Pseudonocardia endophytica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Pseudonocardia

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6902 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V2PHI9|A0A4V2PHI9_9PSEU Putative colanic acid biosynthesis acetyltransferase WcaF OS=Pseudonocardia endophytica OX=401976 GN=EV378_4904 PE=4 SV=1
MM1 pKa = 7.74SPEE4 pKa = 4.45GLPPDD9 pKa = 4.85PFAGDD14 pKa = 3.65PDD16 pKa = 4.85DD17 pKa = 5.33PALCMDD23 pKa = 5.53PGSEE27 pKa = 3.8GRR29 pKa = 11.84TDD31 pKa = 4.12GEE33 pKa = 4.28DD34 pKa = 4.18AEE36 pKa = 5.0LTDD39 pKa = 3.92SDD41 pKa = 4.17RR42 pKa = 11.84SEE44 pKa = 4.14ILADD48 pKa = 3.78LADD51 pKa = 4.03LAVYY55 pKa = 9.68QALLSGRR62 pKa = 11.84GVRR65 pKa = 11.84GIVVDD70 pKa = 4.07CTDD73 pKa = 3.41CGEE76 pKa = 4.19QHH78 pKa = 4.99FHH80 pKa = 5.74EE81 pKa = 4.93WSLLRR86 pKa = 11.84ASLQQLLDD94 pKa = 3.76EE95 pKa = 4.95GQMRR99 pKa = 11.84PHH101 pKa = 6.78EE102 pKa = 4.48PAYY105 pKa = 10.75DD106 pKa = 4.26PDD108 pKa = 4.08PAHH111 pKa = 5.96YY112 pKa = 8.56VTWEE116 pKa = 3.85YY117 pKa = 11.22CRR119 pKa = 11.84GYY121 pKa = 11.45ADD123 pKa = 4.01ATLADD128 pKa = 4.3DD129 pKa = 4.09

Molecular weight: 14.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R1HRD1|A0A4R1HRD1_9PSEU Cytochrome bd-I ubiquinol oxidase subunit 2 apoprotein OS=Pseudonocardia endophytica OX=401976 GN=EV378_6341 PE=3 SV=1
MM1 pKa = 7.19MIKK4 pKa = 10.15SAVLAATSVRR14 pKa = 11.84SHH16 pKa = 4.89GRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84TRR22 pKa = 11.84TRR24 pKa = 11.84SRR26 pKa = 11.84TRR28 pKa = 11.84SRR30 pKa = 11.84GG31 pKa = 3.3

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6902

0

6902

2267065

29

10989

328.5

34.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.562 ± 0.049

0.751 ± 0.008

6.821 ± 0.03

5.402 ± 0.028

2.666 ± 0.017

9.838 ± 0.033

2.219 ± 0.013

3.113 ± 0.019

1.399 ± 0.02

9.986 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.749 ± 0.012

1.571 ± 0.014

6.272 ± 0.026

2.397 ± 0.018

8.38 ± 0.034

5.107 ± 0.022

6.071 ± 0.02

9.437 ± 0.027

1.461 ± 0.012

1.797 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski