Mycobacterium phage WIVsmall

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4JHR4|R4JHR4_9CAUD Uncharacterized protein OS=Mycobacterium phage WIVsmall OX=1327036 GN=WIVsmall_69 PE=4 SV=1
MM1 pKa = 7.49AVTAKK6 pKa = 10.52YY7 pKa = 10.46YY8 pKa = 11.09SLFFKK13 pKa = 10.83SAFNKK18 pKa = 10.58EE19 pKa = 3.46IDD21 pKa = 4.33LDD23 pKa = 3.87SDD25 pKa = 3.94TIKK28 pKa = 11.21VMLCTSAYY36 pKa = 9.26TPNQDD41 pKa = 2.48THH43 pKa = 6.27QYY45 pKa = 9.51KK46 pKa = 10.67SSVTNEE52 pKa = 3.76VSGTGYY58 pKa = 8.12TAGGATISGISVSYY72 pKa = 10.2NASTNVLSFDD82 pKa = 3.7GADD85 pKa = 3.72VSWTGATLIGSNAPRR100 pKa = 11.84YY101 pKa = 9.24AVIYY105 pKa = 9.92DD106 pKa = 3.96ATPGSDD112 pKa = 3.01SARR115 pKa = 11.84PLIGYY120 pKa = 9.7VDD122 pKa = 4.89FGDD125 pKa = 5.37DD126 pKa = 3.3SYY128 pKa = 12.14APNGGTLTIAWNAAGIGSVTVSS150 pKa = 3.02

Molecular weight: 15.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4JN76|R4JN76_9CAUD Uncharacterized protein OS=Mycobacterium phage WIVsmall OX=1327036 GN=WIVsmall_59 PE=4 SV=1
MM1 pKa = 7.29NKK3 pKa = 9.07IHH5 pKa = 6.77IASHH9 pKa = 6.72GPAGWNATILFTAGTVYY26 pKa = 10.11TVADD30 pKa = 3.79DD31 pKa = 3.38QGRR34 pKa = 11.84KK35 pKa = 9.22HH36 pKa = 6.73LIDD39 pKa = 3.4TSRR42 pKa = 11.84VTVRR46 pKa = 11.84RR47 pKa = 11.84LSS49 pKa = 3.31

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

17522

34

1212

211.1

22.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.431 ± 0.574

1.307 ± 0.182

6.312 ± 0.309

5.336 ± 0.284

2.796 ± 0.22

8.835 ± 0.561

2.317 ± 0.243

4.4 ± 0.164

3.093 ± 0.222

7.505 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.157 ± 0.113

3.43 ± 0.202

6.118 ± 0.258

3.419 ± 0.258

6.997 ± 0.669

6.569 ± 0.35

6.552 ± 0.288

7.385 ± 0.334

1.832 ± 0.15

2.209 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski