Vibrio phage pVco-5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 126 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JUR2|A0A1W6JUR2_9CAUD Uncharacterized protein OS=Vibrio phage pVco-5 OX=1965485 GN=pVco5_020 PE=4 SV=1
MM1 pKa = 7.13THH3 pKa = 6.26LTDD6 pKa = 5.54EE7 pKa = 4.7IFDD10 pKa = 3.95TAKK13 pKa = 9.97TYY15 pKa = 11.35SNSHH19 pKa = 7.25PDD21 pKa = 3.63GQPMLEE27 pKa = 4.31EE28 pKa = 4.61LKK30 pKa = 10.68QCIADD35 pKa = 3.59GWSMCMYY42 pKa = 10.27IDD44 pKa = 4.39YY45 pKa = 10.54LSQYY49 pKa = 7.85FTYY52 pKa = 11.07NSIQPIIEE60 pKa = 5.17PIWDD64 pKa = 3.75MEE66 pKa = 4.25LTGANSS72 pKa = 3.08

Molecular weight: 8.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JUU7|A0A1W6JUU7_9CAUD DNA-directed RNA polymerase OS=Vibrio phage pVco-5 OX=1965485 GN=pVco5_061 PE=3 SV=1
MM1 pKa = 7.03QLISTFVWEE10 pKa = 4.53DD11 pKa = 4.15DD12 pKa = 2.91IHH14 pKa = 6.88GRR16 pKa = 11.84PKK18 pKa = 10.65LNYY21 pKa = 8.93RR22 pKa = 11.84NEE24 pKa = 4.07GVYY27 pKa = 10.79LNSIVGLKK35 pKa = 10.02RR36 pKa = 11.84RR37 pKa = 11.84MGGRR41 pKa = 11.84PYY43 pKa = 10.48KK44 pKa = 9.82IHH46 pKa = 6.71RR47 pKa = 11.84TRR49 pKa = 11.84KK50 pKa = 8.04YY51 pKa = 10.46LSRR54 pKa = 11.84SS55 pKa = 3.29

Molecular weight: 6.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

126

0

126

22960

37

3095

182.2

20.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.857 ± 0.478

0.849 ± 0.14

6.233 ± 0.245

6.825 ± 0.409

3.645 ± 0.154

5.828 ± 0.283

2.047 ± 0.176

6.084 ± 0.258

6.982 ± 0.254

8.114 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.966 ± 0.16

5.54 ± 0.18

3.667 ± 0.161

4.111 ± 0.228

4.299 ± 0.187

6.172 ± 0.19

6.89 ± 0.273

6.533 ± 0.247

1.093 ± 0.097

4.264 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski