01FGMNPPIDD52 pKa = 4.73DD53 pKa = 3.96PCEE56 pKa = 3.55IARR59 pKa = 11.84NEE61 pKa = 3.53WSAQVIEE68 pKa = 4.56TIEE71 pKa = 4.06RR72 pKa = 11.84NIPEE76 pKa = 4.07VSVAEE81 pKa = 3.91VDD83 pKa = 4.29VIQDD87 pKa = 3.56EE88 pKa = 4.54SDD90 pKa = 3.51LLNGRR95 pKa = 11.84IKK97 pKa = 9.7TVVRR101 pKa = 11.84VVIEE105 pKa = 4.01VGG107 pKa = 3.09

Molecular weight: 11.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3BW23|A0A3G3BW23_9CAUD dUTPase OS=Exiguobacterium phage vB_EalM-137 OX=2419624 GN=EalM137_00029 PE=4 SV=1
MM1 pKa = 6.72TLEE4 pKa = 3.95RR5 pKa = 11.84ARR7 pKa = 11.84THH9 pKa = 7.01LEE11 pKa = 2.97AWYY14 pKa = 9.36QAEE17 pKa = 4.29IAVSTGQSYY26 pKa = 8.73TIGNRR31 pKa = 11.84TLTRR35 pKa = 11.84ANIKK39 pKa = 9.92DD40 pKa = 3.03IKK42 pKa = 9.71QQINYY47 pKa = 8.46WEE49 pKa = 4.11NRR51 pKa = 11.84VKK53 pKa = 11.02AMSGKK58 pKa = 9.43KK59 pKa = 9.05RR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84AARR65 pKa = 11.84IIPRR69 pKa = 11.84DD70 pKa = 3.49FF71 pKa = 4.74

Molecular weight: 8.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12587

43

1305

196.7

22.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.547 ± 0.391

0.413 ± 0.064

5.855 ± 0.248

7.746 ± 0.503

3.726 ± 0.191

7.087 ± 0.442

1.629 ± 0.143

6.451 ± 0.241

6.975 ± 0.365

8.326 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.781 ± 0.217

4.536 ± 0.248

3.631 ± 0.233

3.663 ± 0.197

5.657 ± 0.291

6.459 ± 0.309

5.776 ± 0.321

7.381 ± 0.226

1.287 ± 0.103

3.075 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski