Mycobacterium phage MissWhite

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A249XTB0|A0A249XTB0_9CAUD Uncharacterized protein OS=Mycobacterium phage MissWhite OX=2024297 GN=61 PE=4 SV=1
MM1 pKa = 7.41AQRR4 pKa = 11.84ATAINMQDD12 pKa = 3.24RR13 pKa = 11.84FIVCAGEE20 pKa = 4.09PMLDD24 pKa = 3.28TDD26 pKa = 4.19EE27 pKa = 4.7GVLIIQFEE35 pKa = 4.6DD36 pKa = 3.3GTNRR40 pKa = 11.84IFNWDD45 pKa = 3.94YY46 pKa = 11.06IIDD49 pKa = 4.04YY50 pKa = 11.11YY51 pKa = 11.85YY52 pKa = 8.52MTEE55 pKa = 4.05DD56 pKa = 3.14

Molecular weight: 6.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A249XTB9|A0A249XTB9_9CAUD Glutaredoxin OS=Mycobacterium phage MissWhite OX=2024297 GN=57 PE=4 SV=1
MM1 pKa = 7.3KK2 pKa = 9.91WGVRR6 pKa = 11.84YY7 pKa = 8.69PRR9 pKa = 11.84SGIHH13 pKa = 5.54EE14 pKa = 4.47CPFGFKK20 pKa = 9.1QASMIEE26 pKa = 3.89LLAIRR31 pKa = 11.84NGVRR35 pKa = 11.84AQLVSSSDD43 pKa = 3.53GGQTWVTRR51 pKa = 3.96

Molecular weight: 5.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

15603

24

835

169.6

18.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.876 ± 0.316

0.987 ± 0.115

6.614 ± 0.224

7.088 ± 0.288

3.448 ± 0.192

8.197 ± 0.393

2.07 ± 0.172

4.986 ± 0.179

4.384 ± 0.263

7.973 ± 0.397

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.615 ± 0.165

3.358 ± 0.185

5.287 ± 0.227

3.583 ± 0.186

6.588 ± 0.438

5.012 ± 0.217

5.992 ± 0.302

7.095 ± 0.224

1.974 ± 0.146

2.871 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski