Mycobacterium phage Arissanae

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481W4C5|A0A481W4C5_9CAUD Uncharacterized protein OS=Mycobacterium phage Arissanae OX=2530173 GN=84 PE=4 SV=1
MM1 pKa = 7.48TKK3 pKa = 10.11TITRR7 pKa = 11.84MLLTIVAGGAALGAMALADD26 pKa = 4.25PYY28 pKa = 10.26EE29 pKa = 4.49ASSTTGTVDD38 pKa = 3.24GVDD41 pKa = 3.52YY42 pKa = 9.1PYY44 pKa = 11.1CSLEE48 pKa = 4.38DD49 pKa = 4.46CSDD52 pKa = 3.28QPGQVGIWIDD62 pKa = 3.81RR63 pKa = 11.84DD64 pKa = 3.62TGNQWLSLGEE74 pKa = 3.96TSYY77 pKa = 11.36LIQRR81 pKa = 4.25

Molecular weight: 8.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481W494|A0A481W494_9CAUD DNA polymerase I OS=Mycobacterium phage Arissanae OX=2530173 GN=44 PE=3 SV=1
MM1 pKa = 7.35SWADD5 pKa = 2.94SDD7 pKa = 4.61RR8 pKa = 11.84RR9 pKa = 11.84QNLPADD15 pKa = 3.59WEE17 pKa = 4.24QRR19 pKa = 11.84RR20 pKa = 11.84LAVLVDD26 pKa = 3.85AEE28 pKa = 4.35WLCEE32 pKa = 3.89IEE34 pKa = 4.21MAGCTRR40 pKa = 11.84VATDD44 pKa = 2.9VDD46 pKa = 3.91HH47 pKa = 7.0VKK49 pKa = 10.57RR50 pKa = 11.84GNDD53 pKa = 2.94HH54 pKa = 5.92SRR56 pKa = 11.84RR57 pKa = 11.84NLRR60 pKa = 11.84AACGWCHH67 pKa = 6.24DD68 pKa = 4.39RR69 pKa = 11.84KK70 pKa = 10.78SSAEE74 pKa = 3.67GVARR78 pKa = 11.84RR79 pKa = 11.84RR80 pKa = 11.84EE81 pKa = 3.88LKK83 pKa = 10.28ARR85 pKa = 11.84RR86 pKa = 11.84KK87 pKa = 9.71RR88 pKa = 11.84PPEE91 pKa = 3.6RR92 pKa = 11.84HH93 pKa = 5.7PGRR96 pKa = 11.84RR97 pKa = 3.38

Molecular weight: 11.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

16606

41

970

176.7

19.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.267 ± 0.398

0.807 ± 0.104

6.257 ± 0.207

6.672 ± 0.316

3.439 ± 0.175

8.34 ± 0.355

1.801 ± 0.168

5.07 ± 0.18

4.348 ± 0.198

8.184 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.644 ± 0.177

3.475 ± 0.155

5.209 ± 0.221

3.529 ± 0.208

6.449 ± 0.271

5.649 ± 0.248

6.154 ± 0.247

6.997 ± 0.225

1.915 ± 0.119

2.794 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski