Verrucomicrobia bacterium SCGC AG-212-E04

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; unclassified Verrucomicrobia

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1004 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177Q5V4|A0A177Q5V4_9BACT Uncharacterized protein OS=Verrucomicrobia bacterium SCGC AG-212-E04 OX=1799654 GN=AYO41_00740 PE=4 SV=1
MM1 pKa = 7.3KK2 pKa = 10.21VAPLDD7 pKa = 3.99LPPVEE12 pKa = 5.19PGPEE16 pKa = 3.73PRR18 pKa = 11.84LEE20 pKa = 4.02EE21 pKa = 4.0MEE23 pKa = 4.57VEE25 pKa = 4.08IEE27 pKa = 3.98PVDD30 pKa = 3.99TIFSRR35 pKa = 11.84PFSNAMPPPPQMEE48 pKa = 4.2EE49 pKa = 3.89VLEE52 pKa = 4.65IDD54 pKa = 3.56ASSFGIQLPEE64 pKa = 3.72VQAPIEE70 pKa = 4.24PSAATQPSAGEE81 pKa = 4.08YY82 pKa = 10.06QDD84 pKa = 5.29LSQPLLVSPPPVPRR98 pKa = 11.84TAPVEE103 pKa = 4.36EE104 pKa = 4.98IDD106 pKa = 5.24PLDD109 pKa = 4.7LDD111 pKa = 5.1LVPLPDD117 pKa = 4.73LFCPKK122 pKa = 9.84CLSLIPEE129 pKa = 4.29SHH131 pKa = 6.61HH132 pKa = 5.5GSYY135 pKa = 8.8YY136 pKa = 9.23TEE138 pKa = 4.52CPKK141 pKa = 10.57CSWMIKK147 pKa = 9.16MKK149 pKa = 10.72

Molecular weight: 16.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177Q833|A0A177Q833_9BACT VOC domain-containing protein OS=Verrucomicrobia bacterium SCGC AG-212-E04 OX=1799654 GN=AYO41_03475 PE=4 SV=1
MM1 pKa = 7.36GRR3 pKa = 11.84TPPAISRR10 pKa = 11.84AALEE14 pKa = 3.93RR15 pKa = 11.84HH16 pKa = 5.5ISEE19 pKa = 5.12LGACRR24 pKa = 11.84RR25 pKa = 11.84CPRR28 pKa = 11.84MQPPVVTGGAVRR40 pKa = 11.84SRR42 pKa = 11.84ILHH45 pKa = 5.7VGQAPGDD52 pKa = 3.9KK53 pKa = 9.72EE54 pKa = 4.34PKK56 pKa = 9.58LGRR59 pKa = 11.84PFAWTAGRR67 pKa = 11.84TLFAWFHH74 pKa = 6.1EE75 pKa = 4.08ALGWSEE81 pKa = 3.51SDD83 pKa = 3.39YY84 pKa = 11.08RR85 pKa = 11.84SRR87 pKa = 11.84IYY89 pKa = 9.59MAAVCRR95 pKa = 11.84CFPGKK100 pKa = 10.36RR101 pKa = 11.84PGGGDD106 pKa = 3.21RR107 pKa = 11.84VPDD110 pKa = 4.19PGEE113 pKa = 3.89IANCASWLRR122 pKa = 11.84RR123 pKa = 11.84EE124 pKa = 4.03IEE126 pKa = 3.86ILRR129 pKa = 11.84PALVLPIGKK138 pKa = 9.05LAIAQFMEE146 pKa = 4.51VPRR149 pKa = 11.84LDD151 pKa = 3.72GLIGRR156 pKa = 11.84QFALEE161 pKa = 4.12KK162 pKa = 10.5FGHH165 pKa = 6.25CFDD168 pKa = 6.64LIPLPHH174 pKa = 7.35PSGASPWHH182 pKa = 5.94RR183 pKa = 11.84MEE185 pKa = 5.43PGRR188 pKa = 11.84TLLRR192 pKa = 11.84QALARR197 pKa = 11.84VAAHH201 pKa = 6.57PAVLSPVDD209 pKa = 3.22AARR212 pKa = 11.84CEE214 pKa = 4.07RR215 pKa = 11.84SRR217 pKa = 4.01

Molecular weight: 23.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1004

0

1004

319436

47

2558

318.2

34.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.696 ± 0.095

0.88 ± 0.023

5.051 ± 0.057

5.446 ± 0.084

4.309 ± 0.053

8.192 ± 0.079

1.994 ± 0.043

4.932 ± 0.052

3.558 ± 0.076

10.292 ± 0.105

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.081 ± 0.035

2.971 ± 0.051

5.663 ± 0.075

3.254 ± 0.044

7.124 ± 0.077

5.622 ± 0.071

5.47 ± 0.081

7.519 ± 0.061

1.474 ± 0.04

2.472 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski