Amino acid dipepetide frequency for Thermoanaerobacteraceae bacterium SP2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.872AlaAla: 6.872 ± 0.107	0.886AlaCys: 0.886 ± 0.039	3.537AlaAsp: 3.537 ± 0.063	4.894AlaGlu: 4.894 ± 0.068	3.075AlaPhe: 3.075 ± 0.067	6.455AlaGly: 6.455 ± 0.107	1.151AlaHis: 1.151 ± 0.034	5.959AlaIle: 5.959 ± 0.096	4.569AlaLys: 4.569 ± 0.074	7.794AlaLeu: 7.794 ± 0.101	2.241AlaMet: 2.241 ± 0.051	2.203AlaAsn: 2.203 ± 0.046	2.175AlaPro: 2.175 ± 0.059	2.144AlaGln: 2.144 ± 0.051	4.189AlaArg: 4.189 ± 0.072	3.791AlaSer: 3.791 ± 0.07	3.101AlaThr: 3.101 ± 0.051	6.44AlaVal: 6.44 ± 0.091	0.544AlaTrp: 0.544 ± 0.023	2.136AlaTyr: 2.136 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.727CysAla: 0.727 ± 0.025	0.176CysCys: 0.176 ± 0.014	0.596CysAsp: 0.596 ± 0.025	0.587CysGlu: 0.587 ± 0.023	0.414CysPhe: 0.414 ± 0.021	1.188CysGly: 1.188 ± 0.045	0.259CysHis: 0.259 ± 0.018	0.849CysIle: 0.849 ± 0.029	0.647CysLys: 0.647 ± 0.028	0.83CysLeu: 0.83 ± 0.031	0.263CysMet: 0.263 ± 0.016	0.429CysAsn: 0.429 ± 0.021	0.638CysPro: 0.638 ± 0.027	0.212CysGln: 0.212 ± 0.014	0.568CysArg: 0.568 ± 0.025	0.665CysSer: 0.665 ± 0.026	0.442CysThr: 0.442 ± 0.021	0.599CysVal: 0.599 ± 0.027	0.095CysTrp: 0.095 ± 0.009	0.354CysTyr: 0.354 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	3.513AspAla: 3.513 ± 0.073	0.467AspCys: 0.467 ± 0.023	2.524AspAsp: 2.524 ± 0.057	4.318AspGlu: 4.318 ± 0.068	2.73AspPhe: 2.73 ± 0.058	3.683AspGly: 3.683 ± 0.079	0.703AspHis: 0.703 ± 0.024	5.655AspIle: 5.655 ± 0.077	3.569AspLys: 3.569 ± 0.066	4.947AspLeu: 4.947 ± 0.077	1.678AspMet: 1.678 ± 0.041	1.947AspAsn: 1.947 ± 0.05	2.148AspPro: 2.148 ± 0.046	0.949AspGln: 0.949 ± 0.031	2.596AspArg: 2.596 ± 0.056	2.516AspSer: 2.516 ± 0.054	2.389AspThr: 2.389 ± 0.056	4.178AspVal: 4.178 ± 0.066	0.411AspTrp: 0.411 ± 0.022	2.117AspTyr: 2.117 ± 0.05	0.0AspXaa: 0.0 ± 0.0
Glu	5.301GluAla: 5.301 ± 0.091	0.574GluCys: 0.574 ± 0.023	3.724GluAsp: 3.724 ± 0.066	5.999GluGlu: 5.999 ± 0.094	2.539GluPhe: 2.539 ± 0.055	4.541GluGly: 4.541 ± 0.079	1.06GluHis: 1.06 ± 0.036	6.543GluIle: 6.543 ± 0.101	7.329GluLys: 7.329 ± 0.099	6.657GluLeu: 6.657 ± 0.097	2.155GluMet: 2.155 ± 0.047	3.689GluAsn: 3.689 ± 0.062	2.058GluPro: 2.058 ± 0.045	2.215GluGln: 2.215 ± 0.053	3.4GluArg: 3.4 ± 0.059	3.272GluSer: 3.272 ± 0.061	2.932GluThr: 2.932 ± 0.054	4.841GluVal: 4.841 ± 0.073	0.516GluTrp: 0.516 ± 0.022	2.304GluTyr: 2.304 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	2.834PheAla: 2.834 ± 0.054	0.515PheCys: 0.515 ± 0.023	2.362PheAsp: 2.362 ± 0.055	2.639PheGlu: 2.639 ± 0.053	2.038PhePhe: 2.038 ± 0.054	3.224PheGly: 3.224 ± 0.056	0.699PheHis: 0.699 ± 0.024	3.647PheIle: 3.647 ± 0.066	2.79PheLys: 2.79 ± 0.049	4.099PheLeu: 4.099 ± 0.079	1.184PheMet: 1.184 ± 0.032	1.948PheAsn: 1.948 ± 0.042	1.651PhePro: 1.651 ± 0.048	1.029PheGln: 1.029 ± 0.034	1.618PheArg: 1.618 ± 0.042	2.822PheSer: 2.822 ± 0.052	2.255PheThr: 2.255 ± 0.047	2.507PheVal: 2.507 ± 0.056	0.333PheTrp: 0.333 ± 0.017	1.639PheTyr: 1.639 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	5.532GlyAla: 5.532 ± 0.098	1.052GlyCys: 1.052 ± 0.032	3.414GlyAsp: 3.414 ± 0.06	4.885GlyGlu: 4.885 ± 0.067	3.598GlyPhe: 3.598 ± 0.066	5.72GlyGly: 5.72 ± 0.113	1.334GlyHis: 1.334 ± 0.037	7.407GlyIle: 7.407 ± 0.103	5.671GlyLys: 5.671 ± 0.077	6.861GlyLeu: 6.861 ± 0.102	2.22GlyMet: 2.22 ± 0.052	2.895GlyAsn: 2.895 ± 0.059	2.246GlyPro: 2.246 ± 0.051	2.03GlyGln: 2.03 ± 0.045	3.652GlyArg: 3.652 ± 0.061	3.927GlySer: 3.927 ± 0.073	3.863GlyThr: 3.863 ± 0.074	5.468GlyVal: 5.468 ± 0.077	0.664GlyTrp: 0.664 ± 0.025	2.858GlyTyr: 2.858 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	1.049HisAla: 1.049 ± 0.033	0.2HisCys: 0.2 ± 0.013	0.749HisAsp: 0.749 ± 0.028	0.98HisGlu: 0.98 ± 0.03	0.748HisPhe: 0.748 ± 0.028	1.2HisGly: 1.2 ± 0.036	0.376HisHis: 0.376 ± 0.021	1.384HisIle: 1.384 ± 0.036	0.939HisLys: 0.939 ± 0.033	1.518HisLeu: 1.518 ± 0.04	0.463HisMet: 0.463 ± 0.022	0.554HisAsn: 0.554 ± 0.024	0.928HisPro: 0.928 ± 0.027	0.46HisGln: 0.46 ± 0.02	0.803HisArg: 0.803 ± 0.03	0.82HisSer: 0.82 ± 0.03	0.719HisThr: 0.719 ± 0.026	1.016HisVal: 1.016 ± 0.035	0.149HisTrp: 0.149 ± 0.013	0.572HisTyr: 0.572 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.231IleAla: 6.231 ± 0.079	0.941IleCys: 0.941 ± 0.031	4.94IleAsp: 4.94 ± 0.079	5.739IleGlu: 5.739 ± 0.083	3.619IlePhe: 3.619 ± 0.071	5.789IleGly: 5.789 ± 0.08	1.324IleHis: 1.324 ± 0.039	7.424IleIle: 7.424 ± 0.115	7.097IleLys: 7.097 ± 0.099	8.31IleLeu: 8.31 ± 0.101	2.283IleMet: 2.283 ± 0.049	4.154IleAsn: 4.154 ± 0.072	3.978IlePro: 3.978 ± 0.067	2.134IleGln: 2.134 ± 0.05	3.497IleArg: 3.497 ± 0.063	5.524IleSer: 5.524 ± 0.077	4.651IleThr: 4.651 ± 0.07	5.438IleVal: 5.438 ± 0.089	0.56IleTrp: 0.56 ± 0.021	2.909IleTyr: 2.909 ± 0.06	0.0IleXaa: 0.0 ± 0.0
Lys	5.673LysAla: 5.673 ± 0.08	0.571LysCys: 0.571 ± 0.027	4.511LysAsp: 4.511 ± 0.072	5.947LysGlu: 5.947 ± 0.095	2.349LysPhe: 2.349 ± 0.047	5.028LysGly: 5.028 ± 0.08	0.969LysHis: 0.969 ± 0.029	6.506LysIle: 6.506 ± 0.082	6.763LysLys: 6.763 ± 0.084	6.262LysLeu: 6.262 ± 0.077	2.197LysMet: 2.197 ± 0.044	4.387LysAsn: 4.387 ± 0.073	2.803LysPro: 2.803 ± 0.062	2.276LysGln: 2.276 ± 0.05	3.108LysArg: 3.108 ± 0.065	3.864LysSer: 3.864 ± 0.069	3.776LysThr: 3.776 ± 0.062	5.324LysVal: 5.324 ± 0.083	0.538LysTrp: 0.538 ± 0.03	2.611LysTyr: 2.611 ± 0.058	0.0LysXaa: 0.0 ± 0.0
Leu	7.214LeuAla: 7.214 ± 0.099	0.977LeuCys: 0.977 ± 0.03	4.884LeuAsp: 4.884 ± 0.077	6.875LeuGlu: 6.875 ± 0.103	3.696LeuPhe: 3.696 ± 0.08	7.042LeuGly: 7.042 ± 0.098	1.254LeuHis: 1.254 ± 0.037	7.082LeuIle: 7.082 ± 0.107	8.556LeuLys: 8.556 ± 0.095	8.546LeuLeu: 8.546 ± 0.111	2.733LeuMet: 2.733 ± 0.055	4.276LeuAsn: 4.276 ± 0.076	3.867LeuPro: 3.867 ± 0.073	2.456LeuGln: 2.456 ± 0.052	4.027LeuArg: 4.027 ± 0.074	6.545LeuSer: 6.545 ± 0.091	4.548LeuThr: 4.548 ± 0.066	5.998LeuVal: 5.998 ± 0.09	0.72LeuTrp: 0.72 ± 0.026	2.756LeuTyr: 2.756 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	2.748MetAla: 2.748 ± 0.046	0.216MetCys: 0.216 ± 0.016	1.695MetAsp: 1.695 ± 0.044	2.322MetGlu: 2.322 ± 0.043	0.895MetPhe: 0.895 ± 0.03	2.323MetGly: 2.323 ± 0.051	0.377MetHis: 0.377 ± 0.018	2.089MetIle: 2.089 ± 0.045	2.209MetLys: 2.209 ± 0.044	2.653MetLeu: 2.653 ± 0.052	0.844MetMet: 0.844 ± 0.035	1.223MetAsn: 1.223 ± 0.036	1.196MetPro: 1.196 ± 0.033	0.739MetGln: 0.739 ± 0.03	1.163MetArg: 1.163 ± 0.039	1.514MetSer: 1.514 ± 0.036	1.381MetThr: 1.381 ± 0.031	2.411MetVal: 2.411 ± 0.05	0.177MetTrp: 0.177 ± 0.014	0.658MetTyr: 0.658 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	2.75AsnAla: 2.75 ± 0.052	0.5AsnCys: 0.5 ± 0.022	1.871AsnAsp: 1.871 ± 0.047	2.438AsnGlu: 2.438 ± 0.05	1.941AsnPhe: 1.941 ± 0.044	2.755AsnGly: 2.755 ± 0.054	0.666AsnHis: 0.666 ± 0.026	4.636AsnIle: 4.636 ± 0.083	2.759AsnLys: 2.759 ± 0.062	4.56AsnLeu: 4.56 ± 0.067	1.309AsnMet: 1.309 ± 0.037	1.908AsnAsn: 1.908 ± 0.045	2.456AsnPro: 2.456 ± 0.052	1.196AsnGln: 1.196 ± 0.036	1.933AsnArg: 1.933 ± 0.042	2.187AsnSer: 2.187 ± 0.055	2.16AsnThr: 2.16 ± 0.048	2.886AsnVal: 2.886 ± 0.054	0.361AsnTrp: 0.361 ± 0.019	1.64AsnTyr: 1.64 ± 0.039	0.0AsnXaa: 0.0 ± 0.0
Pro	2.858ProAla: 2.858 ± 0.059	0.368ProCys: 0.368 ± 0.019	2.473ProAsp: 2.473 ± 0.047	3.749ProGlu: 3.749 ± 0.06	1.729ProPhe: 1.729 ± 0.04	3.588ProGly: 3.588 ± 0.074	0.735ProHis: 0.735 ± 0.028	2.687ProIle: 2.687 ± 0.057	2.006ProLys: 2.006 ± 0.046	3.513ProLeu: 3.513 ± 0.067	1.0ProMet: 1.0 ± 0.032	1.247ProAsn: 1.247 ± 0.04	1.428ProPro: 1.428 ± 0.045	1.283ProGln: 1.283 ± 0.036	1.618ProArg: 1.618 ± 0.042	2.02ProSer: 2.02 ± 0.045	1.765ProThr: 1.765 ± 0.041	3.5ProVal: 3.5 ± 0.067	0.386ProTrp: 0.386 ± 0.02	1.47ProTyr: 1.47 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	2.118GlnAla: 2.118 ± 0.047	0.199GlnCys: 0.199 ± 0.014	1.581GlnAsp: 1.581 ± 0.042	2.357GlnGlu: 2.357 ± 0.051	0.841GlnPhe: 0.841 ± 0.031	1.875GlnGly: 1.875 ± 0.052	0.392GlnHis: 0.392 ± 0.019	2.241GlnIle: 2.241 ± 0.05	2.794GlnLys: 2.794 ± 0.054	2.39GlnLeu: 2.39 ± 0.048	0.814GlnMet: 0.814 ± 0.032	1.491GlnAsn: 1.491 ± 0.04	0.844GlnPro: 0.844 ± 0.029	1.148GlnGln: 1.148 ± 0.046	1.284GlnArg: 1.284 ± 0.037	1.228GlnSer: 1.228 ± 0.038	1.16GlnThr: 1.16 ± 0.034	1.99GlnVal: 1.99 ± 0.05	0.246GlnTrp: 0.246 ± 0.016	0.808GlnTyr: 0.808 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	3.259ArgAla: 3.259 ± 0.055	0.516ArgCys: 0.516 ± 0.025	2.421ArgAsp: 2.421 ± 0.051	4.099ArgGlu: 4.099 ± 0.069	2.001ArgPhe: 2.001 ± 0.046	3.171ArgGly: 3.171 ± 0.058	0.779ArgHis: 0.779 ± 0.027	4.002ArgIle: 4.002 ± 0.072	3.444ArgLys: 3.444 ± 0.066	4.308ArgLeu: 4.308 ± 0.066	1.456ArgMet: 1.456 ± 0.035	1.849ArgAsn: 1.849 ± 0.043	1.561ArgPro: 1.561 ± 0.044	1.755ArgGln: 1.755 ± 0.042	2.314ArgArg: 2.314 ± 0.057	2.049ArgSer: 2.049 ± 0.044	1.932ArgThr: 1.932 ± 0.044	3.326ArgVal: 3.326 ± 0.062	0.439ArgTrp: 0.439 ± 0.02	1.576ArgTyr: 1.576 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	3.756SerAla: 3.756 ± 0.06	0.63SerCys: 0.63 ± 0.025	2.772SerAsp: 2.772 ± 0.057	3.377SerGlu: 3.377 ± 0.058	2.542SerPhe: 2.542 ± 0.053	4.828SerGly: 4.828 ± 0.079	0.984SerHis: 0.984 ± 0.033	4.855SerIle: 4.855 ± 0.078	3.47SerLys: 3.47 ± 0.066	5.49SerLeu: 5.49 ± 0.085	1.65SerMet: 1.65 ± 0.04	1.983SerAsn: 1.983 ± 0.045	2.444SerPro: 2.444 ± 0.044	1.699SerGln: 1.699 ± 0.044	2.998SerArg: 2.998 ± 0.051	3.533SerSer: 3.533 ± 0.07	2.717SerThr: 2.717 ± 0.054	3.58SerVal: 3.58 ± 0.06	0.464SerTrp: 0.464 ± 0.024	1.749SerTyr: 1.749 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	3.826ThrAla: 3.826 ± 0.079	0.499ThrCys: 0.499 ± 0.024	2.363ThrAsp: 2.363 ± 0.057	2.64ThrGlu: 2.64 ± 0.057	2.071ThrPhe: 2.071 ± 0.044	4.712ThrGly: 4.712 ± 0.076	0.841ThrHis: 0.841 ± 0.03	3.848ThrIle: 3.848 ± 0.075	2.474ThrLys: 2.474 ± 0.046	4.716ThrLeu: 4.716 ± 0.075	1.16ThrMet: 1.16 ± 0.038	1.645ThrAsn: 1.645 ± 0.037	2.34ThrPro: 2.34 ± 0.057	1.265ThrGln: 1.265 ± 0.036	2.278ThrArg: 2.278 ± 0.046	2.613ThrSer: 2.613 ± 0.05	2.396ThrThr: 2.396 ± 0.057	4.195ThrVal: 4.195 ± 0.078	0.384ThrTrp: 0.384 ± 0.021	1.532ThrTyr: 1.532 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	5.318ValAla: 5.318 ± 0.092	0.729ValCys: 0.729 ± 0.03	4.08ValAsp: 4.08 ± 0.067	5.136ValGlu: 5.136 ± 0.084	3.027ValPhe: 3.027 ± 0.059	4.873ValGly: 4.873 ± 0.09	0.991ValHis: 0.991 ± 0.03	6.292ValIle: 6.292 ± 0.084	5.648ValLys: 5.648 ± 0.084	6.769ValLeu: 6.769 ± 0.082	2.059ValMet: 2.059 ± 0.042	3.055ValAsn: 3.055 ± 0.054	2.969ValPro: 2.969 ± 0.055	1.638ValGln: 1.638 ± 0.042	3.125ValArg: 3.125 ± 0.053	4.245ValSer: 4.245 ± 0.071	3.596ValThr: 3.596 ± 0.075	5.606ValVal: 5.606 ± 0.101	0.497ValTrp: 0.497 ± 0.026	2.212ValTyr: 2.212 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.571TrpAla: 0.571 ± 0.026	0.094TrpCys: 0.094 ± 0.01	0.483TrpAsp: 0.483 ± 0.025	0.541TrpGlu: 0.541 ± 0.024	0.366TrpPhe: 0.366 ± 0.02	0.66TrpGly: 0.66 ± 0.023	0.121TrpHis: 0.121 ± 0.011	0.591TrpIle: 0.591 ± 0.025	0.526TrpLys: 0.526 ± 0.024	0.731TrpLeu: 0.731 ± 0.028	0.24TrpMet: 0.24 ± 0.016	0.375TrpAsn: 0.375 ± 0.021	0.298TrpPro: 0.298 ± 0.018	0.332TrpGln: 0.332 ± 0.018	0.343TrpArg: 0.343 ± 0.019	0.412TrpSer: 0.412 ± 0.019	0.321TrpThr: 0.321 ± 0.019	0.544TrpVal: 0.544 ± 0.026	0.11TrpTrp: 0.11 ± 0.012	0.251TrpTyr: 0.251 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.006TyrAla: 2.006 ± 0.044	0.427TyrCys: 0.427 ± 0.022	2.058TyrAsp: 2.058 ± 0.044	2.125TyrGlu: 2.125 ± 0.051	1.615TyrPhe: 1.615 ± 0.045	2.639TyrGly: 2.639 ± 0.059	0.611TyrHis: 0.611 ± 0.028	2.844TyrIle: 2.844 ± 0.054	2.204TyrLys: 2.204 ± 0.047	3.095TyrLeu: 3.095 ± 0.051	0.886TyrMet: 0.886 ± 0.031	1.653TyrAsn: 1.653 ± 0.045	1.395TyrPro: 1.395 ± 0.044	0.842TyrGln: 0.842 ± 0.031	1.746TyrArg: 1.746 ± 0.049	1.975TyrSer: 1.975 ± 0.042	1.693TyrThr: 1.693 ± 0.046	2.019TyrVal: 2.019 ± 0.044	0.316TyrTrp: 0.316 ± 0.016	1.336TyrTyr: 1.336 ± 0.044	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3424 proteins (1012711 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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