Mycolicibacterium doricum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia;

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3539 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X1T4L6|A0A1X1T4L6_9MYCO Uncharacterized protein OS=Mycolicibacterium doricum OX=126673 GN=AWC01_12960 PE=4 SV=1
MM1 pKa = 7.65IEE3 pKa = 3.97RR4 pKa = 11.84AITASDD10 pKa = 3.2NAAADD15 pKa = 4.27EE16 pKa = 4.45LWASLGDD23 pKa = 3.74PAAAAAAVHH32 pKa = 5.99QVLTDD37 pKa = 3.78GANPDD42 pKa = 3.53VYY44 pKa = 11.14VQAEE48 pKa = 4.49QIRR51 pKa = 11.84PPYY54 pKa = 9.88SPYY57 pKa = 10.94GQTIWPQADD66 pKa = 3.23AARR69 pKa = 11.84FAWTLPCIPDD79 pKa = 4.11ADD81 pKa = 3.77PVLAQMRR88 pKa = 11.84NIASGQQWGLAALDD102 pKa = 3.58NAATKK107 pKa = 10.5GGWGPDD113 pKa = 3.16PDD115 pKa = 4.19GNYY118 pKa = 10.15LARR121 pKa = 11.84QIGVYY126 pKa = 7.14QTEE129 pKa = 4.25TGALGLAIATEE140 pKa = 4.3PDD142 pKa = 3.56DD143 pKa = 4.0GTFATATSILNNPANWITQNTAEE166 pKa = 4.77LPGAGCTAVV175 pKa = 3.17

Molecular weight: 18.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X1TK85|A0A1X1TK85_9MYCO Uncharacterized protein OS=Mycolicibacterium doricum OX=126673 GN=AWC01_02260 PE=4 SV=1
MM1 pKa = 7.41SRR3 pKa = 11.84FRR5 pKa = 11.84LRR7 pKa = 11.84RR8 pKa = 11.84LRR10 pKa = 11.84PVLRR14 pKa = 11.84GAPALLPGRR23 pKa = 11.84RR24 pKa = 11.84LSLPVWSSPLPVRR37 pKa = 11.84LRR39 pKa = 11.84LHH41 pKa = 7.14RR42 pKa = 11.84LPLSLSRR49 pKa = 11.84RR50 pKa = 11.84QVSRR54 pKa = 11.84IRR56 pKa = 11.84HH57 pKa = 4.73RR58 pKa = 11.84RR59 pKa = 11.84LRR61 pKa = 11.84VLRR64 pKa = 11.84RR65 pKa = 11.84VVRR68 pKa = 11.84VGQQ71 pKa = 3.39

Molecular weight: 8.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3539

0

3539

1113055

37

3076

314.5

33.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.019 ± 0.062

0.792 ± 0.012

6.389 ± 0.037

5.388 ± 0.039

2.905 ± 0.022

8.892 ± 0.04

2.2 ± 0.022

4.035 ± 0.026

2.158 ± 0.029

9.857 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.022 ± 0.018

2.119 ± 0.02

5.804 ± 0.038

2.997 ± 0.022

7.559 ± 0.04

5.293 ± 0.027

6.024 ± 0.025

9.009 ± 0.041

1.461 ± 0.017

2.077 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski