Echinacea purpurea witches -broom phytoplasma

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae; Candidatus Phytoplasma; 16SrII (Peanut WB group)

Average proteome isoelectric point is 8.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 502 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P7HXU3|A0A0P7HXU3_9MOLU ABC-type spermidine/putrescine transport system periplasmic protein OS='Echinacea purpurea' witches'-broom phytoplasma OX=1123738 GN=potD PE=4 SV=1
MM1 pKa = 7.7INQSNNQNAFKK12 pKa = 9.64EE13 pKa = 4.13YY14 pKa = 11.17SMLEE18 pKa = 3.58IALDD22 pKa = 3.63ILQKK26 pKa = 10.57NPKK29 pKa = 8.84PMPVYY34 pKa = 10.46QLIEE38 pKa = 3.84QVLDD42 pKa = 3.23IKK44 pKa = 10.91KK45 pKa = 9.92IEE47 pKa = 4.35NLRR50 pKa = 11.84QDD52 pKa = 4.38CFLQLYY58 pKa = 9.77LDD60 pKa = 3.87IVISGLFVFHH70 pKa = 7.45GEE72 pKa = 4.17DD73 pKa = 2.89LWGIKK78 pKa = 8.72TDD80 pKa = 6.12NLDD83 pKa = 3.51LWDD86 pKa = 3.85KK87 pKa = 10.82EE88 pKa = 4.41YY89 pKa = 11.38YY90 pKa = 10.47ADD92 pKa = 4.59EE93 pKa = 4.5EE94 pKa = 4.77KK95 pKa = 11.07SDD97 pKa = 4.0IDD99 pKa = 5.53NEE101 pKa = 4.13LLDD104 pKa = 4.94LKK106 pKa = 11.03YY107 pKa = 11.19SNNLDD112 pKa = 3.36DD113 pKa = 6.49DD114 pKa = 4.28FRR116 pKa = 11.84LEE118 pKa = 3.88NDD120 pKa = 3.06SDD122 pKa = 4.13FSHH125 pKa = 7.04TDD127 pKa = 2.87EE128 pKa = 4.35TADD131 pKa = 4.61RR132 pKa = 11.84EE133 pKa = 4.19ILEE136 pKa = 5.6DD137 pKa = 3.58IDD139 pKa = 5.66DD140 pKa = 4.09VVLGDD145 pKa = 4.29DD146 pKa = 3.58EE147 pKa = 5.06AVLRR151 pKa = 11.84EE152 pKa = 4.1EE153 pKa = 4.25SDD155 pKa = 3.68SVNAEE160 pKa = 3.53EE161 pKa = 5.92DD162 pKa = 3.41KK163 pKa = 11.75NEE165 pKa = 5.62DD166 pKa = 3.1INEE169 pKa = 4.0YY170 pKa = 10.54DD171 pKa = 3.11WAPNEE176 pKa = 4.11EE177 pKa = 4.23

Molecular weight: 20.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P7JJ83|A0A0P7JJ83_9MOLU Putative effector AYWB SAP68-like protein OS='Echinacea purpurea' witches'-broom phytoplasma OX=1123738 GN=EPWB_v1c4000 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.17QPSKK9 pKa = 9.44IKK11 pKa = 10.44RR12 pKa = 11.84KK13 pKa = 8.23RR14 pKa = 11.84THH16 pKa = 6.39GFLKK20 pKa = 10.52RR21 pKa = 11.84MSNASGRR28 pKa = 11.84RR29 pKa = 11.84LLSNRR34 pKa = 11.84RR35 pKa = 11.84YY36 pKa = 10.01KK37 pKa = 10.63GRR39 pKa = 11.84HH40 pKa = 4.12QLTVV44 pKa = 2.92

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

502

0

502

147945

31

2293

294.7

34.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.765 ± 0.104

1.011 ± 0.037

4.822 ± 0.119

5.662 ± 0.137

5.777 ± 0.128

3.899 ± 0.119

1.887 ± 0.058

11.477 ± 0.116

10.392 ± 0.124

11.015 ± 0.111

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.884 ± 0.038

8.396 ± 0.128

2.618 ± 0.065

4.764 ± 0.129

3.035 ± 0.067

6.011 ± 0.121

4.417 ± 0.153

4.261 ± 0.096

0.603 ± 0.029

4.304 ± 0.077
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski