Wuchang romanomermis nematode virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Lispiviridae; Arlivirus; Wuchang arlivirus

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KN23|A0A1L3KN23_9MONO RNA-directed RNA polymerase L OS=Wuchang romanomermis nematode virus 2 OX=2773460 PE=3 SV=1
MM1 pKa = 8.36SEE3 pKa = 4.05EE4 pKa = 4.54NLNEE8 pKa = 4.06MPNGAEE14 pKa = 4.07AAMKK18 pKa = 10.35ANNLIRR24 pKa = 11.84MTRR27 pKa = 11.84NQSMAADD34 pKa = 4.11LLEE37 pKa = 3.79QQYY40 pKa = 10.59RR41 pKa = 11.84GEE43 pKa = 4.58LDD45 pKa = 4.76PILEE49 pKa = 4.44TPTEE53 pKa = 4.22YY54 pKa = 10.56EE55 pKa = 4.17DD56 pKa = 4.1KK57 pKa = 11.33VDD59 pKa = 3.79VSLQALVAINGKK71 pKa = 9.15LADD74 pKa = 4.08VLNGLQMVTTSTQLIMEE91 pKa = 5.69NIPDD95 pKa = 3.86VKK97 pKa = 10.66DD98 pKa = 3.59GNQMKK103 pKa = 8.75EE104 pKa = 4.17TVLTDD109 pKa = 3.91LKK111 pKa = 11.41AHH113 pKa = 6.58MSQEE117 pKa = 3.69IVALYY122 pKa = 10.68ARR124 pKa = 11.84LSKK127 pKa = 10.6EE128 pKa = 3.63IADD131 pKa = 3.76MKK133 pKa = 10.47QAFIGEE139 pKa = 4.18LQKK142 pKa = 10.48IRR144 pKa = 11.84TEE146 pKa = 3.72IQALKK151 pKa = 8.92GQKK154 pKa = 10.43SMASLSLQPVSQPMVATTSSAVMPTEE180 pKa = 3.52KK181 pKa = 10.37RR182 pKa = 11.84KK183 pKa = 9.92IDD185 pKa = 3.28MRR187 pKa = 11.84EE188 pKa = 3.6YY189 pKa = 11.25SEE191 pKa = 4.17ILSAFRR197 pKa = 11.84NYY199 pKa = 10.15EE200 pKa = 3.61GSDD203 pKa = 3.37ANKK206 pKa = 10.04QAIQAAILRR215 pKa = 11.84RR216 pKa = 11.84DD217 pKa = 3.58LVAARR222 pKa = 11.84AIMNGG227 pKa = 3.18

Molecular weight: 25.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KN03|A0A1L3KN03_9MONO Uncharacterized protein OS=Wuchang romanomermis nematode virus 2 OX=2773460 PE=4 SV=1
MM1 pKa = 7.94DD2 pKa = 5.38RR3 pKa = 11.84PTFTRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84SSSVGPTIEE19 pKa = 3.95SRR21 pKa = 11.84PTVPCKK27 pKa = 10.16YY28 pKa = 9.63WEE30 pKa = 4.78DD31 pKa = 3.16SHH33 pKa = 8.68ICMNGASCRR42 pKa = 11.84FAHH45 pKa = 6.79NDD47 pKa = 3.45YY48 pKa = 11.26DD49 pKa = 4.16PTPLDD54 pKa = 3.21IVRR57 pKa = 11.84HH58 pKa = 5.55LDD60 pKa = 3.51FMVGIMNKK68 pKa = 6.92ITEE71 pKa = 4.69RR72 pKa = 11.84ISRR75 pKa = 11.84LEE77 pKa = 3.82MKK79 pKa = 10.65VSGRR83 pKa = 11.84SNFQQQGTLQQRR95 pKa = 11.84ASRR98 pKa = 11.84MLAHH102 pKa = 7.05SNRR105 pKa = 11.84FDD107 pKa = 3.28RR108 pKa = 11.84RR109 pKa = 11.84SRR111 pKa = 11.84YY112 pKa = 8.74HH113 pKa = 7.04ASPSSAVSRR122 pKa = 11.84MSQRR126 pKa = 11.84DD127 pKa = 3.79EE128 pKa = 4.63LDD130 pKa = 2.94SSQPVQTSFRR140 pKa = 11.84TEE142 pKa = 3.52PHH144 pKa = 6.79HH145 pKa = 7.25NPFSPDD151 pKa = 2.92LMGQFTPQIPSFSYY165 pKa = 8.56QQAWEE170 pKa = 4.24MPPKK174 pKa = 10.52PFVNKK179 pKa = 8.43NTQIAKK185 pKa = 10.21KK186 pKa = 9.88IAEE189 pKa = 4.16EE190 pKa = 3.83KK191 pKa = 9.25QANIKK196 pKa = 10.74AMLEE200 pKa = 4.03LEE202 pKa = 4.87KK203 pKa = 10.68RR204 pKa = 11.84LQNNLDD210 pKa = 3.29QATVKK215 pKa = 10.68NVLKK219 pKa = 10.77NAGPSTDD226 pKa = 3.44SQIDD230 pKa = 3.46SDD232 pKa = 4.0KK233 pKa = 11.07KK234 pKa = 10.18QEE236 pKa = 4.03KK237 pKa = 10.38EE238 pKa = 3.86DD239 pKa = 3.76MKK241 pKa = 11.26QEE243 pKa = 4.25GQSEE247 pKa = 4.33FF248 pKa = 3.67

Molecular weight: 28.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3045

129

2058

609.0

69.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.568 ± 0.858

1.609 ± 0.325

5.156 ± 0.257

5.846 ± 0.428

4.302 ± 0.589

4.039 ± 0.233

2.266 ± 0.354

7.98 ± 0.889

5.944 ± 0.143

10.608 ± 1.179

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.791 ± 0.797

4.532 ± 0.475

4.532 ± 0.542

4.368 ± 0.98

5.09 ± 0.598

7.258 ± 0.717

6.305 ± 0.321

5.484 ± 0.286

1.478 ± 0.423

3.842 ± 0.642
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski