Amino acid dipepetide frequency for Plasmodium falciparum RAJ116

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	0.917AlaAla: 0.917 ± 0.04	0.518AlaCys: 0.518 ± 0.02	1.13AlaAsp: 1.13 ± 0.033	1.307AlaGlu: 1.307 ± 0.043	0.893AlaPhe: 0.893 ± 0.026	0.765AlaGly: 0.765 ± 0.028	0.585AlaHis: 0.585 ± 0.019	1.506AlaIle: 1.506 ± 0.036	1.731AlaLys: 1.731 ± 0.043	1.763AlaLeu: 1.763 ± 0.037	0.383AlaMet: 0.383 ± 0.017	1.618AlaAsn: 1.618 ± 0.037	0.611AlaPro: 0.611 ± 0.028	0.711AlaGln: 0.711 ± 0.019	0.579AlaArg: 0.579 ± 0.025	1.49AlaSer: 1.49 ± 0.038	0.974AlaThr: 0.974 ± 0.033	0.837AlaVal: 0.837 ± 0.026	0.132AlaTrp: 0.132 ± 0.009	1.085AlaTyr: 1.085 ± 0.026	0.0AlaXaa: 0.0 ± 0.0
Cys	0.53CysAla: 0.53 ± 0.018	0.303CysCys: 0.303 ± 0.014	1.392CysAsp: 1.392 ± 0.036	1.185CysGlu: 1.185 ± 0.032	0.777CysPhe: 0.777 ± 0.022	0.767CysGly: 0.767 ± 0.03	0.323CysHis: 0.323 ± 0.014	1.656CysIle: 1.656 ± 0.03	1.686CysLys: 1.686 ± 0.049	1.444CysLeu: 1.444 ± 0.03	0.364CysMet: 0.364 ± 0.016	1.905CysAsn: 1.905 ± 0.036	0.5CysPro: 0.5 ± 0.025	0.357CysGln: 0.357 ± 0.017	0.538CysArg: 0.538 ± 0.027	1.434CysSer: 1.434 ± 0.034	1.011CysThr: 1.011 ± 0.03	0.88CysVal: 0.88 ± 0.023	0.059CysTrp: 0.059 ± 0.005	0.794CysTyr: 0.794 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	1.289AspAla: 1.289 ± 0.033	0.775AspCys: 0.775 ± 0.031	7.57AspAsp: 7.57 ± 0.149	6.035AspGlu: 6.035 ± 0.089	2.062AspPhe: 2.062 ± 0.033	1.962AspGly: 1.962 ± 0.043	1.615AspHis: 1.615 ± 0.035	7.409AspIle: 7.409 ± 0.075	7.004AspLys: 7.004 ± 0.088	3.65AspLeu: 3.65 ± 0.053	1.775AspMet: 1.775 ± 0.035	9.531AspAsn: 9.531 ± 0.126	1.084AspPro: 1.084 ± 0.034	1.539AspGln: 1.539 ± 0.041	1.248AspArg: 1.248 ± 0.038	3.312AspSer: 3.312 ± 0.05	2.902AspThr: 2.902 ± 0.046	3.111AspVal: 3.111 ± 0.057	0.256AspTrp: 0.256 ± 0.014	2.901AspTyr: 2.901 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	1.476GluAla: 1.476 ± 0.04	1.117GluCys: 1.117 ± 0.028	4.995GluAsp: 4.995 ± 0.09	9.345GluGlu: 9.345 ± 0.409	2.029GluPhe: 2.029 ± 0.04	2.284GluGly: 2.284 ± 0.064	1.899GluHis: 1.899 ± 0.044	5.33GluIle: 5.33 ± 0.115	10.84GluLys: 10.84 ± 0.125	4.6GluLeu: 4.6 ± 0.104	1.456GluMet: 1.456 ± 0.034	9.101GluAsn: 9.101 ± 0.111	1.107GluPro: 1.107 ± 0.039	2.721GluGln: 2.721 ± 0.056	2.163GluArg: 2.163 ± 0.047	3.426GluSer: 3.426 ± 0.069	2.479GluThr: 2.479 ± 0.045	2.634GluVal: 2.634 ± 0.199	0.601GluTrp: 0.601 ± 0.029	3.657GluTyr: 3.657 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	0.766PheAla: 0.766 ± 0.024	0.926PheCys: 0.926 ± 0.026	2.376PheAsp: 2.376 ± 0.038	2.251PheGlu: 2.251 ± 0.039	3.066PhePhe: 3.066 ± 0.064	1.182PheGly: 1.182 ± 0.033	1.076PheHis: 1.076 ± 0.023	3.923PheIle: 3.923 ± 0.06	3.448PheLys: 3.448 ± 0.045	4.57PheLeu: 4.57 ± 0.062	0.87PheMet: 0.87 ± 0.019	4.096PheAsn: 4.096 ± 0.056	0.969PhePro: 0.969 ± 0.023	1.025PheGln: 1.025 ± 0.025	0.961PheArg: 0.961 ± 0.021	3.055PheSer: 3.055 ± 0.05	1.504PheThr: 1.504 ± 0.035	1.835PheVal: 1.835 ± 0.035	0.205PheTrp: 0.205 ± 0.01	2.599PheTyr: 2.599 ± 0.056	0.0PheXaa: 0.0 ± 0.0
Gly	0.87GlyAla: 0.87 ± 0.035	0.535GlyCys: 0.535 ± 0.021	2.596GlyAsp: 2.596 ± 0.061	2.093GlyGlu: 2.093 ± 0.05	1.034GlyPhe: 1.034 ± 0.027	1.697GlyGly: 1.697 ± 0.061	0.644GlyHis: 0.644 ± 0.019	2.376GlyIle: 2.376 ± 0.046	3.295GlyLys: 3.295 ± 0.059	1.964GlyLeu: 1.964 ± 0.052	0.633GlyMet: 0.633 ± 0.02	3.345GlyAsn: 3.345 ± 0.054	0.702GlyPro: 0.702 ± 0.032	0.765GlyGln: 0.765 ± 0.025	0.955GlyArg: 0.955 ± 0.026	2.157GlySer: 2.157 ± 0.066	1.635GlyThr: 1.635 ± 0.045	1.367GlyVal: 1.367 ± 0.029	0.175GlyTrp: 0.175 ± 0.01	1.451GlyTyr: 1.451 ± 0.032	0.0GlyXaa: 0.0 ± 0.0
His	0.462HisAla: 0.462 ± 0.018	0.296HisCys: 0.296 ± 0.013	1.484HisAsp: 1.484 ± 0.039	1.319HisGlu: 1.319 ± 0.031	1.211HisPhe: 1.211 ± 0.023	0.658HisGly: 0.658 ± 0.023	0.85HisHis: 0.85 ± 0.042	2.794HisIle: 2.794 ± 0.045	2.32HisLys: 2.32 ± 0.042	1.835HisLeu: 1.835 ± 0.033	0.902HisMet: 0.902 ± 0.025	3.576HisAsn: 3.576 ± 0.073	0.584HisPro: 0.584 ± 0.015	0.546HisGln: 0.546 ± 0.018	0.548HisArg: 0.548 ± 0.018	1.423HisSer: 1.423 ± 0.032	1.188HisThr: 1.188 ± 0.033	1.282HisVal: 1.282 ± 0.044	0.103HisTrp: 0.103 ± 0.008	1.081HisTyr: 1.081 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	1.38IleAla: 1.38 ± 0.035	2.0IleCys: 2.0 ± 0.043	4.7IleAsp: 4.7 ± 0.057	5.159IleGlu: 5.159 ± 0.074	4.273IlePhe: 4.273 ± 0.067	2.232IleGly: 2.232 ± 0.039	2.573IleHis: 2.573 ± 0.043	7.8IleIle: 7.8 ± 0.103	10.194IleLys: 10.194 ± 0.11	7.62IleLeu: 7.62 ± 0.084	1.645IleMet: 1.645 ± 0.032	12.627IleAsn: 12.627 ± 0.159	2.659IlePro: 2.659 ± 0.09	2.88IleGln: 2.88 ± 0.054	2.237IleArg: 2.237 ± 0.039	5.996IleSer: 5.996 ± 0.066	3.399IleThr: 3.399 ± 0.054	2.748IleVal: 2.748 ± 0.059	0.474IleTrp: 0.474 ± 0.017	6.114IleTyr: 6.114 ± 0.086	0.0IleXaa: 0.0 ± 0.0
Lys	1.828LysAla: 1.828 ± 0.048	2.167LysCys: 2.167 ± 0.059	7.159LysAsp: 7.159 ± 0.086	10.381LysGlu: 10.381 ± 0.133	3.0LysPhe: 3.0 ± 0.041	3.621LysGly: 3.621 ± 0.053	2.347LysHis: 2.347 ± 0.044	9.102LysIle: 9.102 ± 0.094	19.677LysLys: 19.677 ± 0.235	7.1LysLeu: 7.1 ± 0.076	2.64LysMet: 2.64 ± 0.047	16.846LysAsn: 16.846 ± 0.142	1.56LysPro: 1.56 ± 0.036	3.121LysGln: 3.121 ± 0.057	4.208LysArg: 4.208 ± 0.052	6.292LysSer: 6.292 ± 0.072	4.25LysThr: 4.25 ± 0.061	3.329LysVal: 3.329 ± 0.052	0.701LysTrp: 0.701 ± 0.025	6.751LysTyr: 6.751 ± 0.085	0.0LysXaa: 0.0 ± 0.0
Leu	1.447LeuAla: 1.447 ± 0.035	1.739LeuCys: 1.739 ± 0.036	3.555LeuAsp: 3.555 ± 0.049	4.386LeuGlu: 4.386 ± 0.074	4.001LeuPhe: 4.001 ± 0.059	2.006LeuGly: 2.006 ± 0.04	1.816LeuHis: 1.816 ± 0.03	5.68LeuIle: 5.68 ± 0.069	8.681LeuLys: 8.681 ± 0.083	6.926LeuLeu: 6.926 ± 0.094	1.259LeuMet: 1.259 ± 0.028	8.546LeuAsn: 8.546 ± 0.094	1.799LeuPro: 1.799 ± 0.041	2.284LeuGln: 2.284 ± 0.037	2.333LeuArg: 2.333 ± 0.047	5.34LeuSer: 5.34 ± 0.063	2.727LeuThr: 2.727 ± 0.043	2.374LeuVal: 2.374 ± 0.072	0.439LeuTrp: 0.439 ± 0.016	4.622LeuTyr: 4.622 ± 0.07	0.0LeuXaa: 0.0 ± 0.0
Met	0.367MetAla: 0.367 ± 0.015	0.484MetCys: 0.484 ± 0.017	1.681MetAsp: 1.681 ± 0.039	1.561MetGlu: 1.561 ± 0.035	0.829MetPhe: 0.829 ± 0.023	0.615MetGly: 0.615 ± 0.028	0.439MetHis: 0.439 ± 0.018	1.527MetIle: 1.527 ± 0.032	2.945MetLys: 2.945 ± 0.051	1.666MetLeu: 1.666 ± 0.033	0.523MetMet: 0.523 ± 0.022	4.255MetAsn: 4.255 ± 0.111	0.413MetPro: 0.413 ± 0.018	0.526MetGln: 0.526 ± 0.016	0.572MetArg: 0.572 ± 0.017	1.494MetSer: 1.494 ± 0.034	0.694MetThr: 0.694 ± 0.02	0.726MetVal: 0.726 ± 0.022	0.117MetTrp: 0.117 ± 0.007	1.159MetTyr: 1.159 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	1.984AsnAla: 1.984 ± 0.046	1.603AsnCys: 1.603 ± 0.031	10.456AsnAsp: 10.456 ± 0.138	9.809AsnGlu: 9.809 ± 0.117	4.544AsnPhe: 4.544 ± 0.06	3.374AsnGly: 3.374 ± 0.056	2.898AsnHis: 2.898 ± 0.061	15.889AsnIle: 15.889 ± 0.169	15.526AsnLys: 15.526 ± 0.168	7.463AsnLeu: 7.463 ± 0.061	4.349AsnMet: 4.349 ± 0.113	33.788AsnAsn: 33.788 ± 0.739	1.762AsnPro: 1.762 ± 0.039	2.909AsnGln: 2.909 ± 0.055	2.567AsnArg: 2.567 ± 0.04	7.861AsnSer: 7.861 ± 0.099	5.651AsnThr: 5.651 ± 0.07	6.454AsnVal: 6.454 ± 0.093	0.337AsnTrp: 0.337 ± 0.015	7.002AsnTyr: 7.002 ± 0.122	0.0AsnXaa: 0.0 ± 0.0
Pro	0.498ProAla: 0.498 ± 0.023	0.477ProCys: 0.477 ± 0.023	0.868ProAsp: 0.868 ± 0.036	1.447ProGlu: 1.447 ± 0.14	1.229ProPhe: 1.229 ± 0.029	0.593ProGly: 0.593 ± 0.024	0.532ProHis: 0.532 ± 0.015	1.703ProIle: 1.703 ± 0.041	1.816ProLys: 1.816 ± 0.042	1.85ProLeu: 1.85 ± 0.037	0.403ProMet: 0.403 ± 0.017	2.239ProAsn: 2.239 ± 0.055	0.946ProPro: 0.946 ± 0.043	0.735ProGln: 0.735 ± 0.028	0.605ProArg: 0.605 ± 0.019	1.751ProSer: 1.751 ± 0.04	1.078ProThr: 1.078 ± 0.034	0.808ProVal: 0.808 ± 0.027	0.135ProTrp: 0.135 ± 0.009	1.336ProTyr: 1.336 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	0.587GlnAla: 0.587 ± 0.017	0.434GlnCys: 0.434 ± 0.016	1.442GlnAsp: 1.442 ± 0.041	1.999GlnGlu: 1.999 ± 0.052	0.946GlnPhe: 0.946 ± 0.024	0.805GlnGly: 0.805 ± 0.024	0.766GlnHis: 0.766 ± 0.028	2.38GlnIle: 2.38 ± 0.044	3.585GlnLys: 3.585 ± 0.056	1.848GlnLeu: 1.848 ± 0.038	0.729GlnMet: 0.729 ± 0.026	4.326GlnAsn: 4.326 ± 0.081	0.591GlnPro: 0.591 ± 0.022	1.172GlnGln: 1.172 ± 0.05	0.824GlnArg: 0.824 ± 0.022	1.506GlnSer: 1.506 ± 0.035	1.32GlnThr: 1.32 ± 0.033	0.918GlnVal: 0.918 ± 0.025	0.175GlnTrp: 0.175 ± 0.01	1.374GlnTyr: 1.374 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	0.648ArgAla: 0.648 ± 0.021	0.427ArgCys: 0.427 ± 0.015	1.603ArgAsp: 1.603 ± 0.048	1.788ArgGlu: 1.788 ± 0.041	0.923ArgPhe: 0.923 ± 0.023	1.062ArgGly: 1.062 ± 0.028	0.588ArgHis: 0.588 ± 0.016	2.071ArgIle: 2.071 ± 0.033	3.892ArgLys: 3.892 ± 0.061	1.734ArgLeu: 1.734 ± 0.045	0.537ArgMet: 0.537 ± 0.018	3.484ArgAsn: 3.484 ± 0.052	0.478ArgPro: 0.478 ± 0.019	0.724ArgGln: 0.724 ± 0.019	1.474ArgArg: 1.474 ± 0.045	1.655ArgSer: 1.655 ± 0.034	1.145ArgThr: 1.145 ± 0.027	0.883ArgVal: 0.883 ± 0.023	0.246ArgTrp: 0.246 ± 0.015	1.325ArgTyr: 1.325 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	1.382SerAla: 1.382 ± 0.034	1.252SerCys: 1.252 ± 0.026	4.254SerAsp: 4.254 ± 0.067	3.618SerGlu: 3.618 ± 0.06	3.371SerPhe: 3.371 ± 0.047	2.335SerGly: 2.335 ± 0.084	1.627SerHis: 1.627 ± 0.044	5.326SerIle: 5.326 ± 0.062	5.607SerLys: 5.607 ± 0.061	5.116SerLeu: 5.116 ± 0.062	1.224SerMet: 1.224 ± 0.029	8.447SerAsn: 8.447 ± 0.109	1.443SerPro: 1.443 ± 0.041	1.669SerGln: 1.669 ± 0.035	1.595SerArg: 1.595 ± 0.037	6.932SerSer: 6.932 ± 0.118	3.199SerThr: 3.199 ± 0.049	2.695SerVal: 2.695 ± 0.071	0.278SerTrp: 0.278 ± 0.012	3.696SerTyr: 3.696 ± 0.053	0.0SerXaa: 0.0 ± 0.0
Thr	0.914ThrAla: 0.914 ± 0.027	1.057ThrCys: 1.057 ± 0.031	2.121ThrAsp: 2.121 ± 0.042	2.212ThrGlu: 2.212 ± 0.089	2.096ThrPhe: 2.096 ± 0.035	1.173ThrGly: 1.173 ± 0.035	1.272ThrHis: 1.272 ± 0.032	3.017ThrIle: 3.017 ± 0.044	4.127ThrLys: 4.127 ± 0.059	3.231ThrLeu: 3.231 ± 0.05	0.689ThrMet: 0.689 ± 0.02	6.005ThrAsn: 6.005 ± 0.091	1.245ThrPro: 1.245 ± 0.043	1.41ThrGln: 1.41 ± 0.033	0.905ThrArg: 0.905 ± 0.025	3.418ThrSer: 3.418 ± 0.066	2.4ThrThr: 2.4 ± 0.065	1.353ThrVal: 1.353 ± 0.035	0.22ThrTrp: 0.22 ± 0.01	2.748ThrTyr: 2.748 ± 0.048	0.0ThrXaa: 0.0 ± 0.0
Val	0.989ValAla: 0.989 ± 0.035	0.843ValCys: 0.843 ± 0.023	2.806ValAsp: 2.806 ± 0.052	3.201ValGlu: 3.201 ± 0.165	1.428ValPhe: 1.428 ± 0.033	1.366ValGly: 1.366 ± 0.037	1.224ValHis: 1.224 ± 0.028	3.022ValIle: 3.022 ± 0.088	3.572ValLys: 3.572 ± 0.048	3.142ValLeu: 3.142 ± 0.047	0.679ValMet: 0.679 ± 0.018	4.149ValAsn: 4.149 ± 0.072	1.354ValPro: 1.354 ± 0.067	1.385ValGln: 1.385 ± 0.03	0.978ValArg: 0.978 ± 0.023	2.839ValSer: 2.839 ± 0.084	1.648ValThr: 1.648 ± 0.056	1.988ValVal: 1.988 ± 0.114	0.254ValTrp: 0.254 ± 0.013	1.906ValTyr: 1.906 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	0.155TrpAla: 0.155 ± 0.011	0.105TrpCys: 0.105 ± 0.006	0.31TrpAsp: 0.31 ± 0.014	0.375TrpGlu: 0.375 ± 0.018	0.274TrpPhe: 0.274 ± 0.013	0.235TrpGly: 0.235 ± 0.011	0.084TrpHis: 0.084 ± 0.007	0.51TrpIle: 0.51 ± 0.019	0.655TrpLys: 0.655 ± 0.022	0.43TrpLeu: 0.43 ± 0.015	0.105TrpMet: 0.105 ± 0.008	0.563TrpAsn: 0.563 ± 0.021	0.09TrpPro: 0.09 ± 0.007	0.096TrpGln: 0.096 ± 0.007	0.172TrpArg: 0.172 ± 0.009	0.312TrpSer: 0.312 ± 0.015	0.211TrpThr: 0.211 ± 0.011	0.239TrpVal: 0.239 ± 0.01	0.114TrpTrp: 0.114 ± 0.011	0.206TrpTyr: 0.206 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.046TyrAla: 1.046 ± 0.027	0.847TyrCys: 0.847 ± 0.027	4.535TyrAsp: 4.535 ± 0.057	3.786TyrGlu: 3.786 ± 0.049	2.715TyrPhe: 2.715 ± 0.053	1.556TyrGly: 1.556 ± 0.036	1.304TyrHis: 1.304 ± 0.03	5.689TyrIle: 5.689 ± 0.085	5.479TyrLys: 5.479 ± 0.07	4.01TyrLeu: 4.01 ± 0.055	1.428TyrMet: 1.428 ± 0.033	7.78TyrAsn: 7.78 ± 0.121	1.15TyrPro: 1.15 ± 0.026	1.149TyrGln: 1.149 ± 0.028	1.224TyrArg: 1.224 ± 0.026	3.374TyrSer: 3.374 ± 0.051	2.199TyrThr: 2.199 ± 0.035	2.314TyrVal: 2.314 ± 0.037	0.221TyrTrp: 0.221 ± 0.011	3.28TyrTyr: 3.28 ± 0.057	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3177 proteins (2165978 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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