Leptospira phage vB_LalZ_80412-LE1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5WIH2|S5WIH2_9CAUD Uncharacterized protein OS=Leptospira phage vB_LalZ_80412-LE1 OX=1334242 GN=LEP1GSC193_0783 PE=4 SV=1
MM1 pKa = 7.9KK2 pKa = 10.29LRR4 pKa = 11.84FVGIVFFGLSILFILNFSLLNGISNIFNAVASAIAGGIPQKK45 pKa = 10.97LPIIQAGPDD54 pKa = 3.68GSASTQVEE62 pKa = 4.05IEE64 pKa = 4.09IPPGKK69 pKa = 10.21VIPEE73 pKa = 4.43LSLSYY78 pKa = 10.88NSNGGNGVVGYY89 pKa = 9.41GWSLNGVPTISRR101 pKa = 11.84NPSTGINYY109 pKa = 9.8NGSDD113 pKa = 3.7SYY115 pKa = 11.59ISGLAGEE122 pKa = 5.08LLDD125 pKa = 4.73ISGNKK130 pKa = 7.71TKK132 pKa = 10.71YY133 pKa = 9.68HH134 pKa = 5.88SKK136 pKa = 10.12KK137 pKa = 10.16EE138 pKa = 3.94SWVLYY143 pKa = 9.44EE144 pKa = 4.12PQGTCGDD151 pKa = 4.71GPCTWIATDD160 pKa = 3.61KK161 pKa = 10.89DD162 pKa = 3.35GKK164 pKa = 10.69RR165 pKa = 11.84FIFGGSIDD173 pKa = 3.6SRR175 pKa = 11.84IPALGRR181 pKa = 11.84TAGSIRR187 pKa = 11.84EE188 pKa = 4.0WALSRR193 pKa = 11.84EE194 pKa = 4.32EE195 pKa = 4.77DD196 pKa = 3.7SHH198 pKa = 8.19GNGYY202 pKa = 10.48DD203 pKa = 3.11ITYY206 pKa = 9.63TPIDD210 pKa = 3.94VTNGDD215 pKa = 4.35YY216 pKa = 11.13YY217 pKa = 10.67PNTITYY223 pKa = 9.68NDD225 pKa = 3.21RR226 pKa = 11.84TIRR229 pKa = 11.84FNYY232 pKa = 9.07EE233 pKa = 3.22NRR235 pKa = 11.84NDD237 pKa = 4.19KK238 pKa = 10.51IPNYY242 pKa = 10.7SLGTLIRR249 pKa = 11.84TQRR252 pKa = 11.84RR253 pKa = 11.84LNTIDD258 pKa = 3.48VLVGGTTFRR267 pKa = 11.84TYY269 pKa = 11.37DD270 pKa = 3.47LDD272 pKa = 3.95YY273 pKa = 8.67TTGPVTGRR281 pKa = 11.84SVLQKK286 pKa = 10.17IKK288 pKa = 10.59RR289 pKa = 11.84SGSNTFGSEE298 pKa = 3.43NFADD302 pKa = 5.02LNFTYY307 pKa = 10.12TNHH310 pKa = 6.55SGNFSPGNIDD320 pKa = 3.71YY321 pKa = 7.53QTLSNTISMAVFMPNIALDD340 pKa = 3.41ILNVYY345 pKa = 9.93FNGGLPYY352 pKa = 10.31HH353 pKa = 6.91PSALDD358 pKa = 3.7SNVDD362 pKa = 2.99ASLQYY367 pKa = 9.96IVKK370 pKa = 10.1VPVPDD375 pKa = 4.69RR376 pKa = 11.84NACNLGFASCLCAALPLCWGGNAGFFNYY404 pKa = 10.29LAGNCLSFLSWGGPGACDD422 pKa = 3.46NGVDD426 pKa = 3.95SALTAWLPMDD436 pKa = 4.78LNGDD440 pKa = 4.43GILDD444 pKa = 4.25FATLNGNEE452 pKa = 4.42TNGSIHH458 pKa = 6.7LVGHH462 pKa = 5.9IQRR465 pKa = 11.84IGQGPVTFNGPNIPIHH481 pKa = 6.44YY482 pKa = 7.24NTYY485 pKa = 8.81YY486 pKa = 10.7QPVDD490 pKa = 3.84LNGDD494 pKa = 3.74GKK496 pKa = 9.39TDD498 pKa = 3.86FAYY501 pKa = 10.26EE502 pKa = 4.65DD503 pKa = 4.67GGQLWAVYY511 pKa = 8.77STGGNFSSPVAFGNVSLAGSNRR533 pKa = 11.84DD534 pKa = 3.13MKK536 pKa = 11.09VFSPYY541 pKa = 10.17EE542 pKa = 3.65YY543 pKa = 10.09RR544 pKa = 11.84FQYY547 pKa = 10.57SPSNTTPLASNRR559 pKa = 11.84ATSDD563 pKa = 3.14FFADD567 pKa = 3.7MNGDD571 pKa = 3.89DD572 pKa = 4.41LTDD575 pKa = 4.17FVHH578 pKa = 7.09SNGGSFSIYY587 pKa = 9.98INRR590 pKa = 11.84EE591 pKa = 3.86TYY593 pKa = 9.82FDD595 pKa = 3.72NPIVIGGGNDD605 pKa = 3.55FYY607 pKa = 10.78INSMIDD613 pKa = 2.97MTGDD617 pKa = 3.26GKK619 pKa = 11.11SDD621 pKa = 3.68YY622 pKa = 10.27VQLIATYY629 pKa = 10.67DD630 pKa = 3.57NSTLTTLQAQKK641 pKa = 10.94AALDD645 pKa = 3.64VLMAQYY651 pKa = 10.47QVDD654 pKa = 3.82HH655 pKa = 6.74ARR657 pKa = 11.84VKK659 pKa = 10.68AVADD663 pKa = 4.7LYY665 pKa = 10.64PLALGVTVDD674 pKa = 3.69ANEE677 pKa = 3.86FQYY680 pKa = 10.75MIDD683 pKa = 3.63YY684 pKa = 10.85LNTNGYY690 pKa = 10.35DD691 pKa = 3.5PLADD695 pKa = 3.76FWEE698 pKa = 5.04SNGSGYY704 pKa = 10.94AYY706 pKa = 10.45NSSEE710 pKa = 3.93IGNLQTSLEE719 pKa = 4.43NIVSAKK725 pKa = 9.46MNFVGQQSQAINIQIASIYY744 pKa = 9.98AQGTLGQATYY754 pKa = 10.74ALQVRR759 pKa = 11.84TFNTSNGTSQVQTFPISNSIDD780 pKa = 3.37ADD782 pKa = 3.81RR783 pKa = 11.84STLADD788 pKa = 3.54VNGDD792 pKa = 3.42GALDD796 pKa = 3.72FVSFIGTQVSVSIFTGNGFAPPVVTGLNAGNGKK829 pKa = 9.05NLVQFNFGEE838 pKa = 4.4VNGDD842 pKa = 3.68GLSDD846 pKa = 3.61LVLFNRR852 pKa = 11.84EE853 pKa = 3.49SHH855 pKa = 7.01IIEE858 pKa = 4.71TYY860 pKa = 10.57LSRR863 pKa = 11.84GDD865 pKa = 3.69GGFTLSGGYY874 pKa = 9.94SFGGFSTQEE883 pKa = 3.87YY884 pKa = 8.53TDD886 pKa = 3.44SNGIEE891 pKa = 4.19RR892 pKa = 11.84SDD894 pKa = 4.02LYY896 pKa = 10.99QISLQDD902 pKa = 3.33INLDD906 pKa = 4.24GISS909 pKa = 3.31

Molecular weight: 98.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5VXX3|S5VXX3_9CAUD Uncharacterized protein OS=Leptospira phage vB_LalZ_80412-LE1 OX=1334242 GN=LEP1GSC193_0717 PE=4 SV=1
MM1 pKa = 7.63CSSKK5 pKa = 11.32SNAFNGGSSTSSPVLLLTQLIKK27 pKa = 10.5FAITLKK33 pKa = 10.36SLCFSSLLGPYY44 pKa = 8.87PQARR48 pKa = 11.84LYY50 pKa = 10.88SSSSKK55 pKa = 10.31KK56 pKa = 10.32VCLEE60 pKa = 3.67SPIFIFYY67 pKa = 10.3RR68 pKa = 11.84FSIRR72 pKa = 11.84SIGASSFSRR81 pKa = 11.84ILGCIVRR88 pKa = 11.84RR89 pKa = 11.84YY90 pKa = 9.65RR91 pKa = 11.84IVYY94 pKa = 9.04GILVRR99 pKa = 11.84ILSLTFVQNLGLMFLFKK116 pKa = 10.88LIGSTSQIFHH126 pKa = 7.28PKK128 pKa = 9.99AKK130 pKa = 9.88PLVSLFEE137 pKa = 3.94VMLIYY142 pKa = 10.56FLLPKK147 pKa = 10.02PVANSQFFPITQSLRR162 pKa = 11.84ILHH165 pKa = 6.06NSEE168 pKa = 3.91SRR170 pKa = 11.84NPHH173 pKa = 6.96PDD175 pKa = 2.89LQRR178 pKa = 11.84YY179 pKa = 8.0LLQILPFGLSTILFRR194 pKa = 11.84NSRR197 pKa = 11.84NN198 pKa = 3.32

Molecular weight: 22.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

24630

37

1116

256.6

28.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.876 ± 0.232

0.824 ± 0.072

4.815 ± 0.169

6.764 ± 0.414

5.493 ± 0.246

7.077 ± 0.393

1.429 ± 0.082

7.089 ± 0.237

7.84 ± 0.536

9.704 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.433 ± 0.089

6.074 ± 0.193

4.056 ± 0.166

3.228 ± 0.114

4.499 ± 0.169

8.368 ± 0.283

6.098 ± 0.291

5.416 ± 0.165

1.137 ± 0.07

3.78 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski