Quasibacillus thermotolerans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Quasibacillus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4016 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F5I703|A0A0F5I703_9BACI Uncharacterized protein OS=Quasibacillus thermotolerans OX=1221996 GN=QY95_01020 PE=4 SV=1
MM1 pKa = 7.64AKK3 pKa = 8.9YY4 pKa = 9.07TIVDD8 pKa = 3.48KK9 pKa = 9.83DD10 pKa = 3.47TCIACGACGATAPDD24 pKa = 3.09IFDD27 pKa = 3.74YY28 pKa = 11.35DD29 pKa = 4.13EE30 pKa = 5.02EE31 pKa = 4.48GLSYY35 pKa = 11.06VILDD39 pKa = 4.31DD40 pKa = 4.4NEE42 pKa = 4.09GTAKK46 pKa = 10.38VPEE49 pKa = 4.55DD50 pKa = 4.44LYY52 pKa = 11.65DD53 pKa = 4.32DD54 pKa = 5.99LEE56 pKa = 4.65DD57 pKa = 3.88ACEE60 pKa = 4.29GCPTEE65 pKa = 4.32SIKK68 pKa = 10.79VEE70 pKa = 4.01EE71 pKa = 4.68EE72 pKa = 3.97PFHH75 pKa = 7.45SKK77 pKa = 10.17VNAA80 pKa = 3.87

Molecular weight: 8.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F5I245|A0A0F5I245_9BACI Uncharacterized protein OS=Quasibacillus thermotolerans OX=1221996 GN=QY95_02111 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 10.24VHH16 pKa = 6.26GFRR19 pKa = 11.84QRR21 pKa = 11.84MSSKK25 pKa = 9.55NGRR28 pKa = 11.84RR29 pKa = 11.84ILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.95GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4016

0

4016

1082374

37

1515

269.5

30.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.014 ± 0.045

0.73 ± 0.012

4.817 ± 0.031

8.139 ± 0.051

4.402 ± 0.032

7.03 ± 0.04

2.159 ± 0.02

7.014 ± 0.037

6.492 ± 0.041

9.791 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.829 ± 0.02

3.677 ± 0.026

3.804 ± 0.023

3.85 ± 0.028

4.595 ± 0.034

5.907 ± 0.03

5.21 ± 0.025

7.148 ± 0.031

1.045 ± 0.015

3.345 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski