Anaerocolumna cellulosilytica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Anaerocolumna

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4494 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S6R1X9|A0A6S6R1X9_9FIRM Thioredoxin OS=Anaerocolumna cellulosilytica OX=433286 GN=trx2 PE=3 SV=1
MM1 pKa = 7.72LIAINEE7 pKa = 4.13DD8 pKa = 3.27EE9 pKa = 4.54RR10 pKa = 11.84SIIHH14 pKa = 7.38DD15 pKa = 3.54MFDD18 pKa = 3.28NFIVLLLEE26 pKa = 4.66KK27 pKa = 10.82DD28 pKa = 3.38NALNMTGCNTYY39 pKa = 10.8KK40 pKa = 10.67EE41 pKa = 4.67FVDD44 pKa = 5.5DD45 pKa = 3.85MVEE48 pKa = 4.18YY49 pKa = 10.39ICC51 pKa = 6.17

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S6R6P8|A0A6S6R6P8_9FIRM FAD-dependent oxidoreductase OS=Anaerocolumna cellulosilytica OX=433286 GN=acsn021_27290 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.69VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.26GRR39 pKa = 11.84HH40 pKa = 5.5KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4494

0

4494

1532155

39

6555

340.9

38.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.407 ± 0.036

1.237 ± 0.012

5.302 ± 0.026

7.429 ± 0.041

4.284 ± 0.028

6.608 ± 0.034

1.681 ± 0.015

8.371 ± 0.033

7.56 ± 0.031

9.406 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.7 ± 0.018

5.254 ± 0.03

3.069 ± 0.021

3.134 ± 0.018

3.758 ± 0.026

6.215 ± 0.03

5.685 ± 0.037

6.427 ± 0.03

0.88 ± 0.014

4.591 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski