Pseudomonas phage Bjorn

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Bjornvirus; Pseudomonas virus Bjorn

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9VHF4|A0A2K9VHF4_9CAUD Scaffold protein OS=Pseudomonas phage Bjorn OX=2079288 GN=PsPhBjorn_gp66 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 9.91RR3 pKa = 11.84DD4 pKa = 3.33EE5 pKa = 5.02DD6 pKa = 3.92VCSNCGAEE14 pKa = 4.26LEE16 pKa = 4.44EE17 pKa = 4.58PQAQGVFAEE26 pKa = 4.66CLSCAVDD33 pKa = 4.49VVDD36 pKa = 4.81EE37 pKa = 4.42DD38 pKa = 3.58WLAGVSCNPNAPEE51 pKa = 4.16EE52 pKa = 4.49CEE54 pKa = 4.23SCQQ57 pKa = 4.26

Molecular weight: 6.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9VHI2|A0A2K9VHI2_9CAUD Lysozyme OS=Pseudomonas phage Bjorn OX=2079288 GN=PsPhBjorn_gp03 PE=4 SV=1
MM1 pKa = 7.64TIAKK5 pKa = 9.11DD6 pKa = 3.23AKK8 pKa = 10.22LRR10 pKa = 11.84RR11 pKa = 11.84NMRR14 pKa = 11.84NQARR18 pKa = 11.84RR19 pKa = 11.84NGDD22 pKa = 3.27PMGLLNNNQGGTVDD36 pKa = 4.77NLLATYY42 pKa = 10.2KK43 pKa = 10.44RR44 pKa = 11.84ARR46 pKa = 11.84DD47 pKa = 3.74RR48 pKa = 11.84NSLLQALSKK57 pKa = 10.43IRR59 pKa = 11.84NTDD62 pKa = 3.09PQAQRR67 pKa = 11.84LYY69 pKa = 10.94KK70 pKa = 10.39ALQNHH75 pKa = 6.25WYY77 pKa = 10.17NRR79 pKa = 11.84TEE81 pKa = 3.91HH82 pKa = 5.62VQEE85 pKa = 4.22FMVDD89 pKa = 3.62GKK91 pKa = 10.03PVTRR95 pKa = 11.84GKK97 pKa = 10.33EE98 pKa = 3.68KK99 pKa = 9.14QTRR102 pKa = 11.84KK103 pKa = 9.8SSPLIRR109 pKa = 11.84SFYY112 pKa = 11.03

Molecular weight: 13.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

14193

37

1066

205.7

22.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.66 ± 0.542

1.029 ± 0.132

6.383 ± 0.247

6.017 ± 0.339

3.431 ± 0.182

8.342 ± 0.427

1.945 ± 0.182

4.756 ± 0.201

5.425 ± 0.338

8.37 ± 0.322

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.811 ± 0.197

4.65 ± 0.291

4.523 ± 0.247

4.488 ± 0.31

5.517 ± 0.352

5.228 ± 0.272

5.763 ± 0.264

6.778 ± 0.297

1.592 ± 0.135

3.29 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski