Serratia symbiotica str. Tucson

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Yersiniaceae; Serratia; Serratia symbiotica

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2157 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E9CJX1|E9CJX1_9GAMM Putative respiratory NADH dehydrogenase 2/cupric reductase OS=Serratia symbiotica str. Tucson OX=914128 GN=ndh PE=4 SV=1
MM1 pKa = 6.91NTQNVNVNTAAQEE14 pKa = 3.77RR15 pKa = 11.84SEE17 pKa = 4.83RR18 pKa = 11.84YY19 pKa = 9.16GKK21 pKa = 10.55KK22 pKa = 9.91SALKK26 pKa = 10.62NSGLSDD32 pKa = 3.61DD33 pKa = 3.6QVYY36 pKa = 11.3DD37 pKa = 3.99LFCHH41 pKa = 5.9VCLACGGRR49 pKa = 11.84AIGTMEE55 pKa = 4.53SPALPADD62 pKa = 3.65AEE64 pKa = 4.34YY65 pKa = 11.25LSIQLGDD72 pKa = 3.83FDD74 pKa = 5.87SLAVWLVDD82 pKa = 3.37GWPVAASDD90 pKa = 3.53EE91 pKa = 4.4AVWIDD96 pKa = 2.94WCIAEE101 pKa = 4.15WW102 pKa = 3.51

Molecular weight: 11.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E9CN22|E9CN22_9GAMM Aspartate--tRNA ligase OS=Serratia symbiotica str. Tucson OX=914128 GN=aspS PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.06GFRR19 pKa = 11.84ARR21 pKa = 11.84MTNKK25 pKa = 9.98NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.36GRR39 pKa = 11.84TRR41 pKa = 11.84LTVSKK46 pKa = 10.95

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2098

59

2157

521053

9

1486

241.6

26.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.418 ± 0.052

1.186 ± 0.022

5.201 ± 0.047

5.631 ± 0.057

3.7 ± 0.034

7.224 ± 0.05

2.426 ± 0.026

5.882 ± 0.041

4.527 ± 0.052

11.004 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.788 ± 0.03

3.762 ± 0.034

4.326 ± 0.036

4.787 ± 0.049

5.978 ± 0.049

5.835 ± 0.045

5.109 ± 0.038

7.082 ± 0.055

1.284 ± 0.027

2.849 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski