Escherichia phage ID52

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Bullavirinae; Gequatrovirus; Escherichia virus ID52

Average proteome isoelectric point is 7.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2LM48|Q2LM48_9VIRU GpC OS=Escherichia phage ID52 OX=338108 GN=C PE=4 SV=1
MM1 pKa = 7.37SKK3 pKa = 10.67SNEE6 pKa = 4.12SAVAFQTAIASIKK19 pKa = 9.94LIQASSVLDD28 pKa = 3.62LTEE31 pKa = 5.86DD32 pKa = 3.73DD33 pKa = 5.33FDD35 pKa = 4.59FLTRR39 pKa = 11.84DD40 pKa = 3.8RR41 pKa = 11.84VWIATDD47 pKa = 3.49RR48 pKa = 11.84SRR50 pKa = 11.84ARR52 pKa = 11.84RR53 pKa = 11.84AIEE56 pKa = 3.8ACVYY60 pKa = 8.39GTLDD64 pKa = 3.27FVGYY68 pKa = 9.24PRR70 pKa = 11.84FPAPVEE76 pKa = 4.43FIAAVIAYY84 pKa = 7.7YY85 pKa = 8.79VHH87 pKa = 6.96PVNIQTACLIMEE99 pKa = 4.57GAEE102 pKa = 3.88FTEE105 pKa = 4.67NIVNGVEE112 pKa = 4.22RR113 pKa = 11.84PVKK116 pKa = 10.42ASEE119 pKa = 4.24LFAFTLLVRR128 pKa = 11.84AGNKK132 pKa = 9.77DD133 pKa = 3.97LIGHH137 pKa = 7.11AEE139 pKa = 4.04TNIRR143 pKa = 11.84EE144 pKa = 4.13QLRR147 pKa = 11.84AQGVMM152 pKa = 3.56

Molecular weight: 16.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2LM47|Q2LM47_9VIRU External scaffolding protein D OS=Escherichia phage ID52 OX=338108 GN=D PE=3 SV=1
MM1 pKa = 7.26EE2 pKa = 5.32RR3 pKa = 11.84WTLSGILAFLLLLSLLLPSLLIMFIPSTFRR33 pKa = 11.84RR34 pKa = 11.84PVLSWKK40 pKa = 9.79VQSLPKK46 pKa = 9.43TSLMVSNALLRR57 pKa = 11.84PPNCSPLLFSFVPEE71 pKa = 4.33TKK73 pKa = 9.75TLLVMPKK80 pKa = 8.14QTSVNNYY87 pKa = 9.14ALKK90 pKa = 10.14EE91 pKa = 3.95LCNEE95 pKa = 4.82KK96 pKa = 10.39INSPLRR102 pKa = 11.84RR103 pKa = 3.63

Molecular weight: 11.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

2379

25

554

216.3

24.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.86 ± 0.89

0.715 ± 0.137

5.338 ± 0.437

4.624 ± 0.481

3.867 ± 0.467

6.011 ± 0.654

2.354 ± 0.314

4.918 ± 0.427

6.389 ± 0.75

9.079 ± 1.107

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.111 ± 0.299

5.759 ± 0.372

4.288 ± 0.79

4.414 ± 0.691

5.464 ± 0.505

7.65 ± 0.545

7.104 ± 0.533

6.179 ± 0.806

1.387 ± 0.238

3.489 ± 0.428
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski