Enterococcus florum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3833 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P5P9L3|A0A4P5P9L3_9ENTE Proline reductase cluster protein PrdD OS=Enterococcus florum OX=2480627 GN=prdD PE=4 SV=1
MM1 pKa = 7.29KK2 pKa = 9.77WVILILFLVALGLAAVIFLYY22 pKa = 10.44INRR25 pKa = 11.84SDD27 pKa = 4.2NNDD30 pKa = 3.51TASSEE35 pKa = 4.29STSQVTGSTQASSSEE50 pKa = 4.01EE51 pKa = 3.89GAEE54 pKa = 4.0TGEE57 pKa = 4.12PSSVTFEE64 pKa = 3.84KK65 pKa = 10.9GKK67 pKa = 10.94VYY69 pKa = 10.23TGSDD73 pKa = 3.32DD74 pKa = 3.48QYY76 pKa = 10.57GYY78 pKa = 11.21SITTYY83 pKa = 10.16QDD85 pKa = 2.9SEE87 pKa = 4.91NIVVSHH93 pKa = 6.77GPLGQDD99 pKa = 3.39GSAAPMSDD107 pKa = 2.95AASLVPQTVDD117 pKa = 3.2TQPIQINEE125 pKa = 3.84NGEE128 pKa = 3.97IKK130 pKa = 10.17EE131 pKa = 4.19VQVNTEE137 pKa = 4.26LVLSNTPNAASLGFFVPFNSSNTKK161 pKa = 9.37IFAYY165 pKa = 7.56NTPSGNIALAFTSQGTIDD183 pKa = 3.48QYY185 pKa = 11.32QVVEE189 pKa = 4.7FTLSHH194 pKa = 7.04DD195 pKa = 4.03SEE197 pKa = 4.76

Molecular weight: 21.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P5PA76|A0A4P5PA76_9ENTE Uncharacterized protein OS=Enterococcus florum OX=2480627 GN=NRIC_27410 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 9.16RR10 pKa = 11.84KK11 pKa = 9.12RR12 pKa = 11.84QKK14 pKa = 9.38VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.73GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3833

0

3833

1160261

39

2921

302.7

33.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.409 ± 0.041

0.786 ± 0.014

5.228 ± 0.038

7.478 ± 0.047

4.565 ± 0.034

6.739 ± 0.036

1.879 ± 0.017

7.413 ± 0.038

6.504 ± 0.035

9.99 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.77 ± 0.022

4.255 ± 0.027

3.573 ± 0.02

4.352 ± 0.036

4.048 ± 0.029

5.876 ± 0.028

5.694 ± 0.033

6.92 ± 0.033

0.908 ± 0.014

3.613 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski