Wuhan Louse Fly Virus 9

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sigmavirus; Hippoboscid sigmavirus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KKB5|A0A0B5KKB5_9VIRU Matrix protein OS=Wuhan Louse Fly Virus 9 OX=1608123 GN=M PE=4 SV=1
MM1 pKa = 7.84EE2 pKa = 5.83SPNQDD7 pKa = 2.5SDD9 pKa = 4.33LEE11 pKa = 4.14EE12 pKa = 4.37MQDD15 pKa = 3.86KK16 pKa = 10.77LNKK19 pKa = 10.48LEE21 pKa = 4.7DD22 pKa = 3.81LDD24 pKa = 3.94VFKK27 pKa = 10.7EE28 pKa = 4.24IEE30 pKa = 4.15AQTQTNAADD39 pKa = 3.32WSIDD43 pKa = 3.22GKK45 pKa = 11.26KK46 pKa = 10.07LVPTDD51 pKa = 4.23FDD53 pKa = 4.79SDD55 pKa = 3.82SSDD58 pKa = 4.02YY59 pKa = 11.46EE60 pKa = 4.14DD61 pKa = 6.27DD62 pKa = 3.79EE63 pKa = 4.38ALKK66 pKa = 11.05LGTTILAKK74 pKa = 10.23PKK76 pKa = 10.44LIVPPPQDD84 pKa = 3.26EE85 pKa = 4.28RR86 pKa = 11.84QGPSGISFTPLQQTYY101 pKa = 9.1TDD103 pKa = 3.8YY104 pKa = 11.06QQSVKK109 pKa = 9.43QKK111 pKa = 10.23RR112 pKa = 11.84IRR114 pKa = 11.84TMTTGANYY122 pKa = 10.42NIVVNDD128 pKa = 3.74PRR130 pKa = 11.84FLVCPSDD137 pKa = 3.49LCNEE141 pKa = 4.23LGFLFYY147 pKa = 10.86AVATRR152 pKa = 11.84AIRR155 pKa = 11.84PEE157 pKa = 3.97QVCLYY162 pKa = 9.12FNSGGTQTMHH172 pKa = 6.34QGAGLNYY179 pKa = 10.09NIFISGLQQQLPPPQFPPPPPPPIPRR205 pKa = 11.84CSPILSNLPSLDD217 pKa = 3.4KK218 pKa = 10.87LIRR221 pKa = 11.84AADD224 pKa = 4.09PFPSHH229 pKa = 7.17PFVKK233 pKa = 10.31KK234 pKa = 9.72LQEE237 pKa = 4.55GIKK240 pKa = 10.3LKK242 pKa = 10.6HH243 pKa = 6.32LYY245 pKa = 9.98LSKK248 pKa = 10.03KK249 pKa = 8.37TNNDD253 pKa = 2.63LHH255 pKa = 7.49KK256 pKa = 10.39PAGFF260 pKa = 3.66

Molecular weight: 29.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KRS6|A0A0B5KRS6_9VIRU Glycoprotein OS=Wuhan Louse Fly Virus 9 OX=1608123 GN=G PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84MKK4 pKa = 10.25KK5 pKa = 10.08VKK7 pKa = 10.55NLVSPRR13 pKa = 11.84RR14 pKa = 11.84KK15 pKa = 10.04AKK17 pKa = 10.39SDD19 pKa = 3.38EE20 pKa = 4.39SNQASAVITPTAPSPSSTTPFSVPTIQPNIVPSIITSRR58 pKa = 11.84WNTVGSLRR66 pKa = 11.84ISCEE70 pKa = 3.7RR71 pKa = 11.84EE72 pKa = 3.41ITDD75 pKa = 3.23WVTMEE80 pKa = 4.45RR81 pKa = 11.84LLEE84 pKa = 4.0YY85 pKa = 10.41MLDD88 pKa = 3.76FYY90 pKa = 11.6DD91 pKa = 4.01GNEE94 pKa = 3.82VFKK97 pKa = 10.65PVILMLYY104 pKa = 8.8WILGVHH110 pKa = 6.78LKK112 pKa = 10.29PVEE115 pKa = 4.42GPSNRR120 pKa = 11.84WCWGIDD126 pKa = 3.28FGEE129 pKa = 4.59PIEE132 pKa = 4.43VKK134 pKa = 10.53HH135 pKa = 6.04KK136 pKa = 10.5FPLIGSQSFDD146 pKa = 3.11YY147 pKa = 10.66RR148 pKa = 11.84KK149 pKa = 10.59NITSNYY155 pKa = 9.75RR156 pKa = 11.84GIKK159 pKa = 9.97FNIEE163 pKa = 3.28FWIQFTGTQRR173 pKa = 11.84RR174 pKa = 11.84STPVKK179 pKa = 10.26DD180 pKa = 3.63LLGVGSLIFPDD191 pKa = 3.22VRR193 pKa = 11.84RR194 pKa = 11.84FKK196 pKa = 10.84EE197 pKa = 3.84ILDD200 pKa = 3.77FSNINCEE207 pKa = 3.82LDD209 pKa = 3.66SNKK212 pKa = 10.24NLVLTQQ218 pKa = 3.44

Molecular weight: 25.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3604

218

2145

720.8

82.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.635 ± 0.388

1.609 ± 0.145

5.161 ± 0.427

5.522 ± 0.54

4.412 ± 0.261

5.272 ± 0.28

2.58 ± 0.331

7.714 ± 0.274

6.243 ± 0.303

11.043 ± 0.713

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.831 ± 0.348

5.716 ± 0.26

5.3 ± 0.928

3.857 ± 0.427

4.55 ± 0.558

8.546 ± 0.294

5.966 ± 0.624

5.66 ± 0.659

1.693 ± 0.173

3.69 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski