Wenzhou tombus-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KH37|A0A1L3KH37_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 1 OX=1923662 PE=3 SV=1
MM1 pKa = 7.12VSAAFTFGCDD11 pKa = 2.81VGTSILLLVAVVGVVLGVRR30 pKa = 11.84WLRR33 pKa = 11.84SSTTGATLVAEE44 pKa = 4.51VDD46 pKa = 4.67LGPDD50 pKa = 3.06WTSVGVTTPYY60 pKa = 10.36QRR62 pKa = 11.84QLAYY66 pKa = 10.24QFKK69 pKa = 11.11AEE71 pKa = 4.13FGEE74 pKa = 4.25LRR76 pKa = 11.84YY77 pKa = 10.71NKK79 pKa = 10.0ANRR82 pKa = 11.84IIAGDD87 pKa = 3.6WCRR90 pKa = 11.84KK91 pKa = 9.38AMADD95 pKa = 3.15NDD97 pKa = 3.51VRR99 pKa = 11.84LTDD102 pKa = 3.73RR103 pKa = 11.84VRR105 pKa = 11.84LLPLAVEE112 pKa = 4.7LCLLPTRR119 pKa = 11.84HH120 pKa = 5.83AVAAAEE126 pKa = 3.89LAATHH131 pKa = 5.78EE132 pKa = 4.41VRR134 pKa = 11.84HH135 pKa = 5.7RR136 pKa = 11.84RR137 pKa = 11.84AAADD141 pKa = 3.81LPKK144 pKa = 9.71XGCPAILPGVTTRR157 pKa = 11.84VDD159 pKa = 3.51RR160 pKa = 11.84VGEE163 pKa = 3.76ACLRR167 pKa = 11.84VRR169 pKa = 11.84EE170 pKa = 4.23CVGVGVRR177 pKa = 11.84SGVDD181 pKa = 2.86RR182 pKa = 11.84SVRR185 pKa = 11.84YY186 pKa = 7.99MAGFGTGVRR195 pKa = 11.84YY196 pKa = 9.18GVHH199 pKa = 6.34CANLKK204 pKa = 9.66NLARR208 pKa = 11.84GIVEE212 pKa = 4.08RR213 pKa = 11.84VFHH216 pKa = 5.21VVRR219 pKa = 11.84DD220 pKa = 3.9GRR222 pKa = 11.84LAQAPQPRR230 pKa = 11.84FGVFSRR236 pKa = 11.84LNSVRR241 pKa = 11.84QRR243 pKa = 11.84LLAVTRR249 pKa = 11.84PTPVVARR256 pKa = 11.84EE257 pKa = 4.52DD258 pKa = 3.89YY259 pKa = 10.25PGLYY263 pKa = 9.31TGRR266 pKa = 11.84KK267 pKa = 7.19RR268 pKa = 11.84GIYY271 pKa = 9.33EE272 pKa = 3.9RR273 pKa = 11.84ALEE276 pKa = 4.21SLTVRR281 pKa = 11.84AINSRR286 pKa = 11.84DD287 pKa = 2.83AWVNTFVKK295 pKa = 10.53AEE297 pKa = 4.02KK298 pKa = 10.82VNLDD302 pKa = 3.82SKK304 pKa = 11.25GDD306 pKa = 3.66PAPRR310 pKa = 11.84VIQPRR315 pKa = 11.84SPRR318 pKa = 11.84YY319 pKa = 7.87NLEE322 pKa = 3.47VGRR325 pKa = 11.84YY326 pKa = 7.88LKK328 pKa = 10.18MFEE331 pKa = 5.17RR332 pKa = 11.84EE333 pKa = 3.93LCHH336 pKa = 6.7GFEE339 pKa = 4.6RR340 pKa = 11.84VWGYY344 pKa = 9.87PVVLKK349 pKa = 10.79GMNAQQVGGWMAAHH363 pKa = 7.16WGAFRR368 pKa = 11.84RR369 pKa = 11.84PVAVGLDD376 pKa = 3.23ASRR379 pKa = 11.84FDD381 pKa = 3.33QHH383 pKa = 7.36VSYY386 pKa = 11.07DD387 pKa = 3.46ALRR390 pKa = 11.84WEE392 pKa = 4.12HH393 pKa = 6.33SVYY396 pKa = 11.01NSTFHH401 pKa = 7.78SPEE404 pKa = 3.71LAKK407 pKa = 10.7LLRR410 pKa = 11.84WQLHH414 pKa = 4.33NRR416 pKa = 11.84GVARR420 pKa = 11.84TEE422 pKa = 4.41GYY424 pKa = 10.43RR425 pKa = 11.84IDD427 pKa = 3.8YY428 pKa = 9.84DD429 pKa = 3.33IRR431 pKa = 11.84GCRR434 pKa = 11.84MSGDD438 pKa = 3.53INTGMGNCLLMSSMVIAYY456 pKa = 9.09CEE458 pKa = 4.22SVGIEE463 pKa = 3.61YY464 pKa = 10.82RR465 pKa = 11.84LANNGDD471 pKa = 3.67DD472 pKa = 3.37CVLFVEE478 pKa = 4.57QQDD481 pKa = 4.05LVKK484 pKa = 10.93LSGIDD489 pKa = 3.07EE490 pKa = 4.19WMLDD494 pKa = 3.49FGFTLTRR501 pKa = 11.84EE502 pKa = 3.91EE503 pKa = 3.77PAYY506 pKa = 10.54VLEE509 pKa = 4.59HH510 pKa = 7.73VEE512 pKa = 4.54FCQARR517 pKa = 11.84PVFTSTGWRR526 pKa = 11.84MVRR529 pKa = 11.84DD530 pKa = 3.53PRR532 pKa = 11.84VAMSKK537 pKa = 10.67DD538 pKa = 3.69CVSLLGWEE546 pKa = 4.41SDD548 pKa = 3.87LDD550 pKa = 3.79LQYY553 pKa = 10.15WCHH556 pKa = 7.41AIGTCGASLTAGVPVWHH573 pKa = 6.47SWYY576 pKa = 10.3HH577 pKa = 5.22RR578 pKa = 11.84LQRR581 pKa = 11.84MGAVAPSGVVDD592 pKa = 3.57MVYY595 pKa = 10.95DD596 pKa = 3.56SGLGYY601 pKa = 9.75MSRR604 pKa = 11.84GVVGGEE610 pKa = 3.8VCAEE614 pKa = 3.71ARR616 pKa = 11.84VSFWRR621 pKa = 11.84AFGITPDD628 pKa = 3.86LQQSLEE634 pKa = 4.18DD635 pKa = 4.1EE636 pKa = 4.32YY637 pKa = 11.88SEE639 pKa = 4.2AVVVAKK645 pKa = 9.03PCPMTFPDD653 pKa = 4.07VLAIDD658 pKa = 3.81TSEE661 pKa = 4.33NPLATWLVAARR672 pKa = 11.84TPTLL676 pKa = 3.38

Molecular weight: 75.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KH37|A0A1L3KH37_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 1 OX=1923662 PE=3 SV=1
MM1 pKa = 7.32ARR3 pKa = 11.84SRR5 pKa = 11.84KK6 pKa = 9.36NPNPVTGGVRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84TRR21 pKa = 11.84APNMKK26 pKa = 9.68GGEE29 pKa = 3.93AGTIVEE35 pKa = 4.32YY36 pKa = 10.77SAIGATVTTGGTGLANHH53 pKa = 6.45KK54 pKa = 10.01RR55 pKa = 11.84VFIGGSPYY63 pKa = 11.26DD64 pKa = 3.6LTNTVGPSICSYY76 pKa = 11.28YY77 pKa = 9.34STCKK81 pKa = 10.54FIPGTKK87 pKa = 9.22IRR89 pKa = 11.84WEE91 pKa = 3.99PSVSFTTPGRR101 pKa = 11.84VYY103 pKa = 11.05VGFTDD108 pKa = 4.26NPEE111 pKa = 3.98TMTNIQSAATQADD124 pKa = 3.76WNNLVKK130 pKa = 10.89GLGDD134 pKa = 3.7VISFPVWQEE143 pKa = 3.68TEE145 pKa = 3.9INFPTKK151 pKa = 10.3LRR153 pKa = 11.84RR154 pKa = 11.84KK155 pKa = 9.3MFDD158 pKa = 3.07TNSAVSFVDD167 pKa = 3.97VNALDD172 pKa = 4.23RR173 pKa = 11.84SAQIGMFVAVDD184 pKa = 4.11GAPVTTSVGSFWYY197 pKa = 9.45HH198 pKa = 6.75DD199 pKa = 3.52KK200 pKa = 11.44VLVEE204 pKa = 5.07GIQPTLTT211 pKa = 3.35

Molecular weight: 22.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

887

211

676

443.5

49.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.681 ± 0.976

2.255 ± 0.623

5.073 ± 0.385

4.735 ± 0.676

3.608 ± 0.54

8.794 ± 0.553

1.917 ± 0.462

3.157 ± 0.981

2.706 ± 0.744

7.892 ± 1.955

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.48 ± 0.053

3.382 ± 1.099

4.848 ± 0.626

2.593 ± 0.106

8.681 ± 1.202

5.75 ± 0.648

6.539 ± 2.306

11.161 ± 0.576

2.368 ± 0.225

3.269 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski