Lactobacillus phage Ld25A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cequinquevirus; Lactobacillus virus Ld25A

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075KK25|A0A075KK25_9CAUD Uncharacterized protein OS=Lactobacillus phage Ld25A OX=1500734 GN=LDB25A_016 PE=4 SV=1
MM1 pKa = 7.32NSEE4 pKa = 4.22LEE6 pKa = 4.29KK7 pKa = 10.38IFKK10 pKa = 10.62NFTVNGKK17 pKa = 9.29QIPVSFLRR25 pKa = 11.84YY26 pKa = 9.39DD27 pKa = 3.77GKK29 pKa = 8.81EE30 pKa = 3.85TTYY33 pKa = 9.28ITYY36 pKa = 10.14QEE38 pKa = 4.02IMVDD42 pKa = 3.89EE43 pKa = 4.67VFSADD48 pKa = 4.2DD49 pKa = 4.49EE50 pKa = 4.53IVSYY54 pKa = 10.79SDD56 pKa = 5.23CYY58 pKa = 11.6DD59 pKa = 3.29FDD61 pKa = 4.35IYY63 pKa = 11.58SKK65 pKa = 11.33GNYY68 pKa = 8.75FPIVEE73 pKa = 4.19AVKK76 pKa = 10.61EE77 pKa = 3.88ILKK80 pKa = 11.04ANDD83 pKa = 3.09WVYY86 pKa = 11.41NPQNNSGDD94 pKa = 3.75LYY96 pKa = 10.99EE97 pKa = 6.17DD98 pKa = 3.35EE99 pKa = 4.52TGLYY103 pKa = 10.34HH104 pKa = 6.37KK105 pKa = 8.71TLNFIHH111 pKa = 6.77IRR113 pKa = 3.63

Molecular weight: 13.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075KJA3|A0A075KJA3_9CAUD Putative tail component OS=Lactobacillus phage Ld25A OX=1500734 GN=LDB25A_015 PE=4 SV=1
MM1 pKa = 7.68AKK3 pKa = 10.36FEE5 pKa = 4.57MEE7 pKa = 4.25LPDD10 pKa = 6.57DD11 pKa = 3.66IFKK14 pKa = 11.07DD15 pKa = 3.46VTFVEE20 pKa = 4.65KK21 pKa = 10.49RR22 pKa = 11.84ARR24 pKa = 11.84TVFGGMTKK32 pKa = 10.52AGAEE36 pKa = 4.07YY37 pKa = 10.57VRR39 pKa = 11.84NEE41 pKa = 4.53VISGMPAGMKK51 pKa = 10.09KK52 pKa = 10.33AGLASGVKK60 pKa = 7.32VTRR63 pKa = 11.84TYY65 pKa = 8.75RR66 pKa = 11.84TPSDD70 pKa = 3.86DD71 pKa = 4.21GINNKK76 pKa = 8.67VVMGGYY82 pKa = 7.57FTNEE86 pKa = 4.03NGVKK90 pKa = 9.64TPIPLVANLFEE101 pKa = 4.73YY102 pKa = 10.81GSSRR106 pKa = 11.84ANYY109 pKa = 7.37PKK111 pKa = 10.39QPFFRR116 pKa = 11.84KK117 pKa = 9.53AFKK120 pKa = 10.37KK121 pKa = 10.71SEE123 pKa = 3.64IEE125 pKa = 4.01AIMRR129 pKa = 11.84AKK131 pKa = 10.2QKK133 pKa = 10.09EE134 pKa = 4.18LSGGLLGDD142 pKa = 3.9EE143 pKa = 4.56

Molecular weight: 15.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

10037

50

968

196.8

22.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.785 ± 0.584

0.917 ± 0.154

6.606 ± 0.389

6.785 ± 0.463

3.955 ± 0.166

6.944 ± 0.37

1.365 ± 0.163

6.885 ± 0.286

8.638 ± 0.481

7.662 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.361 ± 0.146

6.625 ± 0.287

2.451 ± 0.166

3.328 ± 0.235

4.015 ± 0.249

6.655 ± 0.284

5.848 ± 0.336

6.984 ± 0.208

1.046 ± 0.111

4.145 ± 0.31
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski