Amino acid dipepetide frequency for Nitrosomonas nitrosa

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.355AlaAla: 9.355 ± 0.136
1.099AlaCys: 1.099 ± 0.037
4.679AlaAsp: 4.679 ± 0.072
5.718AlaGlu: 5.718 ± 0.08
3.522AlaPhe: 3.522 ± 0.068
6.544AlaGly: 6.544 ± 0.102
2.266AlaHis: 2.266 ± 0.047
6.645AlaIle: 6.645 ± 0.099
4.015AlaLys: 4.015 ± 0.072
10.46AlaLeu: 10.46 ± 0.124
2.378AlaMet: 2.378 ± 0.053
3.346AlaAsn: 3.346 ± 0.071
3.173AlaPro: 3.173 ± 0.068
3.803AlaGln: 3.803 ± 0.066
5.331AlaArg: 5.331 ± 0.08
5.217AlaSer: 5.217 ± 0.08
4.504AlaThr: 4.504 ± 0.075
6.05AlaVal: 6.05 ± 0.101
1.219AlaTrp: 1.219 ± 0.035
2.581AlaTyr: 2.581 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.881CysAla: 0.881 ± 0.032
0.155CysCys: 0.155 ± 0.014
0.542CysAsp: 0.542 ± 0.026
0.541CysGlu: 0.541 ± 0.026
0.45CysPhe: 0.45 ± 0.024
0.913CysGly: 0.913 ± 0.037
0.39CysHis: 0.39 ± 0.025
0.603CysIle: 0.603 ± 0.028
0.386CysLys: 0.386 ± 0.024
0.943CysLeu: 0.943 ± 0.029
0.214CysMet: 0.214 ± 0.014
0.366CysAsn: 0.366 ± 0.022
0.493CysPro: 0.493 ± 0.029
0.38CysGln: 0.38 ± 0.019
0.581CysArg: 0.581 ± 0.03
0.588CysSer: 0.588 ± 0.028
0.47CysThr: 0.47 ± 0.028
0.644CysVal: 0.644 ± 0.027
0.153CysTrp: 0.153 ± 0.015
0.34CysTyr: 0.34 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
4.59AspAla: 4.59 ± 0.075
0.502AspCys: 0.502 ± 0.022
2.63AspAsp: 2.63 ± 0.069
3.549AspGlu: 3.549 ± 0.067
2.412AspPhe: 2.412 ± 0.062
3.39AspGly: 3.39 ± 0.073
1.361AspHis: 1.361 ± 0.039
3.687AspIle: 3.687 ± 0.073
2.459AspLys: 2.459 ± 0.068
5.186AspLeu: 5.186 ± 0.085
1.189AspMet: 1.189 ± 0.039
1.86AspAsn: 1.86 ± 0.059
2.621AspPro: 2.621 ± 0.065
2.162AspGln: 2.162 ± 0.048
3.057AspArg: 3.057 ± 0.07
2.771AspSer: 2.771 ± 0.062
2.753AspThr: 2.753 ± 0.076
3.171AspVal: 3.171 ± 0.075
0.882AspTrp: 0.882 ± 0.034
1.929AspTyr: 1.929 ± 0.056
0.0AspXaa: 0.0 ± 0.0
Glu
5.678GluAla: 5.678 ± 0.083
0.469GluCys: 0.469 ± 0.025
2.595GluAsp: 2.595 ± 0.058
3.732GluGlu: 3.732 ± 0.068
2.143GluPhe: 2.143 ± 0.053
3.602GluGly: 3.602 ± 0.066
1.49GluHis: 1.49 ± 0.042
4.933GluIle: 4.933 ± 0.071
3.493GluLys: 3.493 ± 0.073
6.248GluLeu: 6.248 ± 0.082
1.655GluMet: 1.655 ± 0.051
2.483GluAsn: 2.483 ± 0.059
2.072GluPro: 2.072 ± 0.053
3.14GluGln: 3.14 ± 0.068
4.134GluArg: 4.134 ± 0.08
3.457GluSer: 3.457 ± 0.055
3.164GluThr: 3.164 ± 0.066
3.804GluVal: 3.804 ± 0.072
0.849GluTrp: 0.849 ± 0.031
1.548GluTyr: 1.548 ± 0.042
0.0GluXaa: 0.0 ± 0.0
Phe
3.517PheAla: 3.517 ± 0.069
0.478PheCys: 0.478 ± 0.023
2.574PheAsp: 2.574 ± 0.056
2.361PheGlu: 2.361 ± 0.056
1.956PhePhe: 1.956 ± 0.052
3.078PheGly: 3.078 ± 0.064
0.974PheHis: 0.974 ± 0.035
2.664PheIle: 2.664 ± 0.057
1.529PheLys: 1.529 ± 0.048
3.835PheLeu: 3.835 ± 0.074
0.959PheMet: 0.959 ± 0.039
1.643PheAsn: 1.643 ± 0.048
1.75PhePro: 1.75 ± 0.044
1.364PheGln: 1.364 ± 0.037
2.045PheArg: 2.045 ± 0.052
2.952PheSer: 2.952 ± 0.067
2.241PheThr: 2.241 ± 0.053
2.634PheVal: 2.634 ± 0.062
0.572PheTrp: 0.572 ± 0.029
1.342PheTyr: 1.342 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
5.497GlyAla: 5.497 ± 0.094
0.871GlyCys: 0.871 ± 0.034
3.378GlyAsp: 3.378 ± 0.071
4.157GlyGlu: 4.157 ± 0.071
3.007GlyPhe: 3.007 ± 0.061
5.097GlyGly: 5.097 ± 0.118
1.722GlyHis: 1.722 ± 0.05
5.166GlyIle: 5.166 ± 0.089
3.963GlyLys: 3.963 ± 0.074
7.114GlyLeu: 7.114 ± 0.082
2.084GlyMet: 2.084 ± 0.05
2.551GlyAsn: 2.551 ± 0.071
1.887GlyPro: 1.887 ± 0.054
2.715GlyGln: 2.715 ± 0.051
3.902GlyArg: 3.902 ± 0.067
4.025GlySer: 4.025 ± 0.076
3.628GlyThr: 3.628 ± 0.078
4.905GlyVal: 4.905 ± 0.086
1.071GlyTrp: 1.071 ± 0.036
2.436GlyTyr: 2.436 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
2.427HisAla: 2.427 ± 0.053
0.332HisCys: 0.332 ± 0.017
1.353HisAsp: 1.353 ± 0.034
1.439HisGlu: 1.439 ± 0.042
1.21HisPhe: 1.21 ± 0.038
1.91HisGly: 1.91 ± 0.05
0.941HisHis: 0.941 ± 0.04
1.61HisIle: 1.61 ± 0.045
0.93HisLys: 0.93 ± 0.03
2.639HisLeu: 2.639 ± 0.048
0.485HisMet: 0.485 ± 0.022
0.871HisAsn: 0.871 ± 0.034
1.62HisPro: 1.62 ± 0.038
1.059HisGln: 1.059 ± 0.034
1.504HisArg: 1.504 ± 0.045
1.32HisSer: 1.32 ± 0.034
1.295HisThr: 1.295 ± 0.036
1.568HisVal: 1.568 ± 0.034
0.392HisTrp: 0.392 ± 0.019
0.98HisTyr: 0.98 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
6.979IleAla: 6.979 ± 0.093
0.696IleCys: 0.696 ± 0.029
4.238IleAsp: 4.238 ± 0.076
4.897IleGlu: 4.897 ± 0.074
2.514IlePhe: 2.514 ± 0.065
4.902IleGly: 4.902 ± 0.079
1.666IleHis: 1.666 ± 0.043
3.972IleIle: 3.972 ± 0.066
3.178IleLys: 3.178 ± 0.058
6.357IleLeu: 6.357 ± 0.109
1.417IleMet: 1.417 ± 0.042
2.717IleAsn: 2.717 ± 0.055
3.294IlePro: 3.294 ± 0.056
2.452IleGln: 2.452 ± 0.056
3.615IleArg: 3.615 ± 0.066
4.194IleSer: 4.194 ± 0.07
3.843IleThr: 3.843 ± 0.064
4.553IleVal: 4.553 ± 0.076
0.761IleTrp: 0.761 ± 0.031
1.79IleTyr: 1.79 ± 0.041
0.0IleXaa: 0.0 ± 0.0
Lys
3.871LysAla: 3.871 ± 0.07
0.297LysCys: 0.297 ± 0.015
1.987LysAsp: 1.987 ± 0.056
2.784LysGlu: 2.784 ± 0.068
1.387LysPhe: 1.387 ± 0.037
2.619LysGly: 2.619 ± 0.056
1.111LysHis: 1.111 ± 0.036
3.369LysIle: 3.369 ± 0.073
2.439LysLys: 2.439 ± 0.056
5.032LysLeu: 5.032 ± 0.082
1.206LysMet: 1.206 ± 0.042
1.968LysAsn: 1.968 ± 0.053
2.151LysPro: 2.151 ± 0.059
2.438LysGln: 2.438 ± 0.054
2.937LysArg: 2.937 ± 0.055
2.557LysSer: 2.557 ± 0.07
2.635LysThr: 2.635 ± 0.051
2.761LysVal: 2.761 ± 0.061
0.536LysTrp: 0.536 ± 0.026
1.12LysTyr: 1.12 ± 0.033
0.0LysXaa: 0.0 ± 0.0
Leu
10.845LeuAla: 10.845 ± 0.143
1.052LeuCys: 1.052 ± 0.037
5.77LeuAsp: 5.77 ± 0.085
6.13LeuGlu: 6.13 ± 0.1
4.286LeuPhe: 4.286 ± 0.071
7.078LeuGly: 7.078 ± 0.1
2.591LeuHis: 2.591 ± 0.055
6.876LeuIle: 6.876 ± 0.111
4.806LeuLys: 4.806 ± 0.076
11.992LeuLeu: 11.992 ± 0.174
2.436LeuMet: 2.436 ± 0.049
3.887LeuAsn: 3.887 ± 0.061
5.819LeuPro: 5.819 ± 0.099
4.285LeuGln: 4.285 ± 0.076
6.293LeuArg: 6.293 ± 0.091
7.208LeuSer: 7.208 ± 0.095
6.174LeuThr: 6.174 ± 0.086
6.74LeuVal: 6.74 ± 0.103
1.225LeuTrp: 1.225 ± 0.047
2.678LeuTyr: 2.678 ± 0.058
0.0LeuXaa: 0.0 ± 0.0
Met
2.328MetAla: 2.328 ± 0.055
0.166MetCys: 0.166 ± 0.013
1.087MetAsp: 1.087 ± 0.035
1.288MetGlu: 1.288 ± 0.035
0.709MetPhe: 0.709 ± 0.029
1.625MetGly: 1.625 ± 0.043
0.63MetHis: 0.63 ± 0.029
1.554MetIle: 1.554 ± 0.047
1.211MetLys: 1.211 ± 0.036
2.799MetLeu: 2.799 ± 0.054
0.613MetMet: 0.613 ± 0.031
1.095MetAsn: 1.095 ± 0.034
1.326MetPro: 1.326 ± 0.038
1.139MetGln: 1.139 ± 0.036
1.59MetArg: 1.59 ± 0.045
1.518MetSer: 1.518 ± 0.041
1.495MetThr: 1.495 ± 0.041
1.598MetVal: 1.598 ± 0.047
0.171MetTrp: 0.171 ± 0.013
0.381MetTyr: 0.381 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
3.204AsnAla: 3.204 ± 0.066
0.372AsnCys: 0.372 ± 0.019
2.004AsnAsp: 2.004 ± 0.061
2.218AsnGlu: 2.218 ± 0.045
1.434AsnPhe: 1.434 ± 0.043
2.601AsnGly: 2.601 ± 0.058
0.961AsnHis: 0.961 ± 0.03
2.447AsnIle: 2.447 ± 0.052
1.714AsnLys: 1.714 ± 0.049
3.948AsnLeu: 3.948 ± 0.065
0.824AsnMet: 0.824 ± 0.03
1.545AsnAsn: 1.545 ± 0.05
2.185AsnPro: 2.185 ± 0.051
1.817AsnGln: 1.817 ± 0.053
2.284AsnArg: 2.284 ± 0.052
2.017AsnSer: 2.017 ± 0.072
1.924AsnThr: 1.924 ± 0.055
2.172AsnVal: 2.172 ± 0.055
0.603AsnTrp: 0.603 ± 0.029
1.116AsnTyr: 1.116 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
4.001ProAla: 4.001 ± 0.08
0.392ProCys: 0.392 ± 0.021
2.958ProAsp: 2.958 ± 0.06
3.275ProGlu: 3.275 ± 0.063
1.949ProPhe: 1.949 ± 0.046
3.278ProGly: 3.278 ± 0.061
1.182ProHis: 1.182 ± 0.034
2.842ProIle: 2.842 ± 0.056
1.75ProLys: 1.75 ± 0.05
4.668ProLeu: 4.668 ± 0.086
1.006ProMet: 1.006 ± 0.033
1.604ProAsn: 1.604 ± 0.045
2.151ProPro: 2.151 ± 0.064
1.831ProGln: 1.831 ± 0.047
2.082ProArg: 2.082 ± 0.05
2.628ProSer: 2.628 ± 0.057
2.256ProThr: 2.256 ± 0.054
3.485ProVal: 3.485 ± 0.057
0.58ProTrp: 0.58 ± 0.029
1.346ProTyr: 1.346 ± 0.048
0.0ProXaa: 0.0 ± 0.0
Gln
4.398GlnAla: 4.398 ± 0.072
0.364GlnCys: 0.364 ± 0.022
1.844GlnAsp: 1.844 ± 0.045
2.381GlnGlu: 2.381 ± 0.058
1.643GlnPhe: 1.643 ± 0.04
2.785GlnGly: 2.785 ± 0.049
1.268GlnHis: 1.268 ± 0.039
2.899GlnIle: 2.899 ± 0.055
1.878GlnLys: 1.878 ± 0.048
4.789GlnLeu: 4.789 ± 0.087
0.992GlnMet: 0.992 ± 0.035
1.438GlnAsn: 1.438 ± 0.039
2.051GlnPro: 2.051 ± 0.046
2.382GlnGln: 2.382 ± 0.058
2.743GlnArg: 2.743 ± 0.069
2.478GlnSer: 2.478 ± 0.054
2.158GlnThr: 2.158 ± 0.048
2.646GlnVal: 2.646 ± 0.05
0.581GlnTrp: 0.581 ± 0.028
1.078GlnTyr: 1.078 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
4.724ArgAla: 4.724 ± 0.076
0.502ArgCys: 0.502 ± 0.024
2.906ArgAsp: 2.906 ± 0.063
3.709ArgGlu: 3.709 ± 0.076
2.758ArgPhe: 2.758 ± 0.052
3.341ArgGly: 3.341 ± 0.064
1.679ArgHis: 1.679 ± 0.046
4.315ArgIle: 4.315 ± 0.074
2.682ArgLys: 2.682 ± 0.064
6.829ArgLeu: 6.829 ± 0.107
1.558ArgMet: 1.558 ± 0.042
2.319ArgAsn: 2.319 ± 0.054
2.28ArgPro: 2.28 ± 0.055
2.71ArgGln: 2.71 ± 0.06
3.328ArgArg: 3.328 ± 0.073
3.19ArgSer: 3.19 ± 0.062
2.716ArgThr: 2.716 ± 0.056
3.79ArgVal: 3.79 ± 0.057
0.91ArgTrp: 0.91 ± 0.033
2.251ArgTyr: 2.251 ± 0.056
0.0ArgXaa: 0.0 ± 0.0
Ser
5.267SerAla: 5.267 ± 0.089
0.599SerCys: 0.599 ± 0.028
3.153SerAsp: 3.153 ± 0.07
3.472SerGlu: 3.472 ± 0.055
2.436SerPhe: 2.436 ± 0.052
4.903SerGly: 4.903 ± 0.081
1.516SerHis: 1.516 ± 0.044
3.94SerIle: 3.94 ± 0.065
2.574SerLys: 2.574 ± 0.056
6.416SerLeu: 6.416 ± 0.094
1.545SerMet: 1.545 ± 0.04
2.115SerAsn: 2.115 ± 0.052
2.608SerPro: 2.608 ± 0.059
2.391SerGln: 2.391 ± 0.053
3.473SerArg: 3.473 ± 0.072
3.641SerSer: 3.641 ± 0.078
3.014SerThr: 3.014 ± 0.064
3.815SerVal: 3.815 ± 0.066
0.826SerTrp: 0.826 ± 0.029
1.632SerTyr: 1.632 ± 0.044
0.0SerXaa: 0.0 ± 0.0
Thr
4.794ThrAla: 4.794 ± 0.085
0.503ThrCys: 0.503 ± 0.025
2.774ThrAsp: 2.774 ± 0.064
3.058ThrGlu: 3.058 ± 0.062
2.068ThrPhe: 2.068 ± 0.059
4.196ThrGly: 4.196 ± 0.091
1.478ThrHis: 1.478 ± 0.038
3.535ThrIle: 3.535 ± 0.06
1.821ThrLys: 1.821 ± 0.05
6.58ThrLeu: 6.58 ± 0.093
1.093ThrMet: 1.093 ± 0.035
1.657ThrAsn: 1.657 ± 0.051
2.836ThrPro: 2.836 ± 0.059
2.247ThrGln: 2.247 ± 0.053
2.904ThrArg: 2.904 ± 0.061
3.003ThrSer: 3.003 ± 0.062
2.778ThrThr: 2.778 ± 0.065
3.651ThrVal: 3.651 ± 0.076
0.603ThrTrp: 0.603 ± 0.028
1.432ThrTyr: 1.432 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
6.073ValAla: 6.073 ± 0.103
0.678ValCys: 0.678 ± 0.032
3.595ValAsp: 3.595 ± 0.063
3.884ValGlu: 3.884 ± 0.064
2.673ValPhe: 2.673 ± 0.06
4.42ValGly: 4.42 ± 0.09
1.379ValHis: 1.379 ± 0.042
4.655ValIle: 4.655 ± 0.069
2.866ValLys: 2.866 ± 0.071
6.971ValLeu: 6.971 ± 0.096
1.823ValMet: 1.823 ± 0.049
2.501ValAsn: 2.501 ± 0.057
2.856ValPro: 2.856 ± 0.059
2.062ValGln: 2.062 ± 0.049
3.59ValArg: 3.59 ± 0.067
4.16ValSer: 4.16 ± 0.077
3.963ValThr: 3.963 ± 0.069
4.494ValVal: 4.494 ± 0.087
0.788ValTrp: 0.788 ± 0.027
1.716ValTyr: 1.716 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
0.901TrpAla: 0.901 ± 0.033
0.154TrpCys: 0.154 ± 0.014
0.588TrpAsp: 0.588 ± 0.029
0.639TrpGlu: 0.639 ± 0.027
0.605TrpPhe: 0.605 ± 0.027
0.789TrpGly: 0.789 ± 0.031
0.426TrpHis: 0.426 ± 0.023
0.828TrpIle: 0.828 ± 0.028
0.519TrpLys: 0.519 ± 0.023
1.946TrpLeu: 1.946 ± 0.053
0.31TrpMet: 0.31 ± 0.02
0.471TrpAsn: 0.471 ± 0.023
0.558TrpPro: 0.558 ± 0.026
0.904TrpGln: 0.904 ± 0.036
1.02TrpArg: 1.02 ± 0.037
0.766TrpSer: 0.766 ± 0.03
0.496TrpThr: 0.496 ± 0.027
0.952TrpVal: 0.952 ± 0.034
0.219TrpTrp: 0.219 ± 0.019
0.354TrpTyr: 0.354 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.572TyrAla: 2.572 ± 0.044
0.353TyrCys: 0.353 ± 0.021
1.509TyrAsp: 1.509 ± 0.043
1.456TyrGlu: 1.456 ± 0.039
1.308TyrPhe: 1.308 ± 0.04
2.106TyrGly: 2.106 ± 0.052
0.854TyrHis: 0.854 ± 0.034
1.494TyrIle: 1.494 ± 0.038
0.993TyrLys: 0.993 ± 0.035
3.418TyrLeu: 3.418 ± 0.07
0.534TyrMet: 0.534 ± 0.028
0.954TyrAsn: 0.954 ± 0.036
1.533TyrPro: 1.533 ± 0.045
1.513TyrGln: 1.513 ± 0.044
2.087TyrArg: 2.087 ± 0.051
1.648TyrSer: 1.648 ± 0.042
1.502TyrThr: 1.502 ± 0.037
1.715TyrVal: 1.715 ± 0.04
0.501TyrTrp: 0.501 ± 0.026
1.01TyrTyr: 1.01 ± 0.032
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2883 proteins (910118 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski