Lactobacillus phage Lenus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Tybeckvirinae; Lenusvirus; Lactobacillus virus Lenus

Average proteome isoelectric point is 5.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 131 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4PB94|A0A2H4PB94_9CAUD Uncharacterized protein OS=Lactobacillus phage Lenus OX=2053682 PE=4 SV=1
MM1 pKa = 7.94EE2 pKa = 5.41DD3 pKa = 3.75TDD5 pKa = 3.66EE6 pKa = 4.32MLYY9 pKa = 10.36QATKK13 pKa = 10.02AANGFDD19 pKa = 3.14WTFDD23 pKa = 3.02LRR25 pKa = 11.84AAIEE29 pKa = 4.58YY30 pKa = 8.18YY31 pKa = 9.86TDD33 pKa = 3.23VYY35 pKa = 8.44MTSYY39 pKa = 11.54DD40 pKa = 3.65EE41 pKa = 5.07AIKK44 pKa = 10.7NGKK47 pKa = 6.7TEE49 pKa = 4.15EE50 pKa = 4.29EE51 pKa = 4.34SSQVARR57 pKa = 11.84NVLITMMEE65 pKa = 3.94YY66 pKa = 10.95GDD68 pKa = 4.61QINGDD73 pKa = 3.47TDD75 pKa = 3.43EE76 pKa = 4.35

Molecular weight: 8.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4PB68|A0A2H4PB68_9CAUD Uncharacterized protein OS=Lactobacillus phage Lenus OX=2053682 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.16FYY4 pKa = 10.98KK5 pKa = 10.65LLIAVVAGVTLSLVTAYY22 pKa = 10.81NADD25 pKa = 3.53ASKK28 pKa = 10.66KK29 pKa = 9.24PNYY32 pKa = 9.85AYY34 pKa = 11.07SLTRR38 pKa = 11.84TSQHH42 pKa = 6.67AIKK45 pKa = 9.25LTNTGRR51 pKa = 11.84TEE53 pKa = 3.42NMYY56 pKa = 10.57RR57 pKa = 11.84VTVKK61 pKa = 10.65SNKK64 pKa = 5.8TTWYY68 pKa = 10.63YY69 pKa = 9.8MALNSRR75 pKa = 11.84QSWMIKK81 pKa = 9.88QPGKK85 pKa = 10.22YY86 pKa = 8.53SVTVRR91 pKa = 11.84RR92 pKa = 11.84VSKK95 pKa = 10.81SDD97 pKa = 3.2EE98 pKa = 4.01KK99 pKa = 11.29RR100 pKa = 11.84NADD103 pKa = 3.67PRR105 pKa = 11.84NHH107 pKa = 7.39FIPQGIKK114 pKa = 8.56NTQRR118 pKa = 11.84TIWNRR123 pKa = 3.1

Molecular weight: 14.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

131

0

131

23326

30

1886

178.1

20.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.878 ± 0.492

0.806 ± 0.105

7.387 ± 0.391

6.023 ± 0.323

3.94 ± 0.172

7.082 ± 0.415

1.663 ± 0.126

7.061 ± 0.214

8.158 ± 0.351

7.738 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.107

6.388 ± 0.197

2.649 ± 0.149

3.335 ± 0.158

3.443 ± 0.167

7.309 ± 0.319

5.903 ± 0.353

6.482 ± 0.216

1.333 ± 0.11

4.651 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski