Verrucomicrobiaceae bacterium

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Verrucomicrobiae; Verrucomicrobiales; Verrucomicrobiaceae; unclassified Verrucomicrobiaceae

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5527 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q2XX62|A0A4Q2XX62_9BACT D-arabino 3-hexulose 6-phosphate aldehyde lyase OS=Verrucomicrobiaceae bacterium OX=2026800 GN=EOP86_22540 PE=4 SV=1
AA1 pKa = 7.4TGVNGDD7 pKa = 3.43QADD10 pKa = 3.95NSAHH14 pKa = 5.7QSGAAYY20 pKa = 9.57IFTVADD26 pKa = 3.56YY27 pKa = 9.96EE28 pKa = 4.43YY29 pKa = 11.25SLLTTAVNGTVTGAGEE45 pKa = 4.17YY46 pKa = 10.94AFGTTATLTATPDD59 pKa = 2.95AGYY62 pKa = 11.12VFTGWTGDD70 pKa = 3.73AGGTDD75 pKa = 3.38NPLPVLMDD83 pKa = 3.67SDD85 pKa = 3.87RR86 pKa = 11.84TIGAVFGPDD95 pKa = 3.3SSDD98 pKa = 3.38ADD100 pKa = 3.82GDD102 pKa = 4.03GLSAYY107 pKa = 9.89LEE109 pKa = 3.89RR110 pKa = 11.84VVYY113 pKa = 8.04GTNPLLADD121 pKa = 3.57TDD123 pKa = 4.25GDD125 pKa = 4.13SLSDD129 pKa = 3.3KK130 pKa = 11.31GEE132 pKa = 4.07VEE134 pKa = 3.85ITLTNPLVNDD144 pKa = 3.78SDD146 pKa = 4.54GDD148 pKa = 3.85WVLDD152 pKa = 3.94ADD154 pKa = 4.84EE155 pKa = 6.14DD156 pKa = 4.49PDD158 pKa = 5.55GDD160 pKa = 3.98GFTNGQEE167 pKa = 3.96LTLFLTDD174 pKa = 4.31PNSGADD180 pKa = 3.6CFTTALEE187 pKa = 4.41YY188 pKa = 9.62TASAHH193 pKa = 6.14SLTFATVAGRR203 pKa = 11.84TYY205 pKa = 10.28QVEE208 pKa = 4.25RR209 pKa = 11.84ALKK212 pKa = 10.2PGDD215 pKa = 3.3PAAWSEE221 pKa = 4.13RR222 pKa = 11.84FSFIASGGAVTVPLGPPSSTRR243 pKa = 11.84WFYY246 pKa = 10.99RR247 pKa = 11.84VRR249 pKa = 11.84VSLNN253 pKa = 2.94

Molecular weight: 26.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q2XNC4|A0A4Q2XNC4_9BACT DUF1778 domain-containing protein OS=Verrucomicrobiaceae bacterium OX=2026800 GN=EOP86_26125 PE=4 SV=1
MM1 pKa = 7.94PSRR4 pKa = 11.84AAARR8 pKa = 11.84RR9 pKa = 11.84VVVRR13 pKa = 11.84VPVKK17 pKa = 10.0AVRR20 pKa = 11.84NVRR23 pKa = 11.84RR24 pKa = 11.84APKK27 pKa = 8.34VAHH30 pKa = 4.97VAGRR34 pKa = 11.84VVNAAAPQPRR44 pKa = 11.84VSRR47 pKa = 11.84VRR49 pKa = 11.84LRR51 pKa = 11.84RR52 pKa = 11.84LLVPPLLLPNRR63 pKa = 11.84LRR65 pKa = 11.84RR66 pKa = 11.84VQPLRR71 pKa = 11.84QRR73 pKa = 11.84LPPRR77 pKa = 11.84VNPLHH82 pKa = 7.18RR83 pKa = 11.84LRR85 pKa = 11.84HH86 pKa = 5.47LPRR89 pKa = 11.84ALPRR93 pKa = 11.84HH94 pKa = 5.81RR95 pKa = 11.84RR96 pKa = 11.84PLLLRR101 pKa = 11.84RR102 pKa = 11.84LRR104 pKa = 11.84LPP106 pKa = 3.56

Molecular weight: 12.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5527

0

5527

1744395

21

2852

315.6

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.309 ± 0.046

0.96 ± 0.013

5.294 ± 0.027

5.789 ± 0.032

3.757 ± 0.018

8.812 ± 0.038

2.137 ± 0.018

4.275 ± 0.028

3.57 ± 0.032

10.28 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.188 ± 0.016

2.911 ± 0.025

6.169 ± 0.031

3.24 ± 0.017

6.825 ± 0.035

6.098 ± 0.028

5.811 ± 0.033

6.712 ± 0.029

1.7 ± 0.017

2.165 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski