Gracilibacillus orientalis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Gracilibacillus

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4278 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4LTD3|A0A1I4LTD3_9BACI Drug resistance transporter EmrB/QacA subfamily OS=Gracilibacillus orientalis OX=334253 GN=SAMN04487943_105200 PE=3 SV=1
MM1 pKa = 7.6KK2 pKa = 10.25KK3 pKa = 9.68ILLIAILFTMSVVIMACSSDD23 pKa = 3.46EE24 pKa = 4.06ADD26 pKa = 3.33SVEE29 pKa = 4.39EE30 pKa = 3.93NVEE33 pKa = 4.05SDD35 pKa = 4.28GEE37 pKa = 4.36TEE39 pKa = 4.04EE40 pKa = 5.55QSDD43 pKa = 3.85GDD45 pKa = 4.13EE46 pKa = 3.92NTLYY50 pKa = 10.93DD51 pKa = 4.09EE52 pKa = 5.32SNLLSLGQSGSIEE65 pKa = 4.23ATNSSYY71 pKa = 11.2EE72 pKa = 3.93VTVEE76 pKa = 3.98SFEE79 pKa = 4.12VLNDD83 pKa = 3.24IVGQQSSEE91 pKa = 3.81EE92 pKa = 4.25VYY94 pKa = 10.7ILLDD98 pKa = 3.61YY99 pKa = 11.06QITNTGEE106 pKa = 3.69EE107 pKa = 4.61AIQGLAVTEE116 pKa = 4.33GNLFNAAGAKK126 pKa = 9.17GLSVTSYY133 pKa = 11.4DD134 pKa = 4.43FISIPDD140 pKa = 3.83TEE142 pKa = 4.39IKK144 pKa = 10.28PGEE147 pKa = 4.15SAAAQILFEE156 pKa = 4.14QEE158 pKa = 4.02EE159 pKa = 4.22SDD161 pKa = 3.49EE162 pKa = 4.6YY163 pKa = 11.75YY164 pKa = 11.25DD165 pKa = 4.36FIYY168 pKa = 10.98NFGSSDD174 pKa = 3.8GEE176 pKa = 4.33LVWRR180 pKa = 11.84LNADD184 pKa = 3.39EE185 pKa = 5.01AANEE189 pKa = 4.06FDD191 pKa = 3.99GQLTQEE197 pKa = 5.1DD198 pKa = 4.75IEE200 pKa = 4.42TLKK203 pKa = 10.89GGEE206 pKa = 3.98MSEE209 pKa = 3.98FAQNIEE215 pKa = 3.94VLEE218 pKa = 4.27VEE220 pKa = 4.6KK221 pKa = 10.38VTHH224 pKa = 6.27NGEE227 pKa = 4.24EE228 pKa = 4.23IEE230 pKa = 4.7FPTDD234 pKa = 3.23QEE236 pKa = 4.08IMIEE240 pKa = 4.27SEE242 pKa = 4.19GEE244 pKa = 4.12VYY246 pKa = 10.26IDD248 pKa = 3.41SDD250 pKa = 4.56FITTVADD257 pKa = 3.53YY258 pKa = 10.81EE259 pKa = 4.24LTYY262 pKa = 10.93DD263 pKa = 4.13EE264 pKa = 4.65EE265 pKa = 4.28NQYY268 pKa = 10.89VEE270 pKa = 4.59VIEE273 pKa = 4.84GKK275 pKa = 10.57ADD277 pKa = 3.72FQFEE281 pKa = 4.29PQFDD285 pKa = 4.21EE286 pKa = 4.16NDD288 pKa = 3.47GGIIEE293 pKa = 4.32VNEE296 pKa = 4.5FYY298 pKa = 10.75FDD300 pKa = 3.83EE301 pKa = 4.49NDD303 pKa = 3.43EE304 pKa = 4.45YY305 pKa = 10.86ISRR308 pKa = 11.84SDD310 pKa = 3.57YY311 pKa = 10.6KK312 pKa = 11.22AEE314 pKa = 4.03SQEE317 pKa = 4.04LYY319 pKa = 11.33NLIEE323 pKa = 4.48YY324 pKa = 9.69KK325 pKa = 10.39NHH327 pKa = 6.02VYY329 pKa = 10.67LSYY332 pKa = 11.06AITNDD337 pKa = 3.36FLKK340 pKa = 10.15EE341 pKa = 3.96TVNYY345 pKa = 8.45NQSEE349 pKa = 4.61STVEE353 pKa = 3.31IGEE356 pKa = 4.15NSKK359 pKa = 10.75SKK361 pKa = 10.15QVSEE365 pKa = 4.34ILHH368 pKa = 6.76DD369 pKa = 4.11SGGPKK374 pKa = 10.09AKK376 pKa = 10.49EE377 pKa = 3.6NVTIQGEE384 pKa = 4.52KK385 pKa = 10.03YY386 pKa = 10.51DD387 pKa = 3.91GAIDD391 pKa = 3.93FDD393 pKa = 4.59DD394 pKa = 3.84VGSFTEE400 pKa = 4.3YY401 pKa = 9.58VWLNGEE407 pKa = 4.06HH408 pKa = 7.05KK409 pKa = 10.32YY410 pKa = 10.73SHH412 pKa = 7.04FNGSIYY418 pKa = 10.84YY419 pKa = 8.59KK420 pKa = 9.9TDD422 pKa = 3.1SNLTLTVSFVKK433 pKa = 10.54NDD435 pKa = 3.2NNQTVIKK442 pKa = 9.87EE443 pKa = 4.09FEE445 pKa = 4.3LEE447 pKa = 4.0PGEE450 pKa = 4.27MKK452 pKa = 10.75DD453 pKa = 3.73VDD455 pKa = 3.24IDD457 pKa = 3.64FSGEE461 pKa = 3.43HH462 pKa = 5.48FMGVFSKK469 pKa = 10.66QAIGNNTGGQLLVIGEE485 pKa = 4.34VKK487 pKa = 10.64

Molecular weight: 54.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4KL54|A0A1I4KL54_9BACI Transcriptional regulator BadM/Rrf2 family OS=Gracilibacillus orientalis OX=334253 GN=SAMN04487943_10414 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.12KK14 pKa = 8.46VHH16 pKa = 5.5GFRR19 pKa = 11.84TRR21 pKa = 11.84MSTKK25 pKa = 9.9NGKK28 pKa = 9.88KK29 pKa = 9.59IIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.39KK37 pKa = 10.08GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4278

0

4278

1301525

39

3190

304.2

34.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.419 ± 0.037

0.633 ± 0.011

5.737 ± 0.04

7.77 ± 0.059

4.596 ± 0.035

6.448 ± 0.039

2.149 ± 0.02

8.131 ± 0.042

6.47 ± 0.033

9.438 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.726 ± 0.016

4.862 ± 0.033

3.473 ± 0.022

4.161 ± 0.029

3.627 ± 0.026

6.042 ± 0.032

5.584 ± 0.026

6.813 ± 0.033

1.156 ± 0.016

3.764 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski