Oceanicola sp. 22II-s10i

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Oceanicola; unclassified Oceanicola

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4471 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A254QXC6|A0A254QXC6_9RHOB Luciferase OS=Oceanicola sp. 22II-s10i OX=1317116 GN=ATO6_22275 PE=4 SV=1
MM1 pKa = 7.78IYY3 pKa = 10.76GEE5 pKa = 4.04QSYY8 pKa = 7.85ATSTSHH14 pKa = 7.71DD15 pKa = 3.92MIQLYY20 pKa = 10.39MNFFNMADD28 pKa = 3.28ARR30 pKa = 11.84GAKK33 pKa = 9.67AVEE36 pKa = 5.17DD37 pKa = 3.94INVMGNVLNGASVNGNALDD56 pKa = 3.57NEE58 pKa = 4.25MRR60 pKa = 11.84GNVGNDD66 pKa = 3.32TLSGGSGNDD75 pKa = 3.3EE76 pKa = 3.72IFGKK80 pKa = 10.69GGNDD84 pKa = 3.3KK85 pKa = 11.17LSGGIGNDD93 pKa = 3.63FLSGDD98 pKa = 4.02AGNDD102 pKa = 3.9FIDD105 pKa = 4.62GGNDD109 pKa = 2.71QDD111 pKa = 4.31TIYY114 pKa = 10.8GGSGNDD120 pKa = 3.85SLWGASGNDD129 pKa = 3.46VIYY132 pKa = 10.56GGSEE136 pKa = 4.01DD137 pKa = 5.31DD138 pKa = 3.76VLKK141 pKa = 11.42GEE143 pKa = 5.32DD144 pKa = 3.76GNDD147 pKa = 3.54YY148 pKa = 11.13LDD150 pKa = 4.27GGSGFDD156 pKa = 4.78RR157 pKa = 11.84MDD159 pKa = 3.54GGAGNDD165 pKa = 3.72TLKK168 pKa = 10.91GGEE171 pKa = 4.27DD172 pKa = 3.34MAGGEE177 pKa = 4.59GNDD180 pKa = 3.32TFYY183 pKa = 11.55VVDD186 pKa = 4.25DD187 pKa = 4.14AQIVTEE193 pKa = 4.63ALNEE197 pKa = 4.2GQDD200 pKa = 3.74VVRR203 pKa = 11.84TTLTEE208 pKa = 3.96YY209 pKa = 10.65TLGANLEE216 pKa = 4.15EE217 pKa = 4.53LVYY220 pKa = 10.79EE221 pKa = 4.57GDD223 pKa = 3.34VSATVSFEE231 pKa = 4.15GNASDD236 pKa = 4.67NYY238 pKa = 10.04ISARR242 pKa = 11.84NSAHH246 pKa = 6.64AILWGGDD253 pKa = 3.0GWDD256 pKa = 4.74DD257 pKa = 3.66LHH259 pKa = 7.54GSNTGYY265 pKa = 10.71DD266 pKa = 3.46QLYY269 pKa = 9.86GGSGDD274 pKa = 3.86DD275 pKa = 3.68VIYY278 pKa = 10.21GYY280 pKa = 11.23GGDD283 pKa = 4.18DD284 pKa = 4.97FINGGTGHH292 pKa = 7.72DD293 pKa = 4.02YY294 pKa = 10.73MRR296 pKa = 11.84GGTGNDD302 pKa = 3.26TYY304 pKa = 11.69VVDD307 pKa = 4.42SNLDD311 pKa = 3.79RR312 pKa = 11.84IIEE315 pKa = 4.18YY316 pKa = 10.89ANEE319 pKa = 4.7GYY321 pKa = 10.65DD322 pKa = 3.0IARR325 pKa = 11.84ITNQYY330 pKa = 10.35FKK332 pKa = 11.21LLDD335 pKa = 3.36TSMVEE340 pKa = 3.99EE341 pKa = 4.58LQATTNNGHH350 pKa = 6.5MIAGNMYY357 pKa = 9.86ANTIRR362 pKa = 11.84GLNGEE367 pKa = 4.68DD368 pKa = 3.35VLQGFDD374 pKa = 3.54GNDD377 pKa = 3.25YY378 pKa = 11.25LVGNGGNDD386 pKa = 3.46SLDD389 pKa = 3.76GGSGNDD395 pKa = 3.58TMLGGYY401 pKa = 10.56GNDD404 pKa = 3.86TYY406 pKa = 10.71WIDD409 pKa = 3.44SQYY412 pKa = 11.76DD413 pKa = 3.52KK414 pKa = 10.89IVEE417 pKa = 4.08YY418 pKa = 10.97SSGGFDD424 pKa = 2.85TAYY427 pKa = 9.81IKK429 pKa = 10.77NSYY432 pKa = 10.21YY433 pKa = 11.19SFIGSEE439 pKa = 3.89NVEE442 pKa = 3.95RR443 pKa = 11.84MIASSNWNTTIHH455 pKa = 5.88GTNGHH460 pKa = 5.26NRR462 pKa = 11.84ILGGAKK468 pKa = 9.71NDD470 pKa = 3.9LLSGRR475 pKa = 11.84GGNDD479 pKa = 3.26NIHH482 pKa = 6.62GGGGADD488 pKa = 2.71RR489 pKa = 11.84VYY491 pKa = 11.13GGTGNDD497 pKa = 3.15QFIFQNNRR505 pKa = 11.84SNTGSKK511 pKa = 10.88VMDD514 pKa = 4.35FVHH517 pKa = 7.19GEE519 pKa = 4.04DD520 pKa = 5.34KK521 pKa = 10.81IVLDD525 pKa = 6.01SDD527 pKa = 4.76GQWGWAAMGNGTVVIQSGNGGGSITVYY554 pKa = 11.14ADD556 pKa = 3.0NLYY559 pKa = 10.53YY560 pKa = 10.87GSDD563 pKa = 3.35IQLMSDD569 pKa = 3.27STIANLII576 pKa = 3.93

Molecular weight: 60.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A254R370|A0A254R370_9RHOB Uncharacterized protein OS=Oceanicola sp. 22II-s10i OX=1317116 GN=ATO6_17525 PE=4 SV=1
MM1 pKa = 7.48PAAHH5 pKa = 6.95SFRR8 pKa = 11.84TRR10 pKa = 11.84LRR12 pKa = 11.84RR13 pKa = 11.84FQVQARR19 pKa = 11.84RR20 pKa = 11.84SIPPGLRR27 pKa = 11.84LVLGILLIIGGFLGFLPILGFWMLPLGIAIAALDD61 pKa = 3.96VVPVWRR67 pKa = 11.84RR68 pKa = 11.84MVSLRR73 pKa = 11.84RR74 pKa = 11.84PRR76 pKa = 11.84RR77 pKa = 11.84PRR79 pKa = 3.53

Molecular weight: 9.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4471

0

4471

1400842

32

10931

313.3

33.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.544 ± 0.046

0.865 ± 0.013

6.134 ± 0.03

5.964 ± 0.03

3.639 ± 0.023

9.079 ± 0.036

1.994 ± 0.018

5.112 ± 0.028

2.961 ± 0.036

9.814 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.895 ± 0.026

2.426 ± 0.022

5.199 ± 0.024

2.885 ± 0.017

7.025 ± 0.038

4.914 ± 0.024

5.535 ± 0.039

7.412 ± 0.029

1.388 ± 0.015

2.216 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski