Leptospira phage vB_LbrZ_5399-LE1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5VTI7|S5VTI7_9CAUD DNA-directed DNA polymerase OS=Leptospira phage vB_LbrZ_5399-LE1 OX=1334245 GN=LEP1GSC050_0071 PE=3 SV=1
MM1 pKa = 7.44PTFLGPFNFDD11 pKa = 3.03EE12 pKa = 4.41VLEE15 pKa = 4.28KK16 pKa = 11.01SLMSNVVLSEE26 pKa = 4.26LEE28 pKa = 4.11IEE30 pKa = 4.43STLAPGSFNLGEE42 pKa = 3.99ILQVGTLIFQVILSIKK58 pKa = 9.56TPFDD62 pKa = 4.05GNPSLTVGDD71 pKa = 4.22TLTQNRR77 pKa = 11.84FLDD80 pKa = 3.65SSQFSFSEE88 pKa = 3.78QGSYY92 pKa = 10.65FSIPLEE98 pKa = 4.31TIEE101 pKa = 6.06SINQCKK107 pKa = 10.4VYY109 pKa = 9.58WNPGGSSTGSAKK121 pKa = 10.59LYY123 pKa = 10.37LISSNPP129 pKa = 3.19

Molecular weight: 14.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5VYH0|S5VYH0_9CAUD Ankyrin repeat protein OS=Leptospira phage vB_LbrZ_5399-LE1 OX=1334245 GN=LEP1GSC050_0059 PE=4 SV=1
MM1 pKa = 7.75AYY3 pKa = 9.1EE4 pKa = 4.19ALYY7 pKa = 11.07SKK9 pKa = 11.16GKK11 pKa = 9.92FPRR14 pKa = 11.84TKK16 pKa = 10.54YY17 pKa = 10.53VLSQIAKK24 pKa = 8.41EE25 pKa = 4.15AHH27 pKa = 5.42TLWSKK32 pKa = 10.77RR33 pKa = 11.84VLEE36 pKa = 4.25AWPSYY41 pKa = 8.62FGKK44 pKa = 9.42MAMSNRR50 pKa = 11.84SGGGGGLKK58 pKa = 9.62IVPVPGGFKK67 pKa = 9.58IVHH70 pKa = 6.05PNKK73 pKa = 9.96GRR75 pKa = 11.84YY76 pKa = 9.34NYY78 pKa = 10.03MSVLEE83 pKa = 4.5KK84 pKa = 10.8GRR86 pKa = 11.84SRR88 pKa = 11.84YY89 pKa = 9.89DD90 pKa = 3.08MKK92 pKa = 10.87EE93 pKa = 3.65ALLSGPRR100 pKa = 11.84ARR102 pKa = 11.84TGKK105 pKa = 9.85NGRR108 pKa = 11.84YY109 pKa = 8.09TIIPLTKK116 pKa = 10.44NSDD119 pKa = 3.29KK120 pKa = 11.54SPISPVNNDD129 pKa = 2.42INAVITKK136 pKa = 9.9VGSFKK141 pKa = 10.81DD142 pKa = 3.22INPRR146 pKa = 11.84GEE148 pKa = 3.65IVTRR152 pKa = 11.84NRR154 pKa = 11.84YY155 pKa = 8.59SYY157 pKa = 10.66RR158 pKa = 11.84IDD160 pKa = 3.34PGMTGKK166 pKa = 11.03GNVFASEE173 pKa = 3.68QVYY176 pKa = 10.89RR177 pKa = 11.84NGQVKK182 pKa = 8.16RR183 pKa = 11.84TFLKK187 pKa = 10.62FLTVSEE193 pKa = 4.35KK194 pKa = 10.7SKK196 pKa = 10.81GMFYY200 pKa = 10.59PRR202 pKa = 11.84IPAYY206 pKa = 10.61NIISGIRR213 pKa = 11.84QRR215 pKa = 11.84IEE217 pKa = 3.41RR218 pKa = 11.84KK219 pKa = 9.71LRR221 pKa = 11.84GNEE224 pKa = 3.29IRR226 pKa = 11.84SAVAMDD232 pKa = 4.05VKK234 pKa = 11.11SLTLDD239 pKa = 3.75LLKK242 pKa = 10.78KK243 pKa = 9.9QNKK246 pKa = 8.09SS247 pKa = 2.98

Molecular weight: 27.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

103

0

103

25486

39

1865

247.4

27.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.67 ± 0.356

0.628 ± 0.06

4.724 ± 0.177

7.33 ± 0.361

5.509 ± 0.256

6.137 ± 0.293

1.22 ± 0.088

7.734 ± 0.249

7.851 ± 0.335

10.292 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.499 ± 0.108

5.352 ± 0.188

4.085 ± 0.185

3.088 ± 0.337

4.846 ± 0.272

8.711 ± 0.374

5.348 ± 0.221

5.552 ± 0.208

0.942 ± 0.072

3.484 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski