Bacillus phage BSP10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Nitunavirus; unclassified Nitunavirus

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I6UHR8|A0A2I6UHR8_9CAUD Uncharacterized protein OS=Bacillus phage BSP10 OX=2069312 GN=BSP10_135 PE=4 SV=1
MM1 pKa = 7.39SFFFEE6 pKa = 5.28GCLQDD11 pKa = 5.0NVIFYY16 pKa = 10.49DD17 pKa = 4.33AGDD20 pKa = 3.61ILIGGVSATYY30 pKa = 10.5VIVKK34 pKa = 9.44EE35 pKa = 4.3KK36 pKa = 10.72YY37 pKa = 8.19RR38 pKa = 11.84NEE40 pKa = 3.93WSSDD44 pKa = 3.0PVAILTDD51 pKa = 3.57SFEE54 pKa = 4.17EE55 pKa = 4.12VQDD58 pKa = 3.86FEE60 pKa = 5.17EE61 pKa = 4.49VVNMFF66 pKa = 4.09

Molecular weight: 7.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I6UI06|A0A2I6UI06_9CAUD Uncharacterized protein OS=Bacillus phage BSP10 OX=2069312 GN=BSP10_188 PE=4 SV=1
MM1 pKa = 7.46ARR3 pKa = 11.84GRR5 pKa = 11.84KK6 pKa = 9.19SIRR9 pKa = 11.84PKK11 pKa = 10.0MFQSKK16 pKa = 10.08AATQRR21 pKa = 11.84AMNNFGNALIQRR33 pKa = 11.84TLSAGEE39 pKa = 3.78EE40 pKa = 4.25AVRR43 pKa = 11.84NAQPNDD49 pKa = 3.15VSVTRR54 pKa = 11.84KK55 pKa = 9.41PKK57 pKa = 9.66YY58 pKa = 10.66LEE60 pKa = 3.8VTEE63 pKa = 3.92NRR65 pKa = 11.84LRR67 pKa = 11.84KK68 pKa = 10.05LGVIDD73 pKa = 5.27LKK75 pKa = 11.26PFFAKK80 pKa = 10.24SSKK83 pKa = 10.84KK84 pKa = 10.19KK85 pKa = 8.77MKK87 pKa = 10.43KK88 pKa = 9.68GGGWYY93 pKa = 8.62LTVPITRR100 pKa = 11.84KK101 pKa = 6.87TRR103 pKa = 11.84NMSRR107 pKa = 11.84KK108 pKa = 8.98MYY110 pKa = 10.29DD111 pKa = 2.85QLRR114 pKa = 11.84SVNTRR119 pKa = 11.84PNTKK123 pKa = 8.62KK124 pKa = 9.07TVITDD129 pKa = 3.15YY130 pKa = 11.07LYY132 pKa = 10.94DD133 pKa = 3.61RR134 pKa = 11.84RR135 pKa = 11.84QISDD139 pKa = 3.39STMLNYY145 pKa = 10.36NPRR148 pKa = 11.84SKK150 pKa = 10.96NITKK154 pKa = 10.15EE155 pKa = 3.77RR156 pKa = 11.84VGKK159 pKa = 9.67NRR161 pKa = 11.84HH162 pKa = 5.89SYY164 pKa = 8.95VSYY167 pKa = 9.08RR168 pKa = 11.84TVSDD172 pKa = 3.73KK173 pKa = 11.5SPANSWIINRR183 pKa = 11.84GKK185 pKa = 10.75VNEE188 pKa = 4.42ADD190 pKa = 3.4TSKK193 pKa = 9.79TFIKK197 pKa = 10.82NVDD200 pKa = 3.36RR201 pKa = 11.84LIRR204 pKa = 11.84WQMKK208 pKa = 9.98NNN210 pKa = 3.52

Molecular weight: 24.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

236

0

236

45117

37

1391

191.2

21.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.075 ± 0.217

0.813 ± 0.076

6.563 ± 0.15

8.303 ± 0.284

3.87 ± 0.128

6.335 ± 0.225

1.875 ± 0.084

6.095 ± 0.145

7.611 ± 0.233

8.336 ± 0.172

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.62 ± 0.086

5.202 ± 0.159

3.221 ± 0.167

3.619 ± 0.135

4.542 ± 0.137

6.321 ± 0.188

6.166 ± 0.199

6.913 ± 0.151

1.04 ± 0.062

4.479 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski