Alkalibacterium gilvum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Carnobacteriaceae; Alkalibacterium

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H6UL70|A0A1H6UL70_9LACT Thiol peroxidase atypical 2-Cys peroxiredoxin OS=Alkalibacterium gilvum OX=1130080 GN=SAMN04488113_13417 PE=4 SV=1
MM1 pKa = 7.54TIIIGITLFTGFKK14 pKa = 9.5TYY16 pKa = 10.63QIAQAFGSPLNRR28 pKa = 11.84NIEE31 pKa = 3.69EE32 pKa = 3.87WLYY35 pKa = 10.78IKK37 pKa = 9.85TVDD40 pKa = 3.55YY41 pKa = 11.61EE42 pKa = 4.22NFNVFDD48 pKa = 3.74QSVEE52 pKa = 4.06EE53 pKa = 4.61LFTMIDD59 pKa = 3.78EE60 pKa = 4.71EE61 pKa = 4.81VDD63 pKa = 4.03LPDD66 pKa = 4.32PLYY69 pKa = 10.4IYY71 pKa = 11.08NEE73 pKa = 3.84FDD75 pKa = 4.19LEE77 pKa = 4.12FDD79 pKa = 3.81RR80 pKa = 11.84SGQLTSFYY88 pKa = 11.07AAVTGEE94 pKa = 3.95NEE96 pKa = 3.95EE97 pKa = 5.24GVFEE101 pKa = 4.32WFLISDD107 pKa = 3.78SDD109 pKa = 4.04EE110 pKa = 4.5PNQLTINSGEE120 pKa = 4.04NPDD123 pKa = 5.24RR124 pKa = 11.84EE125 pKa = 4.31VLSNNMLLSPLFDD138 pKa = 3.85TLDD141 pKa = 3.51QLSLEE146 pKa = 4.43EE147 pKa = 5.04TIEE150 pKa = 3.85TWPAEE155 pKa = 4.28EE156 pKa = 4.43VFGIYY161 pKa = 10.03YY162 pKa = 10.41DD163 pKa = 4.97GYY165 pKa = 10.81RR166 pKa = 11.84SWGTNDD172 pKa = 2.75TGIYY176 pKa = 9.88YY177 pKa = 10.2LGNGGGPIRR186 pKa = 11.84IEE188 pKa = 5.0LYY190 pKa = 10.13DD191 pKa = 3.95QEE193 pKa = 4.29ILGYY197 pKa = 7.66TVSVYY202 pKa = 10.59VSGKK206 pKa = 8.45TEE208 pKa = 4.34TITPMRR214 pKa = 11.84YY215 pKa = 8.53IDD217 pKa = 3.51SSLNVLSEE225 pKa = 4.71PIDD228 pKa = 3.98PEE230 pKa = 4.22SEE232 pKa = 4.11KK233 pKa = 11.1PEE235 pKa = 3.5IGYY238 pKa = 9.23QVDD241 pKa = 3.67EE242 pKa = 4.66QDD244 pKa = 2.91QKK246 pKa = 11.05MYY248 pKa = 11.06YY249 pKa = 8.2LTEE252 pKa = 4.52EE253 pKa = 3.27IGYY256 pKa = 9.91RR257 pKa = 11.84LAVLDD262 pKa = 4.28AATGSRR268 pKa = 11.84WYY270 pKa = 10.62GLEE273 pKa = 3.75KK274 pKa = 10.55TEE276 pKa = 5.09DD277 pKa = 3.61AGEE280 pKa = 4.2SWVSVNPDD288 pKa = 3.39PFDD291 pKa = 3.73GRR293 pKa = 11.84SGSSTGLKK301 pKa = 10.08FFNEE305 pKa = 4.65DD306 pKa = 3.18FGFMAIARR314 pKa = 11.84RR315 pKa = 11.84DD316 pKa = 3.4QATLFRR322 pKa = 11.84TEE324 pKa = 3.95NGGEE328 pKa = 4.05IISPVTFPDD337 pKa = 3.42VQVPLIDD344 pKa = 4.93DD345 pKa = 3.42EE346 pKa = 4.95TYY348 pKa = 11.09NPFRR352 pKa = 11.84FPDD355 pKa = 3.46MPYY358 pKa = 10.65EE359 pKa = 4.36EE360 pKa = 5.11NDD362 pKa = 3.28EE363 pKa = 4.87LYY365 pKa = 9.61VTVGQDD371 pKa = 3.18PNGDD375 pKa = 3.81YY376 pKa = 11.38NSGAKK381 pKa = 9.89ALYY384 pKa = 10.25KK385 pKa = 10.69SVDD388 pKa = 3.82DD389 pKa = 4.54GKK391 pKa = 8.28TWTYY395 pKa = 10.23VDD397 pKa = 3.56EE398 pKa = 4.55VGIGFGNDD406 pKa = 2.65

Molecular weight: 46.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H6SKJ8|A0A1H6SKJ8_9LACT Transcriptional regulator OS=Alkalibacterium gilvum OX=1130080 GN=SAMN04488113_10714 PE=4 SV=1
MM1 pKa = 7.35AQGLTYY7 pKa = 10.18QPKK10 pKa = 8.23KK11 pKa = 10.01RR12 pKa = 11.84KK13 pKa = 7.66RR14 pKa = 11.84QRR16 pKa = 11.84VHH18 pKa = 6.34GFRR21 pKa = 11.84KK22 pKa = 10.04RR23 pKa = 11.84MSTKK27 pKa = 9.72NGRR30 pKa = 11.84RR31 pKa = 11.84VLKK34 pKa = 9.89NRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 8.47GRR41 pKa = 11.84KK42 pKa = 9.15RR43 pKa = 11.84ISAA46 pKa = 3.72

Molecular weight: 5.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2101

0

2101

615918

39

2328

293.2

33.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.373 ± 0.051

0.441 ± 0.012

5.93 ± 0.053

7.975 ± 0.071

4.419 ± 0.05

6.316 ± 0.045

1.895 ± 0.023

7.849 ± 0.059

7.496 ± 0.053

9.607 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.71 ± 0.024

5.049 ± 0.046

3.202 ± 0.025

3.428 ± 0.03

3.918 ± 0.04

6.332 ± 0.036

5.744 ± 0.035

6.689 ± 0.04

0.859 ± 0.019

3.768 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski