Leptospira phage vb_LkmZ_Bejolso9-LE1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified dsDNA phages

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5VY87|S5VY87_9VIRU Uncharacterized protein OS=Leptospira phage vb_LkmZ_Bejolso9-LE1 OX=1334244 GN=LEP1GSC052_0086 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.57AKK4 pKa = 10.51DD5 pKa = 3.89LFFKK9 pKa = 10.52RR10 pKa = 11.84HH11 pKa = 5.63PSALKK16 pKa = 10.15NFVYY20 pKa = 9.07DD21 pKa = 3.6TLEE24 pKa = 4.1KK25 pKa = 10.75LFQNDD30 pKa = 2.4SDD32 pKa = 5.53LEE34 pKa = 4.37VEE36 pKa = 4.34TLNSIVRR43 pKa = 11.84IQKK46 pKa = 10.17EE47 pKa = 4.41SVKK50 pKa = 10.88NDD52 pKa = 4.48LIYY55 pKa = 10.98SNEE58 pKa = 3.67YY59 pKa = 10.38RR60 pKa = 11.84INQLMTSLIEE70 pKa = 3.84HH71 pKa = 6.33SKK73 pKa = 10.98DD74 pKa = 3.56FEE76 pKa = 4.37IKK78 pKa = 10.22SLSKK82 pKa = 10.4KK83 pKa = 10.06IYY85 pKa = 9.47IGVTNTYY92 pKa = 8.16TFEE95 pKa = 4.26AKK97 pKa = 10.24AVFPEE102 pKa = 4.47DD103 pKa = 3.94DD104 pKa = 3.67EE105 pKa = 4.94SLIEE109 pKa = 4.11INFGFYY115 pKa = 10.95YY116 pKa = 10.76LINLYY121 pKa = 10.78GDD123 pKa = 3.85VITRR127 pKa = 11.84LGLTIRR133 pKa = 11.84ADD135 pKa = 4.06FNEE138 pKa = 4.23SSQVLDD144 pKa = 4.85VIDD147 pKa = 4.71SDD149 pKa = 3.64IQKK152 pKa = 10.37GINAIISNFNYY163 pKa = 9.13FQKK166 pKa = 10.71KK167 pKa = 8.79IVSLEE172 pKa = 3.84KK173 pKa = 11.07GPDD176 pKa = 3.24FEE178 pKa = 5.6YY179 pKa = 10.16WGNDD183 pKa = 2.63NWNEE187 pKa = 3.81RR188 pKa = 11.84MEE190 pKa = 4.43NMAFRR195 pKa = 11.84IKK197 pKa = 10.5SIMYY201 pKa = 9.33LFVLLHH207 pKa = 5.9EE208 pKa = 5.06VGHH211 pKa = 6.86HH212 pKa = 6.65IYY214 pKa = 10.98DD215 pKa = 3.65HH216 pKa = 6.76LKK218 pKa = 10.15GFQSYY223 pKa = 10.67SLPDD227 pKa = 3.47IPNQILASIDD237 pKa = 3.45QNKK240 pKa = 9.78YY241 pKa = 10.95NIDD244 pKa = 3.55QIQEE248 pKa = 3.92FEE250 pKa = 4.41ADD252 pKa = 4.04LFAANYY258 pKa = 9.36LLNIRR263 pKa = 11.84DD264 pKa = 3.85YY265 pKa = 11.3TDD267 pKa = 4.37DD268 pKa = 4.97DD269 pKa = 5.23SILKK273 pKa = 10.53LLDD276 pKa = 3.33NTTCSLGILITLHH289 pKa = 5.69LTAGVLGDD297 pKa = 3.71IEE299 pKa = 4.66IGNEE303 pKa = 3.75NHH305 pKa = 7.0PPLSDD310 pKa = 4.49RR311 pKa = 11.84IQIIILFIQQKK322 pKa = 9.65VGQSEE327 pKa = 4.18FNEE330 pKa = 4.8IIDD333 pKa = 3.76TFQNLVYY340 pKa = 10.55RR341 pKa = 11.84IVNFSS346 pKa = 3.0

Molecular weight: 40.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5WIY1|S5WIY1_9VIRU Uncharacterized protein OS=Leptospira phage vb_LkmZ_Bejolso9-LE1 OX=1334244 GN=LEP1GSC052_0081 PE=4 SV=1
MM1 pKa = 7.56EE2 pKa = 5.27NYY4 pKa = 9.94EE5 pKa = 4.32KK6 pKa = 10.26IKK8 pKa = 10.51RR9 pKa = 11.84LYY11 pKa = 8.44PPYY14 pKa = 9.67WVSDD18 pKa = 3.47VCKK21 pKa = 10.29SLRR24 pKa = 11.84GRR26 pKa = 11.84HH27 pKa = 5.29KK28 pKa = 11.23NIDD31 pKa = 3.22KK32 pKa = 10.7KK33 pKa = 11.1LNSKK37 pKa = 8.23RR38 pKa = 11.84TPKK41 pKa = 9.92PRR43 pKa = 11.84TWKK46 pKa = 10.71LCIEE50 pKa = 4.31YY51 pKa = 10.11NAQKK55 pKa = 10.44LRR57 pKa = 11.84RR58 pKa = 11.84LHH60 pKa = 6.84AKK62 pKa = 10.1DD63 pKa = 3.01GRR65 pKa = 11.84KK66 pKa = 9.56RR67 pKa = 11.84KK68 pKa = 9.57EE69 pKa = 4.01NNQPWNAFVTYY80 pKa = 10.15QKK82 pKa = 10.84EE83 pKa = 3.99KK84 pKa = 10.62LSRR87 pKa = 11.84KK88 pKa = 9.14FDD90 pKa = 3.88EE91 pKa = 4.36NSLWKK96 pKa = 10.36HH97 pKa = 5.47LEE99 pKa = 3.46NRR101 pKa = 11.84ILKK104 pKa = 9.81KK105 pKa = 10.41INAHH109 pKa = 5.58NFPKK113 pKa = 10.41EE114 pKa = 4.03RR115 pKa = 11.84GALWDD120 pKa = 3.57YY121 pKa = 10.74CYY123 pKa = 10.6HH124 pKa = 5.88RR125 pKa = 11.84QIRR128 pKa = 11.84KK129 pKa = 9.15LKK131 pKa = 10.27NIHH134 pKa = 6.57PDD136 pKa = 3.14LRR138 pKa = 11.84EE139 pKa = 3.8RR140 pKa = 11.84PADD143 pKa = 3.59YY144 pKa = 10.53EE145 pKa = 4.0KK146 pKa = 10.99RR147 pKa = 11.84FANRR151 pKa = 11.84QMEE154 pKa = 4.39KK155 pKa = 10.93LKK157 pKa = 10.75FLYY160 pKa = 10.14NKK162 pKa = 9.84KK163 pKa = 10.31KK164 pKa = 10.38PEE166 pKa = 3.83NEE168 pKa = 3.46EE169 pKa = 3.84WKK171 pKa = 10.37FWWLAQLRR179 pKa = 11.84KK180 pKa = 9.8LHH182 pKa = 6.09ARR184 pKa = 11.84YY185 pKa = 9.87KK186 pKa = 10.86GVLGVNNKK194 pKa = 8.64NRR196 pKa = 11.84FKK198 pKa = 11.22DD199 pKa = 3.56NPKK202 pKa = 9.66WYY204 pKa = 10.45

Molecular weight: 25.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34

0

34

13158

42

2260

387.0

44.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.264 ± 0.225

0.973 ± 0.12

5.259 ± 0.292

6.407 ± 0.624

5.578 ± 0.234

5.495 ± 0.672

1.695 ± 0.203

8.147 ± 0.425

8.094 ± 0.563

9.941 ± 0.534

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.497 ± 0.08

6.954 ± 0.372

3.481 ± 0.261

3.595 ± 0.162

4.309 ± 0.281

8.649 ± 0.451

5.525 ± 0.598

4.651 ± 0.254

1.094 ± 0.159

4.393 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski