endosymbiont of unidentified scaly snail isolate Monju

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; unclassified Gammaproteobacteria

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2228 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S6BY56|S6BY56_9GAMM Glutaredoxin OS=endosymbiont of unidentified scaly snail isolate Monju OX=1248727 GN=EBS_1184 PE=4 SV=1
MM1 pKa = 7.77LILDD5 pKa = 3.88FSGSVRR11 pKa = 11.84GQNLADD17 pKa = 3.51MQAAVISMLDD27 pKa = 3.16AYY29 pKa = 11.2NNAGYY34 pKa = 10.74AVVQLVTFSGNANIPADD51 pKa = 4.08GGWISVADD59 pKa = 4.04AKK61 pKa = 11.16AYY63 pKa = 10.1INGLTDD69 pKa = 3.76ADD71 pKa = 3.85MGGSTNYY78 pKa = 10.23DD79 pKa = 3.12AALAAAQSAFAEE91 pKa = 4.41AAGKK95 pKa = 10.22IPGAKK100 pKa = 9.77NIAYY104 pKa = 8.84FLSDD108 pKa = 3.88GSPTSGNGSIGIDD121 pKa = 3.6PAEE124 pKa = 3.79QAAWEE129 pKa = 4.1NFLTNNAIDD138 pKa = 3.53SHH140 pKa = 7.62AVGFGGASTTEE151 pKa = 4.29LEE153 pKa = 4.55PIAYY157 pKa = 9.98NGIDD161 pKa = 3.66GTEE164 pKa = 4.2CPALDD169 pKa = 3.87ATTAGANLTQVLLDD183 pKa = 3.87TVAQTVPGNLFGSLATGGFGADD205 pKa = 3.25GAGIVTSLTVGGVTYY220 pKa = 10.12AYY222 pKa = 10.46DD223 pKa = 3.86SNNDD227 pKa = 3.85TITGGSSTLNGHH239 pKa = 5.82QLTVTTSQGGILSVNMLTGEE259 pKa = 4.14YY260 pKa = 9.61TYY262 pKa = 11.19LADD265 pKa = 3.5PTFTISYY272 pKa = 9.65NEE274 pKa = 3.65IVAYY278 pKa = 10.07ALQDD282 pKa = 3.46ADD284 pKa = 4.07GDD286 pKa = 4.26ATSGTLTLNVARR298 pKa = 11.84DD299 pKa = 3.97VKK301 pKa = 10.49PVPTLLDD308 pKa = 3.33NTADD312 pKa = 3.57VYY314 pKa = 10.28EE315 pKa = 4.56AAMSTGTNPDD325 pKa = 2.89STAEE329 pKa = 4.1VATGNILSDD338 pKa = 3.57DD339 pKa = 4.48TIPAGLSLSNVSIAGGATVINGNTITVTTAEE370 pKa = 4.42GNTLVVDD377 pKa = 5.35KK378 pKa = 10.49ITGDD382 pKa = 3.41YY383 pKa = 10.33TYY385 pKa = 10.97TLNNPVKK392 pKa = 10.16HH393 pKa = 6.33LLFSATGNQVTLANDD408 pKa = 3.84TFTGGVLDD416 pKa = 4.6GWTGTNVSNKK426 pKa = 9.77NDD428 pKa = 3.24WLRR431 pKa = 11.84IDD433 pKa = 3.51GRR435 pKa = 11.84GDD437 pKa = 3.18VATKK441 pKa = 10.23TFDD444 pKa = 3.53FGQSYY449 pKa = 11.34ANQTVHH455 pKa = 5.08VTFDD459 pKa = 3.56FKK461 pKa = 11.77ANDD464 pKa = 3.03KK465 pKa = 10.06WDD467 pKa = 4.03ANTSDD472 pKa = 4.07SFRR475 pKa = 11.84MAVNGVEE482 pKa = 4.15ISNVPYY488 pKa = 10.59GKK490 pKa = 9.91NATDD494 pKa = 3.47TYY496 pKa = 11.19SFDD499 pKa = 3.49VTLDD503 pKa = 3.59ASGKK507 pKa = 10.63AYY509 pKa = 10.73FEE511 pKa = 4.54LTASTNSNKK520 pKa = 9.77EE521 pKa = 3.72DD522 pKa = 3.55AFVDD526 pKa = 3.43NFKK529 pKa = 9.36ITGPQLVPTPTDD541 pKa = 3.29VLVDD545 pKa = 3.64SFTYY549 pKa = 9.97TVTDD553 pKa = 4.21LGGTAYY559 pKa = 10.51NSKK562 pKa = 10.75LNVSIHH568 pKa = 7.3DD569 pKa = 4.32DD570 pKa = 3.6APIATTQNQQINVPQQDD587 pKa = 3.59TNLMVILDD595 pKa = 4.8LSGSMQGSRR604 pKa = 11.84LAAARR609 pKa = 11.84TAISNLIDD617 pKa = 3.42TYY619 pKa = 11.51NGYY622 pKa = 11.3GDD624 pKa = 3.8VAVKK628 pKa = 10.38LVTFSTLAQEE638 pKa = 4.37KK639 pKa = 8.44TSYY642 pKa = 10.15WMTASEE648 pKa = 4.52AKK650 pKa = 10.51AILATLSASGWTNYY664 pKa = 7.91DD665 pKa = 3.14TALAQAIQSWDD676 pKa = 3.4DD677 pKa = 3.4GSRR680 pKa = 11.84ITTPPSGGVIQNVAYY695 pKa = 9.65FISDD699 pKa = 3.8GQPNMNDD706 pKa = 3.02GDD708 pKa = 4.28TTVLANSNAGGTSGADD724 pKa = 3.13AGIQAAEE731 pKa = 4.14EE732 pKa = 4.47STT734 pKa = 3.51

Molecular weight: 75.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S6BA54|S6BA54_9GAMM Phosphopantetheine adenylyltransferase OS=endosymbiont of unidentified scaly snail isolate Monju OX=1248727 GN=coaD PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.08RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84AGRR28 pKa = 11.84KK29 pKa = 8.84IINARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.53GRR39 pKa = 11.84ARR41 pKa = 11.84LAPP44 pKa = 4.03

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2228

0

2228

708729

30

5867

318.1

35.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.01 ± 0.069

0.992 ± 0.022

5.869 ± 0.056

6.919 ± 0.063

3.362 ± 0.032

8.144 ± 0.059

2.549 ± 0.028

4.862 ± 0.041

2.869 ± 0.049

11.407 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.321 ± 0.024

2.417 ± 0.029

5.214 ± 0.037

3.729 ± 0.032

8.275 ± 0.059

4.505 ± 0.035

4.524 ± 0.038

7.293 ± 0.049

1.403 ± 0.026

2.336 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski