Bacillus phage Belinda

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Wphvirus; unclassified Wphvirus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 293 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A192Y9H3|A0A192Y9H3_9CAUD Uncharacterized protein OS=Bacillus phage Belinda OX=1852564 GN=BELINDA_282 PE=4 SV=1
MM1 pKa = 7.36TNVNEE6 pKa = 4.13LKK8 pKa = 10.56AVEE11 pKa = 4.29EE12 pKa = 4.4KK13 pKa = 10.79EE14 pKa = 4.39VIQIEE19 pKa = 4.47SKK21 pKa = 10.58EE22 pKa = 4.29FNFPLYY28 pKa = 10.78DD29 pKa = 3.79GFDD32 pKa = 3.63EE33 pKa = 4.53EE34 pKa = 5.71LCDD37 pKa = 4.32SEE39 pKa = 4.76GNRR42 pKa = 11.84VTRR45 pKa = 11.84QALSFIVEE53 pKa = 4.48DD54 pKa = 4.05NQSGDD59 pKa = 3.64LDD61 pKa = 3.94RR62 pKa = 11.84VISDD66 pKa = 3.23MVDD69 pKa = 3.21RR70 pKa = 11.84YY71 pKa = 11.18SSDD74 pKa = 3.22STHH77 pKa = 7.54DD78 pKa = 2.97IWTMMRR84 pKa = 11.84DD85 pKa = 3.37ARR87 pKa = 11.84DD88 pKa = 3.46AFQDD92 pKa = 4.2AISEE96 pKa = 4.24GMVVVNPDD104 pKa = 3.54DD105 pKa = 4.22MNDD108 pKa = 3.43TMLNAYY114 pKa = 10.08LYY116 pKa = 10.94LLDD119 pKa = 6.09SEE121 pKa = 5.05LQANRR126 pKa = 11.84SEE128 pKa = 3.81ILYY131 pKa = 10.03NYY133 pKa = 9.44IVYY136 pKa = 9.99RR137 pKa = 11.84VNKK140 pKa = 8.91MLVDD144 pKa = 3.32KK145 pKa = 10.73HH146 pKa = 4.48VTQMDD151 pKa = 3.64IEE153 pKa = 4.3GLEE156 pKa = 3.98QEE158 pKa = 4.42IEE160 pKa = 4.2NYY162 pKa = 10.45ADD164 pKa = 3.74EE165 pKa = 5.46AEE167 pKa = 4.3SSYY170 pKa = 11.78SEE172 pKa = 4.24FDD174 pKa = 3.62YY175 pKa = 11.03MDD177 pKa = 5.55DD178 pKa = 3.55EE179 pKa = 4.61MFSQLEE185 pKa = 4.16EE186 pKa = 4.99FIDD189 pKa = 4.02SYY191 pKa = 11.34IAGLEE196 pKa = 3.86MGQFLDD202 pKa = 3.67NQDD205 pKa = 3.14WEE207 pKa = 4.64NIGDD211 pKa = 3.7VTITEE216 pKa = 3.99GAMFIKK222 pKa = 10.28KK223 pKa = 10.42DD224 pKa = 3.43EE225 pKa = 4.53DD226 pKa = 3.87TDD228 pKa = 3.16NSYY231 pKa = 11.25YY232 pKa = 9.96VEE234 pKa = 3.84RR235 pKa = 11.84VSGNEE240 pKa = 3.67FEE242 pKa = 5.7SFYY245 pKa = 10.6LDD247 pKa = 4.3SMYY250 pKa = 10.68IDD252 pKa = 5.97LNDD255 pKa = 3.85GWINWYY261 pKa = 9.95DD262 pKa = 3.23INEE265 pKa = 4.14YY266 pKa = 11.02AGISMDD272 pKa = 3.37AEE274 pKa = 4.28PYY276 pKa = 10.18EE277 pKa = 4.9RR278 pKa = 11.84ISALIGYY285 pKa = 9.58DD286 pKa = 3.29GIEE289 pKa = 4.24NFNPSTTEE297 pKa = 3.67CKK299 pKa = 9.39TAIDD303 pKa = 3.46LCGALRR309 pKa = 11.84NAGVNVVWSTEE320 pKa = 3.76HH321 pKa = 7.43EE322 pKa = 4.34EE323 pKa = 4.03IQFF326 pKa = 3.63

Molecular weight: 37.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A192YA25|A0A192YA25_9CAUD Tap measure protein OS=Bacillus phage Belinda OX=1852564 GN=BELINDA_104 PE=4 SV=1
MM1 pKa = 7.27ARR3 pKa = 11.84KK4 pKa = 8.54PRR6 pKa = 11.84KK7 pKa = 8.19PRR9 pKa = 11.84KK10 pKa = 9.12VPKK13 pKa = 10.04LLRR16 pKa = 11.84GGQDD20 pKa = 2.81TSRR23 pKa = 11.84ALNNIGKK30 pKa = 9.56ALQQQALQSTAKK42 pKa = 9.9QLSEE46 pKa = 4.32SLPEE50 pKa = 4.47GYY52 pKa = 9.52EE53 pKa = 3.75LKK55 pKa = 10.39QRR57 pKa = 11.84PKK59 pKa = 10.12YY60 pKa = 10.98LEE62 pKa = 3.91VTEE65 pKa = 4.42KK66 pKa = 10.77RR67 pKa = 11.84LIGMGVVDD75 pKa = 6.2LKK77 pKa = 11.45PMFAKK82 pKa = 10.34SPKK85 pKa = 9.95RR86 pKa = 11.84KK87 pKa = 9.08FSKK90 pKa = 10.73NGGWYY95 pKa = 9.79LDD97 pKa = 3.39VPIRR101 pKa = 11.84RR102 pKa = 11.84KK103 pKa = 10.05ARR105 pKa = 11.84GMSRR109 pKa = 11.84RR110 pKa = 11.84MYY112 pKa = 10.11DD113 pKa = 2.95QLRR116 pKa = 11.84AVDD119 pKa = 3.91MGGQQKK125 pKa = 10.4INIISDD131 pKa = 3.46YY132 pKa = 10.78LYY134 pKa = 10.86DD135 pKa = 3.68NRR137 pKa = 11.84GVSDD141 pKa = 4.72APLLNYY147 pKa = 9.49KK148 pKa = 9.88PKK150 pKa = 10.92SNTITKK156 pKa = 9.47IASGKK161 pKa = 9.42GRR163 pKa = 11.84HH164 pKa = 6.64DD165 pKa = 3.29YY166 pKa = 10.67VAFRR170 pKa = 11.84RR171 pKa = 11.84VSNNSSPTSWIVNRR185 pKa = 11.84DD186 pKa = 3.18KK187 pKa = 11.16VTVANTSKK195 pKa = 9.8TFVANVNRR203 pKa = 11.84LMKK206 pKa = 10.94YY207 pKa = 9.52NMKK210 pKa = 10.75NMM212 pKa = 3.89

Molecular weight: 24.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

293

0

293

49433

22

2005

168.7

19.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.105 ± 0.231

0.904 ± 0.077

6.591 ± 0.131

8.213 ± 0.319

4.03 ± 0.098

6.099 ± 0.272

1.855 ± 0.108

6.56 ± 0.147

7.833 ± 0.207

7.881 ± 0.16

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.152 ± 0.101

5.43 ± 0.161

2.788 ± 0.147

3.245 ± 0.132

4.493 ± 0.121

5.537 ± 0.135

6.439 ± 0.237

7.234 ± 0.155

1.155 ± 0.062

4.457 ± 0.123
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski