Apis mellifera associated microvirus 32

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTX9|A0A3S8UTX9_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 32 OX=2494761 PE=3 SV=1
MM1 pKa = 7.57GFRR4 pKa = 11.84VNTAGRR10 pKa = 11.84VKK12 pKa = 10.23QSHH15 pKa = 6.79FSQVPANVAAPRR27 pKa = 11.84SPFDD31 pKa = 3.45RR32 pKa = 11.84SFSHH36 pKa = 6.23KK37 pKa = 7.25TTINEE42 pKa = 3.77GFLYY46 pKa = 9.99PIFWEE51 pKa = 5.08PILPGDD57 pKa = 4.02TVNLTMNCLARR68 pKa = 11.84LATPIFPYY76 pKa = 9.66MDD78 pKa = 3.37NVYY81 pKa = 10.93LDD83 pKa = 3.59VHH85 pKa = 6.11WFFVPNRR92 pKa = 11.84LVWNHH97 pKa = 5.56WEE99 pKa = 4.07QFQGAQDD106 pKa = 3.94NPPDD110 pKa = 3.6TFTDD114 pKa = 3.92YY115 pKa = 10.85EE116 pKa = 4.6VPSLDD121 pKa = 4.2DD122 pKa = 3.38ATHH125 pKa = 6.71AGGFASGSLYY135 pKa = 10.54DD136 pKa = 4.16YY137 pKa = 10.87FGLPTLVTGIDD148 pKa = 3.63QTNMPIALPFRR159 pKa = 11.84AYY161 pKa = 10.5RR162 pKa = 11.84KK163 pKa = 8.99IWNEE167 pKa = 3.41WYY169 pKa = 10.12RR170 pKa = 11.84DD171 pKa = 3.84EE172 pKa = 5.62NSQDD176 pKa = 3.59PLTVDD181 pKa = 3.52MGDD184 pKa = 4.06GPDD187 pKa = 3.41TTDD190 pKa = 3.08YY191 pKa = 11.72SLLKK195 pKa = 10.22RR196 pKa = 11.84NRR198 pKa = 11.84RR199 pKa = 11.84KK200 pKa = 10.66DD201 pKa = 3.53YY202 pKa = 8.17FTSCLPWPQKK212 pKa = 10.78GPAVTIPIGSGSLPVVLNPDD232 pKa = 5.51AIPDD236 pKa = 3.61SPIIRR241 pKa = 11.84RR242 pKa = 11.84ADD244 pKa = 3.49NQTSSQNSLTGLITSGAGALRR265 pKa = 11.84GSDD268 pKa = 3.42SFNSVIDD275 pKa = 4.14PNDD278 pKa = 3.42TLMVEE283 pKa = 4.59LDD285 pKa = 3.53DD286 pKa = 4.33VAAVSINTLRR296 pKa = 11.84EE297 pKa = 4.3SIVLQQMLEE306 pKa = 3.72LDD308 pKa = 3.49ARR310 pKa = 11.84GGTRR314 pKa = 11.84YY315 pKa = 9.91VEE317 pKa = 4.03ALLARR322 pKa = 11.84FGVVSPDD329 pKa = 3.21FRR331 pKa = 11.84LQRR334 pKa = 11.84PEE336 pKa = 3.8FLGGQTFDD344 pKa = 3.95INVSPIAQTSSTDD357 pKa = 3.1AEE359 pKa = 4.67TPQGNLSGFAVARR372 pKa = 11.84GSSQLSHH379 pKa = 7.06SFVEE383 pKa = 4.55HH384 pKa = 5.93GQLLGLVSVRR394 pKa = 11.84ADD396 pKa = 3.01ATYY399 pKa = 8.72QQGMSRR405 pKa = 11.84HH406 pKa = 4.32WSVRR410 pKa = 11.84TRR412 pKa = 11.84YY413 pKa = 10.14DD414 pKa = 3.16YY415 pKa = 11.52YY416 pKa = 11.22EE417 pKa = 4.36PLAANLGEE425 pKa = 3.93QAVLNKK431 pKa = 9.55EE432 pKa = 3.81IYY434 pKa = 7.36MTGTEE439 pKa = 4.1ATDD442 pKa = 3.38NAVFGYY448 pKa = 7.17QEE450 pKa = 3.24RR451 pKa = 11.84WAEE454 pKa = 3.85YY455 pKa = 9.34RR456 pKa = 11.84YY457 pKa = 10.22KK458 pKa = 10.43PSYY461 pKa = 7.85VTGIFRR467 pKa = 11.84SNAAASLDD475 pKa = 3.73SWHH478 pKa = 6.71LAIEE482 pKa = 4.76FGSLPTLADD491 pKa = 4.78LLPEE495 pKa = 4.45SPPIDD500 pKa = 4.15RR501 pKa = 11.84IVAVPSEE508 pKa = 3.85PHH510 pKa = 6.76FIVDD514 pKa = 3.15TWTKK518 pKa = 8.78FRR520 pKa = 11.84HH521 pKa = 4.95VRR523 pKa = 11.84VLPVYY528 pKa = 9.77SAPGLTRR535 pKa = 11.84LL536 pKa = 4.24

Molecular weight: 59.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTX9|A0A3S8UTX9_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 32 OX=2494761 PE=3 SV=1
MM1 pKa = 7.8PCYY4 pKa = 10.14KK5 pKa = 9.79PLKK8 pKa = 10.47AMVTRR13 pKa = 11.84SPSGGKK19 pKa = 8.08STVRR23 pKa = 11.84FHH25 pKa = 7.49KK26 pKa = 10.37GLKK29 pKa = 8.54LTACEE34 pKa = 4.36AKK36 pKa = 10.33HH37 pKa = 5.33GTATPIPCGQCVGCRR52 pKa = 11.84LEE54 pKa = 5.96RR55 pKa = 11.84SRR57 pKa = 11.84QWAIRR62 pKa = 11.84LMKK65 pKa = 9.83EE66 pKa = 4.05LKK68 pKa = 10.52LHH70 pKa = 6.53DD71 pKa = 4.08RR72 pKa = 11.84SSFLTLTYY80 pKa = 11.03DD81 pKa = 3.82DD82 pKa = 3.86AHH84 pKa = 6.94LPRR87 pKa = 11.84LRR89 pKa = 11.84NGLPTLVLEE98 pKa = 5.86DD99 pKa = 3.06IQLFLKK105 pKa = 10.58RR106 pKa = 11.84LRR108 pKa = 11.84KK109 pKa = 9.23EE110 pKa = 3.52LALPPEE116 pKa = 4.22SLRR119 pKa = 11.84YY120 pKa = 8.65FQAGEE125 pKa = 4.09YY126 pKa = 11.06GEE128 pKa = 4.54VTHH131 pKa = 6.97RR132 pKa = 11.84PHH134 pKa = 7.53HH135 pKa = 5.82HH136 pKa = 6.54MILFGEE142 pKa = 4.45DD143 pKa = 3.48FSRR146 pKa = 11.84DD147 pKa = 3.29RR148 pKa = 11.84VRR150 pKa = 11.84VADD153 pKa = 3.71SRR155 pKa = 11.84SGYY158 pKa = 10.65AQFEE162 pKa = 4.77SEE164 pKa = 5.12LLTSLWGKK172 pKa = 8.96GRR174 pKa = 11.84CTISEE179 pKa = 4.18VSFEE183 pKa = 4.05SAAYY187 pKa = 8.35VARR190 pKa = 11.84YY191 pKa = 8.74CLKK194 pKa = 10.36KK195 pKa = 9.87VTGKK199 pKa = 10.65GSRR202 pKa = 11.84FHH204 pKa = 5.93YY205 pKa = 9.94RR206 pKa = 11.84GRR208 pKa = 11.84KK209 pKa = 8.27PEE211 pKa = 3.95FVTMSRR217 pKa = 11.84RR218 pKa = 11.84PGIAARR224 pKa = 11.84YY225 pKa = 8.68FEE227 pKa = 4.61EE228 pKa = 4.82FKK230 pKa = 11.21SDD232 pKa = 3.4IYY234 pKa = 10.95PHH236 pKa = 7.15DD237 pKa = 3.92EE238 pKa = 4.13VVPGPGRR245 pKa = 11.84PASLPPKK252 pKa = 10.42YY253 pKa = 9.85FDD255 pKa = 4.2KK256 pKa = 11.06LLEE259 pKa = 4.43KK260 pKa = 10.49VDD262 pKa = 3.76PALFAEE268 pKa = 4.62IKK270 pKa = 9.53KK271 pKa = 10.49KK272 pKa = 10.42RR273 pKa = 11.84VEE275 pKa = 4.95GLDD278 pKa = 3.39FWSDD282 pKa = 3.52PNSTDD287 pKa = 2.67TRR289 pKa = 11.84LEE291 pKa = 3.55TRR293 pKa = 11.84EE294 pKa = 3.9RR295 pKa = 11.84VKK297 pKa = 10.98EE298 pKa = 3.96SLIKK302 pKa = 10.47NCLKK306 pKa = 10.78RR307 pKa = 11.84SVV309 pKa = 3.2

Molecular weight: 35.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1361

102

536

272.2

30.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.405 ± 1.88

0.808 ± 0.518

5.511 ± 0.609

5.731 ± 0.745

4.482 ± 0.926

6.98 ± 0.441

2.278 ± 0.315

3.6 ± 0.382

4.923 ± 1.42

8.891 ± 0.745

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.278 ± 0.492

3.821 ± 0.873

6.466 ± 1.117

3.527 ± 0.554

7.274 ± 0.803

8.597 ± 0.706

5.364 ± 0.792

6.025 ± 0.569

1.176 ± 0.416

2.866 ± 0.688
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski