Pseudolysobacter antarcticus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Pseudolysobacter

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3894 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411HLQ0|A0A411HLQ0_9GAMM Ribosome maturation factor RimP OS=Pseudolysobacter antarcticus OX=2511995 GN=rimP PE=3 SV=1
MM1 pKa = 7.44NIIHH5 pKa = 7.03FLRR8 pKa = 11.84GVPRR12 pKa = 11.84RR13 pKa = 11.84FAAFVFGICATLPAFADD30 pKa = 3.68SSTLTISAASVHH42 pKa = 6.28VDD44 pKa = 3.31NAPTTLYY51 pKa = 10.77FPITRR56 pKa = 11.84SGDD59 pKa = 3.01ISYY62 pKa = 8.12DD63 pKa = 3.28TVLNYY68 pKa = 8.76HH69 pKa = 5.82TVDD72 pKa = 3.58GSAMAGIDD80 pKa = 3.43YY81 pKa = 7.64TANTGSVSLLSGSSSASIPVTITPSAPTSASNSFKK116 pKa = 10.44MLLDD120 pKa = 3.34STINLIAPGFAAHH133 pKa = 6.53VEE135 pKa = 4.27TSLGFDD141 pKa = 3.74VYY143 pKa = 11.41ALSTADD149 pKa = 4.09LNGDD153 pKa = 4.38GKK155 pKa = 10.04PDD157 pKa = 3.77LAIVSYY163 pKa = 11.07GSDD166 pKa = 2.75KK167 pKa = 11.23VSFLLNTTPAGAATPSFGAVQSINAGCAPQSIITADD203 pKa = 3.18INGDD207 pKa = 3.63GKK209 pKa = 10.12PDD211 pKa = 3.41VVFACPFDD219 pKa = 3.96NKK221 pKa = 9.24VTLLMNTTATGASTTSFATQVFTVAGPSSLKK252 pKa = 10.24ATDD255 pKa = 4.19LNGDD259 pKa = 3.66NKK261 pKa = 10.85LDD263 pKa = 5.51LIVTDD268 pKa = 3.65TNNYY272 pKa = 8.19QVVVMLNSTTVDD284 pKa = 3.04ASSASFFGTQAFATGMVPDD303 pKa = 6.28AITSTDD309 pKa = 2.98INGDD313 pKa = 3.66GKK315 pKa = 10.17PDD317 pKa = 3.42LVVSNRR323 pKa = 11.84LSNSLSVLLNTTANGAATASFAAQQVFATGTNPVWVSAIDD363 pKa = 3.4INGDD367 pKa = 3.65GKK369 pKa = 10.18PDD371 pKa = 3.56LAVANYY377 pKa = 9.69GSSTFSILLNTTTTGAAIPSFAPQQIFPTGSHH409 pKa = 6.05PTSIVSADD417 pKa = 3.45VNSDD421 pKa = 2.97GKK423 pKa = 9.47TDD425 pKa = 4.13LLVTNSFDD433 pKa = 3.31NTLSVFSNYY442 pKa = 7.37TAIGSSTAGFTINLYY457 pKa = 9.16ATGFSPQSGFAIDD470 pKa = 4.53LNADD474 pKa = 3.08GRR476 pKa = 11.84LDD478 pKa = 4.09LLTVNASGGDD488 pKa = 3.45VSVLMNTTVPPALSFASMQNLGTGGYY514 pKa = 8.09TNGVTSADD522 pKa = 3.27INGDD526 pKa = 3.35GKK528 pKa = 11.29LDD530 pKa = 4.64LIEE533 pKa = 4.48VDD535 pKa = 4.05RR536 pKa = 11.84SDD538 pKa = 3.31NTVSVFINTTPAGSATTSYY557 pKa = 11.22APRR560 pKa = 11.84RR561 pKa = 11.84TFATSSLPWAVAVADD576 pKa = 4.5LNGDD580 pKa = 4.29GKK582 pKa = 10.18PDD584 pKa = 3.04IAVANFFGSSVSVLTNQTATGATTASFSAQATFNTGSSPISIAITDD630 pKa = 3.52INGDD634 pKa = 3.56GKK636 pKa = 11.29LDD638 pKa = 3.6ILVADD643 pKa = 5.01AGANDD648 pKa = 3.61VAVLRR653 pKa = 11.84NTTTTGAATPSFAAYY668 pKa = 10.4LSLPAGGAPSAVASADD684 pKa = 3.55LNGDD688 pKa = 3.59GKK690 pKa = 11.36SDD692 pKa = 3.01IVVTNKK698 pKa = 10.44DD699 pKa = 3.64DD700 pKa = 3.77GTVSVFQNTTTVATTPTFAAQQILSVGSQPYY731 pKa = 9.75SVAIADD737 pKa = 3.84FNGDD741 pKa = 3.21GKK743 pKa = 11.0RR744 pKa = 11.84DD745 pKa = 3.65VVVANYY751 pKa = 10.23ASSTVSILLNTTTAPGATVSLAAQQTFDD779 pKa = 3.71GGNPYY784 pKa = 10.92AVTTADD790 pKa = 3.24INGDD794 pKa = 3.68GKK796 pKa = 10.1PDD798 pKa = 3.66VIVAVPGNSTAAVLLNTSASGTLNFASPMYY828 pKa = 10.33LSIGGSPTAIATTDD842 pKa = 3.33ANSDD846 pKa = 3.4GRR848 pKa = 11.84VDD850 pKa = 3.28IVSTNGGSNSLSILLNSQILTNLAGSPATGTIVHH884 pKa = 6.79DD885 pKa = 5.21LIVTPSAGEE894 pKa = 3.87NGSISQSSPQTVAYY908 pKa = 7.23GTSTSFTVTPLPNYY922 pKa = 8.64VATVVGTCGGTLLGTTYY939 pKa = 7.88TTAAITANCTVIASFAAVTHH959 pKa = 6.07IVMPSADD966 pKa = 3.4AHH968 pKa = 6.26GSISPNMAQNIDD980 pKa = 3.75HH981 pKa = 7.17GSTTMFTIMPDD992 pKa = 3.05TGYY995 pKa = 10.3SASVSGTCGGTLVDD1009 pKa = 4.24ATYY1012 pKa = 7.29TTNAITTDD1020 pKa = 3.69CSVVASFTLKK1030 pKa = 10.53SYY1032 pKa = 11.02LVTPSVGDD1040 pKa = 3.63YY1041 pKa = 11.4GSISPDD1047 pKa = 2.98TPQNIDD1053 pKa = 3.29YY1054 pKa = 10.21GATTSFMVTPNAGFIVIVGGTCGGTLVDD1082 pKa = 3.96STYY1085 pKa = 8.1TTNTITANCTVAVTFSDD1102 pKa = 4.89RR1103 pKa = 11.84IFADD1107 pKa = 4.05GFEE1110 pKa = 4.28QPP1112 pKa = 4.2

Molecular weight: 112.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411HPS7|A0A411HPS7_9GAMM Patatin-like phospholipase family protein OS=Pseudolysobacter antarcticus OX=2511995 GN=ELE36_20075 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.08RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 6.05GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 9.02TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.61GRR39 pKa = 11.84KK40 pKa = 9.07RR41 pKa = 11.84LTPP44 pKa = 3.95

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3894

0

3894

1372272

30

4690

352.4

38.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.178 ± 0.051

0.972 ± 0.015

5.533 ± 0.028

4.716 ± 0.051

3.738 ± 0.024

7.961 ± 0.052

2.365 ± 0.025

5.282 ± 0.027

3.19 ± 0.036

10.676 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.05 ± 0.022

3.459 ± 0.04

4.876 ± 0.024

3.788 ± 0.026

6.305 ± 0.056

6.316 ± 0.045

5.748 ± 0.076

6.967 ± 0.031

1.365 ± 0.018

2.515 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski