Serratia symbiotica str. Cinara cedri

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Yersiniaceae; Serratia; Serratia symbiotica

Average proteome isoelectric point is 7.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 672 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4I1F0|R4I1F0_9GAMM GMP synthase [glutamine-hydrolyzing] OS=Serratia symbiotica str. 'Cinara cedri' OX=568817 GN=guaA PE=3 SV=1
MM1 pKa = 8.17DD2 pKa = 4.78SLIVPDD8 pKa = 5.43LSLLRR13 pKa = 11.84HH14 pKa = 6.04WLDD17 pKa = 3.12QSGISFFEE25 pKa = 4.71CNACPVLHH33 pKa = 6.77LPHH36 pKa = 6.2MQSFAGVCDD45 pKa = 3.94AKK47 pKa = 11.21VDD49 pKa = 4.15LVDD52 pKa = 3.78NVILFSVLAEE62 pKa = 4.19VKK64 pKa = 8.7PTALIPLVADD74 pKa = 4.48LSLINASSLTIKK86 pKa = 10.72AFVDD90 pKa = 3.47IQDD93 pKa = 4.16DD94 pKa = 4.16NLPKK98 pKa = 10.75LIVCQSLSIAVGVTLKK114 pKa = 10.69QFTHH118 pKa = 7.0FMQQSEE124 pKa = 4.39EE125 pKa = 4.1QISLVILEE133 pKa = 4.48ARR135 pKa = 11.84ANDD138 pKa = 4.14LLFIDD143 pKa = 5.43DD144 pKa = 4.36EE145 pKa = 4.75EE146 pKa = 4.79EE147 pKa = 3.91NSSSIEE153 pKa = 4.08CQPVLHH159 pKa = 6.82

Molecular weight: 17.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4I1Q2|R4I1Q2_9GAMM FAD-dependent pyridine nucleotide-disulfide oxidoreductase OS=Serratia symbiotica str. 'Cinara cedri' OX=568817 GN=ndh PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.53RR3 pKa = 11.84TFQPSILKK11 pKa = 10.32RR12 pKa = 11.84NRR14 pKa = 11.84NHH16 pKa = 6.87GFRR19 pKa = 11.84ARR21 pKa = 11.84MANKK25 pKa = 9.74NGRR28 pKa = 11.84QILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.47GRR39 pKa = 11.84TRR41 pKa = 11.84LTVSKK46 pKa = 10.95

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

672

0

672

227463

38

1408

338.5

37.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.556 ± 0.083

1.29 ± 0.032

4.727 ± 0.065

5.181 ± 0.08

3.731 ± 0.06

6.643 ± 0.069

2.548 ± 0.045

8.721 ± 0.094

5.538 ± 0.074

10.833 ± 0.096

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.642 ± 0.043

4.912 ± 0.068

3.697 ± 0.039

4.215 ± 0.058

5.294 ± 0.07

6.209 ± 0.065

5.265 ± 0.05

6.514 ± 0.075

1.115 ± 0.034

3.368 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski