Ralstonia pickettii (strain 12J)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Ralstonia; Ralstonia pickettii

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4890 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2U777|B2U777_RALPJ TfoX domain protein OS=Ralstonia pickettii (strain 12J) OX=402626 GN=Rpic_3659 PE=4 SV=1
MM1 pKa = 7.15EE2 pKa = 3.93QQIEE6 pKa = 4.59YY7 pKa = 8.1KK8 pKa = 9.59TWMCLICGWIYY19 pKa = 11.03DD20 pKa = 4.25EE21 pKa = 4.67EE22 pKa = 4.34QGAPDD27 pKa = 4.95DD28 pKa = 5.22GIAPGTKK35 pKa = 9.07WEE37 pKa = 4.52DD38 pKa = 3.42VPINWTCPEE47 pKa = 3.97CGARR51 pKa = 11.84KK52 pKa = 9.54EE53 pKa = 4.07DD54 pKa = 3.87FEE56 pKa = 4.52MVQII60 pKa = 4.59

Molecular weight: 7.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2U855|B2U855_RALPJ Purine nucleoside permease OS=Ralstonia pickettii (strain 12J) OX=402626 GN=Rpic_0746 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.72GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4890

0

4890

1540967

24

2556

315.1

34.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.828 ± 0.045

0.923 ± 0.015

5.348 ± 0.023

5.084 ± 0.029

3.534 ± 0.022

8.129 ± 0.034

2.363 ± 0.02

4.602 ± 0.028

3.212 ± 0.029

10.275 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.502 ± 0.015

2.802 ± 0.022

5.213 ± 0.024

3.867 ± 0.023

6.873 ± 0.036

5.312 ± 0.026

5.585 ± 0.026

7.736 ± 0.031

1.426 ± 0.017

2.388 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski