Staphylococcus phage phiBU01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Biseptimavirus; Staphylococcus virus BU01

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075LYF5|A0A075LYF5_9CAUD Uncharacterized protein OS=Staphylococcus phage phiBU01 OX=1519999 PE=4 SV=1
MM1 pKa = 7.41YY2 pKa = 9.78KK3 pKa = 9.44ATWFGIRR10 pKa = 11.84EE11 pKa = 4.1TEE13 pKa = 3.74ATNFILEE20 pKa = 4.32EE21 pKa = 3.96MEE23 pKa = 3.92MMNNRR28 pKa = 11.84EE29 pKa = 4.11QIEE32 pKa = 3.87QSIISASAYY41 pKa = 10.06NGNDD45 pKa = 3.29TEE47 pKa = 4.44GLLKK51 pKa = 10.44EE52 pKa = 4.35VEE54 pKa = 4.22DD55 pKa = 4.44VYY57 pKa = 11.66KK58 pKa = 10.66KK59 pKa = 10.77AQAFDD64 pKa = 4.77EE65 pKa = 4.31ILKK68 pKa = 10.03GLPNAMQDD76 pKa = 3.61ALKK79 pKa = 10.63EE80 pKa = 4.3DD81 pKa = 3.43IGLDD85 pKa = 3.48EE86 pKa = 5.32AVGIMTGQVVYY97 pKa = 10.18KK98 pKa = 10.68YY99 pKa = 10.98EE100 pKa = 3.83EE101 pKa = 4.11AQEE104 pKa = 4.1NEE106 pKa = 3.99

Molecular weight: 12.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075M056|A0A075M056_9CAUD Uncharacterized protein OS=Staphylococcus phage phiBU01 OX=1519999 PE=4 SV=1
MM1 pKa = 7.95DD2 pKa = 3.75KK3 pKa = 9.84MARR6 pKa = 11.84KK7 pKa = 9.61ARR9 pKa = 11.84IVTINDD15 pKa = 2.81KK16 pKa = 10.44PYY18 pKa = 10.06RR19 pKa = 11.84FSKK22 pKa = 11.02FEE24 pKa = 3.76MEE26 pKa = 5.0LIEE29 pKa = 4.34SHH31 pKa = 6.88GITAGMVSKK40 pKa = 10.43RR41 pKa = 11.84VKK43 pKa = 10.67DD44 pKa = 3.3GWEE47 pKa = 3.56LHH49 pKa = 5.97EE50 pKa = 6.15AMDD53 pKa = 4.24APEE56 pKa = 4.22GTRR59 pKa = 11.84LSEE62 pKa = 3.86YY63 pKa = 10.23RR64 pKa = 11.84EE65 pKa = 3.95KK66 pKa = 10.39KK67 pKa = 8.51TIEE70 pKa = 3.99RR71 pKa = 11.84LEE73 pKa = 3.92QARR76 pKa = 11.84LEE78 pKa = 4.26RR79 pKa = 11.84KK80 pKa = 9.75LEE82 pKa = 4.03RR83 pKa = 11.84KK84 pKa = 9.41RR85 pKa = 11.84KK86 pKa = 9.67RR87 pKa = 11.84EE88 pKa = 3.71AEE90 pKa = 3.97LRR92 pKa = 11.84RR93 pKa = 11.84KK94 pKa = 9.76KK95 pKa = 10.2PHH97 pKa = 6.56LFNVPQKK104 pKa = 10.45HH105 pKa = 4.8PRR107 pKa = 11.84GRR109 pKa = 11.84YY110 pKa = 8.83ACYY113 pKa = 10.52LMEE116 pKa = 5.36NDD118 pKa = 4.09IFVKK122 pKa = 10.41VKK124 pKa = 10.3KK125 pKa = 10.51

Molecular weight: 15.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

11393

74

1514

247.7

28.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.53 ± 0.43

0.535 ± 0.103

6.118 ± 0.365

7.759 ± 0.573

4.336 ± 0.28

5.793 ± 0.679

1.597 ± 0.129

7.987 ± 0.306

9.673 ± 0.306

7.97 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.156

6.785 ± 0.294

2.686 ± 0.166

3.651 ± 0.254

4.222 ± 0.3

6.039 ± 0.311

5.802 ± 0.237

5.653 ± 0.25

1.194 ± 0.181

4.345 ± 0.365
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski