Marvinbryantia formatexigens DSM 14469

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Marvinbryantia; Marvinbryantia formatexigens

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4894 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6LB72|C6LB72_9FIRM ABC transporter substrate-binding protein family 5 OS=Marvinbryantia formatexigens DSM 14469 OX=478749 GN=BRYFOR_05867 PE=4 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84KK3 pKa = 9.94SSMKK7 pKa = 8.89ITGAVLCAAMCMSMTGGVSAEE28 pKa = 3.96EE29 pKa = 4.38TIEE32 pKa = 3.89VAYY35 pKa = 11.05NLTTEE40 pKa = 4.25DD41 pKa = 3.58FAVFEE46 pKa = 4.57QITKK50 pKa = 10.79DD51 pKa = 3.43FTEE54 pKa = 4.08EE55 pKa = 3.51TGIGVTIYY63 pKa = 10.99NGGDD67 pKa = 3.5DD68 pKa = 4.51YY69 pKa = 11.76EE70 pKa = 4.83SAMKK74 pKa = 10.03TRR76 pKa = 11.84MSSGDD81 pKa = 4.21LPDD84 pKa = 2.76MWTTHH89 pKa = 4.54GWSLIRR95 pKa = 11.84YY96 pKa = 8.23SEE98 pKa = 3.99YY99 pKa = 11.04MMDD102 pKa = 5.12LSDD105 pKa = 4.77QEE107 pKa = 4.35WVSNIDD113 pKa = 3.38EE114 pKa = 4.33GLKK117 pKa = 10.66NVITNDD123 pKa = 3.51DD124 pKa = 4.0GEE126 pKa = 4.92LFILPITQAVTAIVYY141 pKa = 10.39NKK143 pKa = 10.46DD144 pKa = 3.37VLDD147 pKa = 3.99EE148 pKa = 4.92AGVDD152 pKa = 3.58ASAIRR157 pKa = 11.84TWDD160 pKa = 3.63DD161 pKa = 3.11FDD163 pKa = 5.26AALQAVADD171 pKa = 4.06NTEE174 pKa = 3.95ATPLEE179 pKa = 4.17ICMGDD184 pKa = 3.54GYY186 pKa = 11.16DD187 pKa = 3.84SYY189 pKa = 11.55SLEE192 pKa = 5.15DD193 pKa = 3.41IWPTLYY199 pKa = 10.67TNSDD203 pKa = 3.48LADD206 pKa = 3.69NKK208 pKa = 11.24AEE210 pKa = 4.09TLQDD214 pKa = 3.52GTFDD218 pKa = 3.28WDD220 pKa = 3.33ADD222 pKa = 3.6GRR224 pKa = 11.84EE225 pKa = 4.35GFQMLADD232 pKa = 4.73WYY234 pKa = 9.15TKK236 pKa = 10.52GYY238 pKa = 9.3MNEE241 pKa = 4.61DD242 pKa = 3.64YY243 pKa = 11.52VSGKK247 pKa = 10.23KK248 pKa = 10.07DD249 pKa = 4.26DD250 pKa = 3.6ICKK253 pKa = 10.61AMANNEE259 pKa = 4.24GAFTLYY265 pKa = 8.39STEE268 pKa = 4.38MIPSVLAYY276 pKa = 10.69NEE278 pKa = 3.95NANLGILPVPSKK290 pKa = 10.46EE291 pKa = 4.12ADD293 pKa = 3.23GASYY297 pKa = 10.6FGTGEE302 pKa = 4.37GNFSCFGIWKK312 pKa = 8.87DD313 pKa = 3.8TEE315 pKa = 4.79HH316 pKa = 7.37EE317 pKa = 4.22DD318 pKa = 3.57ACKK321 pKa = 10.41QFLSYY326 pKa = 10.66LARR329 pKa = 11.84PEE331 pKa = 4.02VAVKK335 pKa = 10.26IVQIDD340 pKa = 3.52GGIPALTNIEE350 pKa = 4.45LDD352 pKa = 3.71EE353 pKa = 4.51TDD355 pKa = 3.13QAAYY359 pKa = 5.66TTNAFRR365 pKa = 11.84EE366 pKa = 4.32AQEE369 pKa = 4.14QFDD372 pKa = 4.18GDD374 pKa = 4.66LIYY377 pKa = 11.12DD378 pKa = 3.52NFFDD382 pKa = 5.47RR383 pKa = 11.84EE384 pKa = 4.15YY385 pKa = 10.24LPSGMWSVMSDD396 pKa = 3.05AVQVLLSDD404 pKa = 4.7GDD406 pKa = 4.19PEE408 pKa = 6.05ADD410 pKa = 3.11MDD412 pKa = 4.23EE413 pKa = 4.45AVMMVADD420 pKa = 4.82NYY422 pKa = 10.93NDD424 pKa = 3.81LMGNN428 pKa = 3.81

Molecular weight: 47.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6LKL5|C6LKL5_9FIRM Uncharacterized protein OS=Marvinbryantia formatexigens DSM 14469 OX=478749 GN=BRYFOR_09203 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.29GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.5TAGGRR28 pKa = 11.84KK29 pKa = 8.77VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.75GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4894

0

4894

1389242

36

2109

283.9

31.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.243 ± 0.047

1.621 ± 0.017

5.329 ± 0.031

7.72 ± 0.044

4.102 ± 0.028

7.316 ± 0.037

1.699 ± 0.017

6.783 ± 0.034

6.059 ± 0.031

8.995 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.145 ± 0.019

3.951 ± 0.023

3.389 ± 0.022

3.429 ± 0.022

5.06 ± 0.038

5.682 ± 0.029

5.544 ± 0.038

6.713 ± 0.027

1.046 ± 0.012

4.173 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski