Muir Springs virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Barhavirus; Muir barhavirus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3R160|A0A0D3R160_9RHAB GDP polyribonucleotidyltransferase OS=Muir Springs virus OX=932700 PE=4 SV=1
MM1 pKa = 7.49AVAILPVSRR10 pKa = 11.84NLPARR15 pKa = 11.84NRR17 pKa = 11.84TVAGSVTAPPVQYY30 pKa = 9.72PSEE33 pKa = 3.9WFRR36 pKa = 11.84ANEE39 pKa = 3.76NRR41 pKa = 11.84KK42 pKa = 9.64VSITIYY48 pKa = 10.01QQTTAGQAYY57 pKa = 10.16SRR59 pKa = 11.84IEE61 pKa = 3.64ALRR64 pKa = 11.84NNGQWDD70 pKa = 4.0DD71 pKa = 3.86VLISTFMRR79 pKa = 11.84GVIDD83 pKa = 5.48EE84 pKa = 4.15NAEE87 pKa = 4.04WFQSAPLTEE96 pKa = 4.35DD97 pKa = 2.66WSVNGAVIGQTGASITPLNLATWEE121 pKa = 4.27TIPRR125 pKa = 11.84PGNLGPIANQEE136 pKa = 4.19GEE138 pKa = 4.31LEE140 pKa = 4.18TRR142 pKa = 11.84RR143 pKa = 11.84SFFLALVTIYY153 pKa = 10.55RR154 pKa = 11.84QVLTRR159 pKa = 11.84DD160 pKa = 2.91INAEE164 pKa = 3.83YY165 pKa = 9.52GQEE168 pKa = 3.75VSRR171 pKa = 11.84RR172 pKa = 11.84IIDD175 pKa = 3.59NFKK178 pKa = 9.86EE179 pKa = 3.98QPLAMSQDD187 pKa = 4.5DD188 pKa = 3.95INNIQNFEE196 pKa = 4.3TKK198 pKa = 10.46DD199 pKa = 3.56KK200 pKa = 10.45LTANYY205 pKa = 10.21VKK207 pKa = 10.28VLCILDD213 pKa = 3.32MFFNKK218 pKa = 9.44FQNHH222 pKa = 6.72DD223 pKa = 3.27KK224 pKa = 9.88STIRR228 pKa = 11.84IATLPTRR235 pKa = 11.84YY236 pKa = 9.38RR237 pKa = 11.84GCAAFTSYY245 pKa = 11.65GEE247 pKa = 3.81LAIRR251 pKa = 11.84LGIEE255 pKa = 4.05PVQLPSLILTMAVARR270 pKa = 11.84DD271 pKa = 3.47FDD273 pKa = 4.49KK274 pKa = 11.26INIGGEE280 pKa = 4.09QAEE283 pKa = 4.42HH284 pKa = 6.8LDD286 pKa = 4.08GYY288 pKa = 10.88FPYY291 pKa = 10.62QLTLGLVKK299 pKa = 10.4KK300 pKa = 10.24SAYY303 pKa = 9.7SASNCPALYY312 pKa = 10.41LWMHH316 pKa = 7.01TIGTMLYY323 pKa = 7.42QQRR326 pKa = 11.84SYY328 pKa = 10.8RR329 pKa = 11.84AQVPKK334 pKa = 10.69NVPDD338 pKa = 3.36QMGTINSAIAVAIQYY353 pKa = 9.04VAGGDD358 pKa = 3.59FSMQFVADD366 pKa = 3.81ARR368 pKa = 11.84VQGAMRR374 pKa = 11.84EE375 pKa = 4.24MQDD378 pKa = 3.12AEE380 pKa = 4.55AEE382 pKa = 4.34LTHH385 pKa = 6.73LRR387 pKa = 11.84QLTARR392 pKa = 11.84AARR395 pKa = 11.84QAAEE399 pKa = 4.2GNVNDD404 pKa = 4.04EE405 pKa = 4.72GGDD408 pKa = 3.52EE409 pKa = 4.54GEE411 pKa = 4.2SSEE414 pKa = 5.57DD415 pKa = 3.39EE416 pKa = 4.37EE417 pKa = 5.89EE418 pKa = 4.49EE419 pKa = 5.63DD420 pKa = 5.13DD421 pKa = 5.43HH422 pKa = 8.95QLDD425 pKa = 4.39DD426 pKa = 4.6QDD428 pKa = 4.68DD429 pKa = 4.13PEE431 pKa = 4.6IAARR435 pKa = 11.84LEE437 pKa = 4.48RR438 pKa = 11.84IGVLQEE444 pKa = 3.41NLRR447 pKa = 11.84RR448 pKa = 11.84CTATVEE454 pKa = 4.22QFTSVVEE461 pKa = 4.21KK462 pKa = 10.78SAMRR466 pKa = 11.84ALAYY470 pKa = 10.14LQEE473 pKa = 4.43NGGIAEE479 pKa = 4.14KK480 pKa = 10.57DD481 pKa = 3.25RR482 pKa = 11.84RR483 pKa = 11.84EE484 pKa = 3.67LGIRR488 pKa = 11.84FRR490 pKa = 11.84RR491 pKa = 11.84FADD494 pKa = 3.61EE495 pKa = 3.82ADD497 pKa = 3.45GRR499 pKa = 11.84VGKK502 pKa = 10.29LLANLFPAHH511 pKa = 6.77RR512 pKa = 4.62

Molecular weight: 57.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3R124|A0A0D3R124_9RHAB Nucleocapsid protein OS=Muir Springs virus OX=932700 PE=4 SV=1
MM1 pKa = 7.6SYY3 pKa = 8.9STGLIRR9 pKa = 11.84SEE11 pKa = 4.45VSSEE15 pKa = 3.57MSKK18 pKa = 10.81AFKK21 pKa = 10.43DD22 pKa = 3.53AGEE25 pKa = 4.12HH26 pKa = 5.97QIALNKK32 pKa = 10.38EE33 pKa = 4.03YY34 pKa = 11.03DD35 pKa = 3.66NMSSISTNMNALKK48 pKa = 11.07NMFNPDD54 pKa = 3.94DD55 pKa = 5.07DD56 pKa = 5.22SNSDD60 pKa = 3.3GTSPSSRR67 pKa = 11.84NSILLASDD75 pKa = 3.62MFLGNDD81 pKa = 4.41DD82 pKa = 4.25YY83 pKa = 12.11GSDD86 pKa = 3.78DD87 pKa = 3.26SHH89 pKa = 7.67NFLSSPSPDD98 pKa = 2.94RR99 pKa = 11.84DD100 pKa = 3.55SSEE103 pKa = 4.18EE104 pKa = 3.78GSKK107 pKa = 9.77IQEE110 pKa = 3.89FRR112 pKa = 11.84FEE114 pKa = 4.03IPKK117 pKa = 10.65SKK119 pKa = 9.47TYY121 pKa = 10.63KK122 pKa = 9.75DD123 pKa = 2.83RR124 pKa = 11.84SYY126 pKa = 11.04RR127 pKa = 11.84RR128 pKa = 11.84GVIDD132 pKa = 4.82VLDD135 pKa = 3.95FLQRR139 pKa = 11.84HH140 pKa = 5.36HH141 pKa = 7.0FIEE144 pKa = 4.48EE145 pKa = 3.89FLFEE149 pKa = 4.83GDD151 pKa = 3.42HH152 pKa = 6.43EE153 pKa = 4.89EE154 pKa = 4.99IIVITPTKK162 pKa = 10.75GMIPFKK168 pKa = 10.31RR169 pKa = 11.84SKK171 pKa = 10.98SEE173 pKa = 3.92DD174 pKa = 3.33SQIPMANEE182 pKa = 3.26KK183 pKa = 10.62DD184 pKa = 3.41IEE186 pKa = 4.21KK187 pKa = 10.98EE188 pKa = 4.02EE189 pKa = 3.88IEE191 pKa = 4.7KK192 pKa = 10.25PVKK195 pKa = 10.26SDD197 pKa = 2.99ARR199 pKa = 11.84NTTSEE204 pKa = 4.2HH205 pKa = 5.3STSIEE210 pKa = 3.96DD211 pKa = 3.44PKK213 pKa = 11.45NRR215 pKa = 11.84LKK217 pKa = 10.8EE218 pKa = 3.99ALRR221 pKa = 11.84KK222 pKa = 9.57NIKK225 pKa = 10.16DD226 pKa = 3.6DD227 pKa = 3.84LRR229 pKa = 11.84SKK231 pKa = 10.28ISKK234 pKa = 9.59NVPKK238 pKa = 10.43KK239 pKa = 10.79QEE241 pKa = 3.82TADD244 pKa = 3.79DD245 pKa = 3.88NKK247 pKa = 11.08EE248 pKa = 3.95GVRR251 pKa = 11.84PTNQKK256 pKa = 10.4EE257 pKa = 4.25MIPSQDD263 pKa = 3.1MTKK266 pKa = 8.95STKK269 pKa = 10.05KK270 pKa = 10.17QEE272 pKa = 4.26LSAKK276 pKa = 10.23PPIKK280 pKa = 10.25DD281 pKa = 3.42GKK283 pKa = 10.54KK284 pKa = 10.04KK285 pKa = 9.9EE286 pKa = 4.16DD287 pKa = 3.4KK288 pKa = 10.52KK289 pKa = 11.39AEE291 pKa = 4.08DD292 pKa = 3.26THH294 pKa = 9.47KK295 pKa = 11.0SFNYY299 pKa = 10.5ANTFGSKK306 pKa = 7.62VTVKK310 pKa = 9.82TSKK313 pKa = 10.13YY314 pKa = 10.26CKK316 pKa = 8.76NCKK319 pKa = 9.7AATRR323 pKa = 11.84KK324 pKa = 9.63NATIYY329 pKa = 10.93LNHH332 pKa = 7.47LYY334 pKa = 9.8TNHH337 pKa = 5.37SHH339 pKa = 7.09EE340 pKa = 4.09IALIKK345 pKa = 10.6SLAYY349 pKa = 9.56PLL351 pKa = 4.17

Molecular weight: 39.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3850

220

2175

770.0

88.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.571 ± 1.113

1.403 ± 0.294

5.792 ± 0.471

7.74 ± 0.396

4.338 ± 0.318

5.403 ± 0.43

2.208 ± 0.221

7.558 ± 0.381

7.325 ± 1.074

9.325 ± 1.123

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.468 ± 0.111

5.558 ± 0.209

3.948 ± 0.288

3.299 ± 0.64

5.377 ± 0.414

7.325 ± 0.922

5.844 ± 0.139

5.169 ± 0.553

1.506 ± 0.323

3.844 ± 0.288
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski